• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2010 Fiscal Year Final Research Report

Molecular biological study on macrophage antimicrobial mechanism based on phospholipase A_2

Research Project

  • PDF
Project/Area Number 20591202
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Infectious disease medicine
Research InstitutionShimane University

Principal Investigator

TOMIOKA Haruaki  Shimane University, 医学部, 教授 (40034045)

Co-Investigator(Kenkyū-buntansha) SANO Chiaki  島根大学, 医学部, 准教授 (70325059)
TATANO Yutaka  島根大学, 医学部, 助教 (70432614)
SHIMIZU Toshiaki  安田女子大学, 家政学部, 准教授 (60284030)
YASUMOTO Ko  北里大学, 海洋生命科学部, 講師 (00448200)
Project Period (FY) 2008 – 2010
Keywordsマクロファージ / 抗酸菌 / 殺菌メカニズム / phospholipase A_2
Research Abstract

We investigated macrophage (MΦ) antimicrobial activity against mycobacteria based on phospholipase A_2 (PLA_2) using molecular biological techniques. (1) Among PLA_2 isotype inhibitors, cytosolic PLA_2 (cPLA_2) inhibitor mildly reduced MΦ anti-mycobacteria activity. (2) MΦ expression of cPLA_2 and secretoryPLA_2-V mRNAs were up-regulated after infection with mycobacteria. (3) cPLA_2 potentiated M Φ anti-mycobacteria activity coupled with apoptosis in the early stage. (4) GFP-tagged cPLA_2 translocated to the membrane of MΦ or CHO cells. Overall, it can be concluded that the cPLA_2-mediated antimicrobial mechanism contributes to MΦ's bactericidal and bacteriostatic function against mycobacterial pathogens but such cPLA_2-dependent mechanism does not play a central role in the MΦ antimycobacterial function.

  • Research Products

    (14 results)

All 2011 2010 2009 2008

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (1 results) Book (3 results)

  • [Journal Article] Comparative in vitro and in vivo antimicrobial activities of sitafloxacin, gatifloxacin and moxifloxacin against Mycobacterium avium.2011

    • Author(s)
      Sano C, Tatano Y, Shimizu T, Tomioka H, et al.
    • Journal Title

      Int J Antimicrob Agents. 37

      Pages: 296-301

    • Peer Reviewed
  • [Journal Article] Development of new antituberculous drugs based on bacterial virulence factors interfering with host cytokine networks.2011

    • Author(s)
      Tomioka H, Tatano Y, Sano C, Shimizu T
    • Journal Title

      J Infect Chemother (in press)

    • Peer Reviewed
  • [Journal Article] Comparative study for the virulence of Mycobacterium avium isolates from patients with nodular-bronchiectasis- and cavitary-type diseases.2010

    • Author(s)
      Tatano Y, Yasumoto K, Shimizu T, Sano C, Tomioka H, et al.
    • Journal Title

      Eur J Clin Indect Dis 29

      Pages: 801-808

    • Peer Reviewed
  • [Journal Article] Properties of immunosuppressive macrophages generated by Mycobacterium intracellulare infection in M. intracellulare-susceptible and -resistant mice.2010

    • Author(s)
      Tatano Y, Shimizu T, Tomioka H
    • Journal Title

      New Microbiologica 33

      Pages: 87-91

    • Peer Reviewed
  • [Journal Article] In vitro drug susceptibility of Mycobacterium bovis BCG Connaught and Tokyo strains.2010

    • Author(s)
      Shimizu T, Yasumoto K, Tatano Y, Tomioka H, Sano C, et al.
    • Journal Title

      J Infect 60

      Pages: 248-255

    • Peer Reviewed
  • [Journal Article] Roles of cytoplasmic phospholipase in expression of the antimicrobial activity of host macrophaes against Mycobacterium tuberculosis infection.2010

    • Author(s)
      Sano C, Yasumoto K, Tatano Y, Shimizu T, Tomioka H
    • Journal Title

      Shimane Journal of Medical Science 27

      Pages: 17-24

    • Peer Reviewed
  • [Journal Article] Properties of two-type subpopulations of immunosuppressive macrophages generated by Mycobacterium intracellular infection.2009

    • Author(s)
      Tatano Y, Shimizu T, Tomioka H.
    • Journal Title

      Shimane J Med Sci 26

      Pages: 1-7

    • Peer Reviewed
  • [Journal Article] Experimental and clinical studies on rifacunna -The new effective antituberculous drug-.2009

    • Author(s)
      Dimova V, Tomioka H, Sano C, et al.
    • Journal Title

      Recent Patents on Anti-Infect Drug Discov 5

      Pages: 76-90

    • Peer Reviewed
  • [Journal Article] Recent advances in antituberculous drug development and novel drug targets.2008

    • Author(s)
      Tomioka H, Tatano Y, Yasumoto K, Shimizu T
    • Journal Title

      Expert Rev Respir Med 2

      Pages: 455-471

    • Peer Reviewed
  • [Journal Article] Development of new antituberculous agents based on new drug targets and structure-activity relationship.2008

    • Author(s)
      Tomioka H.
    • Journal Title

      Expert Opin Drug Discov 3

      Pages: 1-29

    • Peer Reviewed
  • [Presentation] 【教育講演】抗結核薬開発の現況と雇望-新しいdrug targetの探索2010

    • Author(s)
      冨岡治明
    • Organizer
      第85回日本結核病学会総会
    • Place of Presentation
      京都
    • Year and Date
      2010-05-21
  • [Book] Prospects for the development of new antituberculous drugs based on novel targets related to the host-parasite relationship in tuberculosis. (In : Emerging Trends in Antibacterial Discovery.)(A.A.Miller, P.F.Miller (ed.))2011

    • Author(s)
      Tomioka H
    • Total Pages
      241-279
    • Publisher
      Scientific Press, Norwich, UK, (in press)
  • [Book] Immunology of Leprosy - Roles of Cytokines in Host Defense against Leprosy Bacilli.(In : Leprosy)(M.Makino, et al (ed.))2011

    • Author(s)
      Tomioka H
    • Total Pages
      72-88
    • Publisher
      Tokai University Press, Kanagawa, Japan.
  • [Book] Suppressor macrophages induced by mycobacterial infections.(In : Current Topics on the Profiles of Host Immunological Response to Mycobacterial Infections, Research Signpost, p.251-280)(H. Tomioka (ed.))2009

    • Author(s)
      Tomioka H
    • Total Pages
      251-280
    • Publisher
      Kerala, India

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi