2010 Fiscal Year Final Research Report
Function and intracellular signaling of human umbilical cord blood-derived cultured basophils
| Project/Area Number |
20591183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Gunma University |
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Principal Investigator |
ISHIZUKA Tamotsu Gunma University, 大学院・医学系研究科, 講師 (50302477)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ヒト好塩基球 / IgE / 臍帯血 / CD34 / IL-4 / IL-8 / ロイコトリエン |
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| Research Abstract |
Human umbilical cord blood-derived mononuclear cells were cultured in the presence of 10 ng/ml IL-3 for 2 weeks. Alcian blue stained cells were isolated after the culture. These premature basophils were passively sensitized with human IgE and stimulated by anti-human IgE polyclonal antibody (1 μg/ml). Substantial amount of IL-8 was detected in cell supernatants at 3h after the stimulation. Although the expression of IgE Fc receptors in these cells was analyzed by flowcytometry, the expression was a little. To increase the expression of IgE Fc receptors, CD34+ cells isolated from human cord blood were cultured in the presence of 500 ng/ml IgE as well as 10 ng/ml IL-3 for 2 weeks. Substantial expression of IgE Fc receprors was observed on cell surface of CD34+ cell-derived premature basophils cultured in this condition. IL-4, IL-8, and leukotriene C4 were detected in the supernatants of CD34+ cell-derived premature basophils following the stimulation of anti-IgE.
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Research Products
(19 results)
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[Journal Article] Lysophosphatidic acid inhibits CC chemokine ligand 5/RANTES production by blocking IRF-1-mediated gene transcription in human bronchial epithelial cells.2010
Author(s)
Matsuzaki S, Ishizuka T,, Hisada T, Aoki H, Komachi M, Ichimonji I, Utsugi M, Ono A, Koga Y, Dobashi K, Kurose H, Tomura H, Mori M, Okajima F
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Journal Title
J Immunol 185
Pages: 4863-4872
Peer Reviewed
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[Journal Article] Protective effect of resolvin E1 on the development of asthmatic airway inflammation.2010
Author(s)
Aoki H, Hisada T, Ishizuka T, Utsugi M, Ono A, Koga Y, Sunaga N, Nakakura T, Okajima F, Dobashi K, Mori M
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Journal Title
Biochem Biophys Res Commun 400
Pages: 128-133
Peer Reviewed
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[Journal Article] PI3K p110beta positively regulates lipopolysaccharide-induced IL-12 production in human macrophages and dendritic cells and JNK1 plays a novel role.2009
Author(s)
Utsugi M, Dobashi K, Ono A, Ishizuka T, Matsuzaki S, Hisada T, Shimizu Y, Kawata T, Aoki H, Kamide Y, Mori M
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Journal Title
J Immunol 182
Pages: 5225-5231
Peer Reviewed
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[Journal Article] Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflammatory cytokine production in peritoneal macrophages.2009
Author(s)
Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, Okajima F
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Journal Title
J Immunol 182
Pages: 3243-3251
Peer Reviewed
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[Journal Article] The relationship between oxidative stress and acid stress in adult patients with mild asthma.2008
Author(s)
Zhao JJ, Shimizu Y, Dobashi K, Kawata T, Ono A, Yanagitani N, Kaira K, Utsugi M, Hisada T, Ishizuka T, Mori M
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Journal Title
J Investig Allergol Clin Immunol 18
Pages: 41-45
Peer Reviewed
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