2010 Fiscal Year Final Research Report
Clarification of the mechanism of differentiation from hematopoietic stem cell-derived cells to hepatic stellate cells
Project/Area Number |
20591150
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Mie University |
Principal Investigator |
MASUYA Masahiro Mie University, 大学院・医学系研究科, 准教授 (30281083)
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Project Period (FY) |
2008 – 2010
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Keywords | 単球 / 分化 / 肝星細胞 / MCP-1 / CCR2 |
Research Abstract |
We previously clarified that hematopoietic stem cell-derived cells were abele to differentiate into hepatic stellate cells (HpSCs) during carbon tetrachloride (CCl_4)-induced iver injury. Which lineage of cells are true precursors of HpSCs remains unknown. We idoptively transferred neutrophils, monocytes, eosinophils, B cells, or T cells, which were isolated rom enhanced green fluorescent protein (EGFP)-transgenic mice, into CCl_4-injured non-transgenic nice. In the livers of mice given monocytes, EGFP^+ HpSCs were detected. This suggest that a proportion of monocytes are precursors of HpSCs. Monocyte chemoattractant protein-1 (MCP-1) is bought to be associated with the invasion of monocytes into injured liver. CCR2, a receptor for vICP-1, is expressed on monocytes. We examined the effect of CCR2 antagonist against the lifferentiation of monocytes into HpSCs and found that the antagonist suppressed the number of EGFP^+ HpSCs and the area of fibrosis in injured liver.
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[Journal Article] Thrombocytopenia in mice lacking the carboxy-termainal regulatory domain of the Ets transcription factor Flil2010
Author(s)
Moussa O, LaRue AC, Abangan RS Jr, Williams CR, Zhang XK, Masuya M, Gong YZ, Spyropoulos DD, Ogawa M, Gilkeson G, Watson DK
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Journal Title
Mol Cell Biol Vol.30, No.21
Pages: 5194-5206
Peer Reviewed
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[Journal Article] Diagnostic value of PCR analysis of bacteria and fungi from blood in empiric-therapy-resistant febrile neutropenia2010
Author(s)
Nakamura A, Sugimoto Y, Ohishi K, Sugawara Y, Fujieda A, Monma F, Suzuki K, Masuya M, Nakase K, Matsushima Y, Wada H, Katayama N, Nobori T
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Journal Title
J Clin Microbiol Vol.48, No.6
Pages: 2030-2036
Peer Reviewed
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[Journal Article] A potential activity of valproic acid in the stimulation of interleukin-3-mediated megakaryopoiesis and erythropoiesis2010
Author(s)
Liu B, Ohishi K, Yamamura K, Suzuki K, Monma F, Ino K, Nishii K, Masuya M, Sekine T, Heike Y, Takaue Y, Katayama N
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Journal Title
Exp Hematol Vol.38, No.8
Pages: 685-695
Peer Reviewed
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[Journal Article] The effectiveness of measuring for fragmented red cells using an automated hematology analyzer in patients with thrombotic microangiopathy2009
Author(s)
Abe Y, Wada H, Yamada E, Noda M, Ikejiri M, NIshioka J, Kobayashi T, Matsumoto T, Masuya M, Isaji S, Usui M, Uemoto S, Katayama N, Nobori T
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Journal Title
Clin Appl Thromb Hemost Vol.15, No.2
Pages: 257-262
Peer Reviewed
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[Journal Article] Dual topology of functional Toll-like receptor 3 expression in human hepatocellular carcinoma2008
Author(s)
Yoneda K, Sugimoto K, Shiraki K, Tanaka J, Beppu T, Fuke H, Yamamoto N, Masuya M, Horie R, Uchida K, Takei Y
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Journal Title
differential signaling mechanisms of TLR3-induced NF-kappaB activation and apoptosis Vol.33, No.5
Pages: 929-936
Peer Reviewed
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[Journal Article] Deletion of chromosome arm 15q in a case of minimally differentiated hypoplastic AML-M02008
Author(s)
Fujieda A, Masuya M, Kitano S, Miyazaki K, Yazaki A, Sugimoto Y, Usui E, Miyata E, Shibasaki T, Yamamura K, Ohishi K, Nishii K, Nakase K, Takeuchi Tm, Katayama N
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Journal Title
Cancer Genet Cytogenet Vol.184, No.1
Pages: 57-61
Peer Reviewed
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[Journal Article] ADAMTSI3 related markers and von Willebrand factor in plasma from patients with thrombotic microangiopathy (TMA)2008
Author(s)
Kobayashi T, Wada H, Nishioka J, Yamamoto M, Matsumoto T, Tamara T, Nomura S, Masuya M, Mori Y, Nakatani K, Nishikawa M, Katayama N, Nobori T
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Journal Title
Thromb Res Vol.121, No.6
Pages: 849-854
Peer Reviewed
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[Presentation] Transfusion of platelet concentrates washed twice with M-sol provided adequatepost-transfusion platelet count increments in an anhaptoglobinemic patient with anti-haptoglobin antibody2009
Author(s)
Tanaka Y, Kassai C, Abe K, Nishio M, Maruyama M, Kamada N, Matsumoto T, Masuya M, Nishioka Y, Tatara Y, Ohishi K
Organizer
The XXth Regional Congress of the International Society Blood Transfusion (ISBT) Asia
Place of Presentation
Nagoya, Japan (Poster)
Year and Date
20091114-20091118
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