The basic study of protective effects by carnosic acid against anthrocyclin-induced cardiotoxicity

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Iwate Medical University
• Principal Investigator: ISHIDA Yoji Iwate Medical University, 医学部, 教授 (70151389)
• Project Period (FY): 2008 – 2010
• Keywords: アントラサイクリン / 心筋障害 / カルノシン酸 / 抗酸化作用

Research Abstract

We evaluated the oxidant effects of doxorubicine (DOX) on murine cardio-myocytic cell line. DOX did not have cytotoxic effects on HL-1 cells (～100 ng/ml). Therefore, we have evaluated the cytotoxic effect of acetaminophen (AA), which has much more oxidant effects, instead of DXR. The cytotoxic effect of AA was reduced by the addition of carnosic acid (CA). The RNA was extracted from HL-1 cells, cultured with CA, which concentration showed the maximum anti-oxidant effect. The quantitative RT-PCR was performed, resulting in that phase II enzymes were not highly expressed significantly. These results suggest that anti-oxidant effect of CA might be independent on the expression of Phase II enzymes.

Research Products

• [Journal Article] Deguelin suppresses cell proliferation via the inhibition of surviving expression and STAT3 phosphorylation in HTLV-1- transformed T cells. (2010)
  • Author(s): Ito S, Oyake T, Murai K, Ishida Y
  • Journal Title: Leuk Res. 34(3) Pages: 352-357
• [Journal Article] Factor XII Ofunato : Lys346Asn mutation associated with blood coagulation factor XII deficiency causes impaired secretion through aproteasome- mediated degradation. (2010)
  • Author(s): Suzuki K, Murai K, Suwabe A, Ishida Y
  • Journal Title: Thromb Res 125(5) Pages: 438-443
• [Journal Article] Clinical efficacy and safety of biapenem for febrile neutropenia in patients with underlying hematopoietic diseases : a multi-institutional study (2010)
  • Author(s): Nakagawa Y, Suzuki K, Ishida Y, Urabe A, et al.
  • Journal Title: J Infect Chemother in press
• [Journal Article] A palmitoyl conjugate of insect pentapeptide Yamamarin arrests cell proliferation and respiration (2010)
  • Author(s): Sato Y, Yang P, An Y, Matsukawa K, Ito K, Imanishi S, Matsuda H, Uchiyama Y, Imai K, Ito S, Ishida Y, Suzuki K
  • Journal Title: Peptides 31 Pages: 827-833
• [Journal Article] Drug susceptibility and clonality of methicillin-resistant Staphylococcus epidermidis in hospitalized patients with hematological malignancies (2010)
  • Author(s): Nomura K. Mizumachi E. Yamashita M. Ohshiro M. Komori T. Sugai M. Taniwaki M. Ishida Y.
  • Journal Title: Ir J Med Sci 179(3) Pages: 351-356
• [Journal Article] Japan Haematology/Oncology Study (J-HOST) Group. Risk factors for infection in haematology patients treated with rituximab. (2009)
  • Author(s): Kanbayashi Y, Nomura K, Fujimoto Y, Yamashita M, Ohshiro M, Okamoto K, Matsumoto Y, Horiike S, Takagi T, Ishida Y, Taniwaki M
  • Journal Title: Eur J Haematol 82(1) Pages: 26-30
• [Journal Article] Japan Hematology/Oncology Study (J-HOST) Group Kyoto. Absence of pseudomembranes in Clostridium difficile-associated diarrhea in patients using immunosuppression agents. (2009)
  • Author(s): Nomura K, Fujimoto Y, Yamashita M, Morimoto Y, Ohshiro M, Sato K, Oyake T, Kowata S, Konishi H, Yoshikawa T, Ishida Y, Taniwaki M
  • Journal Title: Scand J Gastroenterol 44(1) Pages: 74-78
• [Presentation] Bortezomib induced thrombocytopenia might be due to the inhibition of proplatelet formation of megakaryocyte. (2010)
  • Author(s): Murai K, Kowata S, Abo A, Oyake T, Ishida Y, et al.
  • Organizer: 52^<nd> American Society of Hematology Annual Meeting and Exposition December 6^<th>
  • Place of Presentation: Orland, USA
  • Year and Date: 20100000
• [Presentation] Micafungin versus voriconazole for empirical anti- fungal therapy in febrile neutron- penic patients with acute myeloid leukemia : A Randomized, controlled study. (2010)
  • Author(s): Oyake T, Kowata S, Murai K, Ishida Y, et al.
  • Organizer: 52^<nd> American Society of Hematology Annual Meeting and Exposition December 4^<th>
  • Place of Presentation: Orland, USA
  • Year and Date: 20100000
• [Presentation] Resveratrol Induces cell growth arrest and cell death of adult T- cell leukemia cells via deacetylation of STAT3 bySIRT1. (2010)
  • Author(s): Ito S, Oyake T, Murai K, Ishida Y.
  • Organizer: 52^<nd> American Society of Hematology Annual Meeting and Exposition December 5^<th>
  • Place of Presentation: Orland, USA
  • Year and Date: 20100000
• [Presentation] The role of actin nucleating factors, Arp3 and mDia1, during proplatelet formation of megakaryocytes. (2009)
  • Author(s): Kowata S, Oyake T, Murai K, Ishida Y, et al.
  • Organizer: 51^<st> American Society of Hematology Annual Meeting and Exposition December 6^<th>
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20090000
• [Presentation] Deguelin induced cell growth arrest and cell death via the suppression of BCR-ABL and Survivin expression in BCR-ABL-T315 mutant cells. (2009)
  • Author(s): Oyake T, Ito S, Kowata S, Ishida Y, et al.
  • Organizer: 51^<st> American Societyof Hematology Annual Meeting and Exposition December 7^<th>
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20090000
• [Presentation] Deguelin inducs cell growth arrestand cell death by destabilizing phosphorylated STAT 3 and survivinin adult T-cell leukemia cells. (2009)
  • Author(s): Ito S, Oyake T, Murai K, Ishida Y.
  • Organizer: 51^<st> American Society of HematologyAnnual Meeting and Exposition December 7^<th>
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20090000
• [Book] 臨床検査ガイド,東京他 14件 (2010)
  • Author(s): 石田陽治
  • Publisher: 文光堂