2010 Fiscal Year Final Research Report
Mucosa-associated bacteria in ulcerative colitis before and after new antibiotic combination therapy : DNA polymorphism analysis
| Project/Area Number |
20591209
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Infectious disease medicine
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
OHKUSA Toshifumi Jikei University School of Medicine, 医学部, 教授 (50160445)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KOIDO Shigeo 東京慈恵会医科大学, 医学部, 准教授 (70266617)
ODAHARA Shunichi 東京慈恵会医科大学, 医学部, 助教 (50445673)
KOMITA HIDEO 東京慈恵会医科大学, 医学部, 助教 (90534561)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 潰瘍性大腸炎 / 粘膜細菌フローラ / 細菌DNA / T-RFLP法 / メタゲノム解析 |
|---|
| Research Abstract |
We examined the efficacy of three-antibiotics combination therapy against F. varium, and investigated the mucosa-associated bacteria before and after the therapy using a molecular approach. Twenty patients with ulcerative colitis were randomly assigned into the antibiotic treatment group (amoxicillin, tetracycline, and metronidazole for 2 weeks) of 12 patients and placebo group of 8 patients. Colonoscopic biopsy was performed at 0, 3 and 12 months after the treatment. DNA from mucosal bacteria was isolated from biopsy specimens. We investigated the mucosa-associated bacterial components by terminal restriction fragment length polymorphism (T-RFLP) with the restriction enzyme HhaI, and quantified the change in the number of Fusobacterium varium by real-time PCR. After the treatment, profile changes of T-RFLP were found after treatment in 10 of 12 patients in the antibiotic group and none of 8 patients in the placebo group (p=0.0007). Based on the real time PCR study, F. varium/actin ratio was reduced after treatment in 8 of 12 patients (67%) in the antibiotic group and 4 of 8patients (50%) in the placebo group. Mucosal RNA extracts were also analyzed by Nextera RNA sequencing system. The number of Bacteroides, Acidaminococcus, Prevotella, Parabacteroides were decreased in the antibiotic group. Some number of mucosa-associated bacteria significantly decreased after the antibiotics combination therapy.
|
-
-
-
-
-
-
-
-
-
[Journal Article] Newly Developed Antibiotic Combination Therapy for Ulcerative Colitis : A Double-Blind Placebo-Controlled Multicenter Trial.2010
Author(s)
Ohkusa T, Kato K, Terao S, Chiba T, Mabe K, Murakami K, Mizokami Y, Sugiyama T, Yanaka A, Takeuchi Y, Yamato S, Yokoyama T, Okayasu I, Watanabe S, Tajiri H, Sato N
-
Journal Title
Am J Gastroenterol. 105(8)
Pages: 1820-9
-
-
-
-
-
-
-
-
-
-
[Journal Article] Correlations among total colonoscopic findings, clinical symptoms, and laboratory markers in ulcerative colitis.2008
Author(s)
Osada T, Ohkusa T, Okayasu I, Yoshida T, Hirai S, Beppu K, Shibuya T, Sakamoto N, Kobayashi O, Nagahara A, Terai T, Watanabe S
-
Journal Title
J Gastroenterol Hepatol. 23 Suppl 2
Pages: S262-7
-
-
-
-
-
-
-
[Presentation] Seasonal varidations in onset and relapse of ulcerative colitis in Japan.2009
Author(s)
Saito H, Ohkusa T, Yokoyama T, Harada A, Naoe K, Beppu K, Shibuya T, Sakamoto N, Osada T, Nagahara A, Watanabe S
Organizer
The 13th Taishotoyama International Symposium on Gastroenterology.
Year and Date
20090417-20090418
-
[Presentation] Factors associated with incomplete total colonoscopy in a university hospital in Japan.2009
Author(s)
Naoe K, Ohkusa T, Yokoyama T, Harada A, Saitou H, Beppu K, Shibuya T, Sakamoto N, Osada T, Nagahara A, Watanabe S
Organizer
The 13th Taishotoyama International Symposium on Gastroenterology.
Year and Date
20090417-20090418
-
-
-
-
-
-
[Presentation] 潰瘍性大腸炎(UC)の臨床症状およびinter-observer agreementからみた新たな内視鏡診断スコアの試み2008
Author(s)
長田太郎,大草敏史,澁谷智義,坂本直人,玉城久美子,笠原桂子,森広樹,斉藤紘昭,大高道郎,永原章仁,荻原達雄,渡辺純夫
Organizer
第76回日本消化器内視鏡学会総会
Place of Presentation
東京
Year and Date
20081001-20081004
-
-
[Presentation] Interobserver and Intra-observer consistency in the endoscopic agreement of ulcerative colitis activities using various classification.2008
Author(s)
Osada T, Ohkusa T, Yokoyama T, Shibuya T, Sakamoto N, Beppu K, Shimada Y, Takada M, Tamaki K, Nagahara A, Ogihara T, Watanabe S
Organizer
Digestive Disease Week
Place of Presentation
San Diego, USA
Year and Date
20080000
-
[Presentation] ANTIBIOTIC-INDUCED ULCERATIVE COLITIS REMISSIONS : A DOUBLE-BLIND PLACEBO-CONTROLLED MULTICENTRE TRIAL.2008
Author(s)
Ohkusa T, Kato K, Terao S, Chiba T, Mabe K, Murakami K, Mizokami Y, Sugiyama T, Kudo T, Yanaka A, Takeuchi Y, Yamato S, Yokoyama T, Okayasu I, Sato N, Watanabe S, Tajiri H
Organizer
16th United European Gastroenterology Week (UEGW) Vienna 2008
Place of Presentation
Vienna, Austria
Year and Date
2008-10-21
-
-
-
