2010 Fiscal Year Final Research Report
Reproduction islet transplantation for youth onset I type diabetes The use of the tissue engineering
| Project/Area Number |
20591227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
YAMASHITA Michitoshi Fukushima Medical University, 医学部, 助教 (20381387)
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| Co-Investigator(Kenkyū-buntansha) |
ISE Kazuya 福島県立医科大学, 医学部, 講師 (90363746)
SATOH Yoshihiro 福島県立医科大学, 医学部, 博士研究員 (60347218)
ISHII Shou 福島県立医科大学, 医学部, 助教 (40468129)
MATSUYAMA Shinichi 福島県立医科大学, 医学部, 博士研究員 (90381384)
GOTOH Mitsukazu 福島県立医科大学, 医学部, 教授 (50162160)
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| Project Period (FY) |
2008 – 2010
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| Keywords | I型糖尿病 / 膵島移植 / 膵島再生 / 細胞シート / ティッシュエンジニアリング |
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| Research Abstract |
After reaching confluency on temperature-responsive culture dishes, cultured rat islet cells were harvested as a uniformly connected tissue sheet by lowering culture temperature. Histological examination showed that harvested cell sheet had a monolayered 2-D structure, which established and retained its structural cell-to-cell connections. Immunohistological staining revealed that islet cell sheet comprised of insulin- and glucagon- staining positive cells. By transplanting islet cell sheets into the subcutaneous space of mice, islet tissues were successfully engineered and sustained. Therapeutic effectiveness of this islet bioengineering procedure was also confirmed in the transplantation study to induced diabetic mice.
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[Journal Article] Actions of the Japanese Pancreas and Islet Transplantation Association Regarding Transplanted Human Islets Isolated Using Liberase HI.2010
Author(s)
Saito T, Anazawa T, Gotoh M, Uemoto S, Kenmochi T, Kuroda Y, Satomi S, Itoh T, Yasunami Y, Kitamoto T, Mohri S, Teraoka S
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Journal Title
Transplantation Proceedings 42巻
Pages: 4213-4216
Peer Reviewed
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