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2010 Fiscal Year Final Research Report

Analysis of the mechanism of infantile allergic diseases using the system to differentiate human ES cells into immune cells

Research Project

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Project/Area Number 20591244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionThe University of Tokyo

Principal Investigator

MA Hou  The University of Tokyo, 医科学研究所, 特任研究員 (20378748)

Co-Investigator(Kenkyū-buntansha) TSUJI Kohichiro  東京大学, 医科学研究所, 准教授 (50179991)
Project Period (FY) 2008 – 2010
Keywordsヒト胚性幹細胞(ES細胞) / 乳児アレルギー疾患 / 肥満細胞 / 好酸球
Research Abstract

We succeeded the production of human mast cells and eosinophils, major immune effecter cells in allergic diseases, from hematopoietic cells derived human ES cells by coculture with AGMs-3 cells which are stromal cell line established from murine embryonic AGM region. Since this system reflect the generation and development of these immune cells in human embryo, it is expected to be a useful tool for analysis of the mechanism causing infantile allergic diseases.

  • Research Products

    (43 results)

All 2011 2010 2009 2008 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (24 results) Book (4 results) Remarks (2 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Sero-prevalence of pandemic (H1N1) 2009 influenza A virus among schoolchildren and their parents inTokyo, Japan.2011

    • Author(s)
      Iwatsuki-Horimoto K, Horimoto T, Tamura D, Kiso M, Kawakami E, Hatakeyama S, Ebihara Y, Koibuchi T, Fujii T, Takahashi K, Shimojima M, Sakai-Tagawa Y, Ito M, Sakabe S, Iwasa A, Takahashi K, Ishii T, Gorai T, Tsuji K, Iwamoto A, Kawaoka Y
    • Journal Title

      J Clinic Microbiol (掲載確定)

    • Peer Reviewed
  • [Journal Article] Reduced dose chemotherapy for acute promyelocytic leukemia with adult Down syndrome.2011

    • Author(s)
      Tsuda M, Ebihara Y, Mochizuki S, Uchimaru K, Tojo A, Tsuji K
    • Journal Title

      Br J Haematol (掲載確定)

    • Peer Reviewed
  • [Journal Article] ヒトES細胞から血液細胞への分化誘導2011

    • Author(s)
      辻浩一郎
    • Journal Title

      Cytometry Research (掲載確定)

    • Peer Reviewed
  • [Journal Article] Adhesion-mediated self-renewal abilities of Ph+ blastoma cells.2010

    • Author(s)
      Funayama K, Saito-Kurimoto Y, Ebihara Y, Shimane Y, Nomura H, Tsuji K, Asano S
    • Journal Title

      Biochem Biophys Res Commun 396

      Pages: 193-198

    • Peer Reviewed
  • [Journal Article] A 5' untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model.2010

    • Author(s)
      Nagamachi A, Htun PW, Ma F, Miyazaki K, Yamasaki N, Kanno M, Inaba T, Honda Z, Okuda T, Oda H, Tsuji K, Honda H H
    • Journal Title

      Dev Biol 345

      Pages: 226-236

    • Peer Reviewed
  • [Journal Article] Homeostatic erythropoiesis by the transcription factor IRF2 through attenuation of type I interferon signaling.2008

    • Author(s)
      Mizutani T, Mizutani T, Tsuji K, Ebihara Y, Ohba Y, Taniguchi T, Honda K
    • Journal Title

      Exp Hematol 36

      Pages: 255-264

    • Peer Reviewed
  • [Journal Article] Cardiovascular toxicity of cryopreserved cord blood cell infusion.2008

    • Author(s)
      Konuma T, Konuma T, Ooi J, Takahashi S, Tomonari A, Tsukada N, Kobayashi T, Sato A, Kato S, Kasahara S, Ebihara Y, Nagamura-Inoue T, Tsuji K, Tojo A, Asano S
    • Journal Title

      Bone Marrrow Transplant 41

      Pages: 861-865

    • Peer Reviewed
  • [Journal Article] Direct development of functionally mature tryptase/chymase double positive connective tissue-type mast cells from primate ES cells.2008

    • Author(s)
      Ma F, Kambe N, Wang D, Shinoda G, Fujino H, Umeda K, Fujisawa A, Ma L, Suemori H, Nakatsuji N, Miyachi Y, Torii R, Tsuji K, Heike T, Nakahata T
    • Journal Title

      Stem Cells 26(3)

      Pages: 706-714

    • Peer Reviewed
  • [Journal Article] Generation of functional erythroid cells from human embryonic stem cell-derived definitive hematopoiesis.2008

    • Author(s)
      Ma F, Ebihara Y, Umeda K, Sakai H, Hanada S, Zhang H, Tsuchida E, Nakahata T, Nakauchi H, Tsuji K
    • Journal Title

      Proc Natl Acad Sci USA 105(35)

      Pages: 13087-13092

    • Peer Reviewed
  • [Presentation] ヒトiPS細胞から血液細胞への動物血清・細胞非依存的分化誘導:自己iPS細胞由来 間葉系幹細胞との共培養2011

    • Author(s)
      海老原康博, 他
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      東京:京王プラザホテル
    • Year and Date
      20110301-20110302
  • [Presentation] 15-20歳の思春期症例に対する臍帯血移植の検討2010

    • Author(s)
      海老原康博, 他
    • Organizer
      第52回日本小児血液学会総会
    • Place of Presentation
      大阪:大阪国際会議場
    • Year and Date
      20101217-20101219
  • [Presentation] Generation of functionally mature blood cells from human ES/iPS cells and their possible clinical utilization.2010

    • Author(s)
      Ma F.
    • Organizer
      BIT's 3^<rd> Annual World Congress of RegenerativeMedicine & Stem Cells (RMSC-2010)
    • Place of Presentation
      Shanghai Everbright Convention and Exhibition Center Shanghai, China
    • Year and Date
      20101205-20101207
  • [Presentation] Differentiation of mature eosinophils derived from human embryonic and induced pluripotent stem cells.2010

    • Author(s)
      Yang W, et al.
    • Organizer
      52^<nd> ASH Annual Meeting
    • Place of Presentation
      Orange County Convention Center, Orlando, Florida, USA
    • Year and Date
      20101204-20101207
  • [Presentation] Hematopoiesis of human induced pluripotent stem cells derived from patients with Down syndrome.2010

    • Author(s)
      Nishihama N, et al.
    • Organizer
      52^<nd> ASH Annual Meeting
    • Place of Presentation
      Orange County Convention Center, Orlando, Florida, USA
    • Year and Date
      20101204-20101207
  • [Presentation] Generation of functionally mature blood cells from human pluripotent stem cells.2010

    • Author(s)
      Ma F.
    • Organizer
      International Forum on Stem Cells 2010
    • Place of Presentation
      Chinese Academy of Medical Sciences Tianjin, China
    • Year and Date
      20101111-20101113
  • [Presentation] The novel transfusion and cellular therapies based on human ES and iPScells.2010

    • Author(s)
      Ma F.
    • Organizer
      5^<th> National Congress of the China Society of Blood Transfusion
    • Place of Presentation
      The Century City New International Convention & Exhibition Center, Chengdu, China
    • Year and Date
      20101104-20101107
  • [Presentation] The impact of steroid use as a GVHD treatment or prophylaxis within100 days after CBT.2010

    • Author(s)
      Kawakita T, et al.
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      神奈川:パシフィコ横浜
    • Year and Date
      20100924-20100926
  • [Presentation] Acute promyelocytic leukemia with adult Down syndrome : a case report.2010

    • Author(s)
      Tsuda M, et al.
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      神奈川:パシフィコ横浜
    • Year and Date
      20100924-20100926
  • [Presentation] Generation of mature eosinophils from human embryonic and induced pluripotent stem cells.2010

    • Author(s)
      Ma F, et al.
    • Organizer
      39^<th> Annual Scientific Meeting of ISEH
    • Place of Presentation
      Melbourne Convention & Exhibition Centre, Melbourne Australia
    • Year and Date
      20100815-20100818
  • [Presentation] ヒトES細胞から血液細胞への分化誘導2010

    • Author(s)
      辻浩一郎
    • Organizer
      第20回日本サイトメトリー学会学術集会
    • Place of Presentation
      東京:東京慈恵会医科大学
    • Year and Date
      20100626-20100627
  • [Presentation] 慢性骨髄性白血病に対する骨髄破壊的前処置を用いた臍帯血移植2010

    • Author(s)
      河北敏郎, 他
    • Organizer
      第32回日本造血細胞移植学会
    • Place of Presentation
      静岡:アクトシティ浜松
    • Year and Date
      20100219-20100220
  • [Presentation] 再発・難治性白血病4例に対するIDA-FLAG療法の効果および有効性2009

    • Author(s)
      渡辺温子, 他
    • Organizer
      第51回日本小児血液学会
    • Place of Presentation
      千葉:東京ベイホテル東急
    • Year and Date
      20091127-20091129
  • [Presentation] Second or third myeloablative allo-SCT using cord blood for adult leukemia.2009

    • Author(s)
      Kato S, et al
    • Organizer
      第71回日本血液学会総会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      20091023-20091025
  • [Presentation] Human ES cell-derived MSC maintining human ES and iPS cells under animal serum-free conditions.2009

    • Author(s)
      Ebihara Y, et al
    • Organizer
      第71回日本血液学会総会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      20091023-20091025
  • [Presentation] Derivation of mature blood cells from human induced pluripotent stem cells.2009

    • Author(s)
      Ma F, et al
    • Organizer
      38th Annual Scientific Meeting of the International Society of Experimental Hematology
    • Place of Presentation
      Horel Divani Caravel, Athens, Greece
    • Year and Date
      20090909-20090912
  • [Presentation] Human embryonic stem (ES) cell-derived mesenchymal stem cells capable of efficiently maintaining human ES and induced pluripotent stem cells under animal serum-free conditions.2009

    • Author(s)
      Ebihara Y, et al
    • Organizer
      7th Annual Meeting of the International Society of Stem Cell Research
    • Place of Presentation
      Centre convencions Internacional, Barcelona, Spain
    • Year and Date
      20090708-20090711
  • [Presentation] Derivation of multipotential hematopoietic progenitors and mature blood cells from human induced pluripotent stem cells.2009

    • Author(s)
      Ma F, et al
    • Organizer
      7th Keio Stem Cell Research Symposium
    • Place of Presentation
      東京:慶応義塾大学
    • Year and Date
      20090515-20090516
  • [Presentation] 15-20歳の思春期症例に対する臍帯血移植の検討2009

    • Author(s)
      海老原康博, 他
    • Organizer
      第112回日本小児科学会学術集会
    • Place of Presentation
      奈良:奈良県文化会館
    • Year and Date
      20090417-20090419
  • [Presentation] Regenerative medicine and hematopoietic stem cells.2009

    • Author(s)
      辻浩一郎
    • Organizer
      Educational Symposium for Current Pediatric Hematology
    • Place of Presentation
      中国医科学研究院/天津血液研究所病院 Tianjin, China
    • Year and Date
      2009-09-18
  • [Presentation] ヒトES細胞維持機能を有するヒトES細胞由来ストローマ細胞2009

    • Author(s)
      海老原康博, 他
    • Organizer
      第8回日本再生医療学会
    • Place of Presentation
      東京:東京国際フォーラム
    • Year and Date
      2009-03-05
  • [Presentation] 15-20歳の思春期奨励に対する臍帯血移植の検討2009

    • Author(s)
      海老原康博, 他
    • Place of Presentation
      北海道:ロイトン札幌
    • Year and Date
      2009-02-05
  • [Presentation] Myelodysplastic Syndrome (MDS) and Myeloproliferative Disease (MPD) in Children : A prospective Resistration of 222 Cases.2008

    • Author(s)
      Hirabayashi S, et al.
    • Organizer
      15th annual meeting of American Society of Hematology
    • Place of Presentation
      Moscone Convention Center, San Francisco, USA
    • Year and Date
      2008-12-07
  • [Presentation] 異種動物血清を用いないヒトembryonic stem cell(hESC)から間葉系幹細胞(MSC)への分化誘導2008

    • Author(s)
      海老原康博, 他
    • Organizer
      第70回日本血液学会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      2008-10-10
  • [Book] Stem Cell Therapy2011

    • Author(s)
      Ma F, Yang W, Tsuji K
    • Total Pages
      51-70
    • Publisher
      Transworld Research Network, Kerala,
  • [Book] Embryonic stem cells2011

    • Author(s)
      Ma F, Ebihara Y, Tsuji K
    • Publisher
      Intech, Vienna(掲載確定)
  • [Book] Lineage-specific differentiation of human embryonic and induced pluripotent stem cells methods and protocols2011

    • Author(s)
      Ma F, Gu Y, Nishihama N, Ebihara Y, Tsuji K
    • Publisher
      Humana Press, New York(掲載確定)
  • [Book] 臍帯血移植の基礎と臨床2011

    • Author(s)
      辻浩一郎
    • Publisher
      医学書院(東京)(掲載確定)
  • [Remarks] ホームページ等

  • [Remarks] 東京大学医科学研究所 幹細胞治療研究センター 幹細胞プロセシング分野

    • URL

      http://stemcell-u.tokyo.org/scp/

  • [Patent(Industrial Property Rights)] Method for producing eosinophils from pluripotent stem cells2010

    • Inventor(s)
      辻浩一郎、馬峰、斎藤博久、松本健治、中畑龍俊
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許,61/419,496
    • Filing Date
      2010-12-03
  • [Patent(Industrial Property Rights)] ヒト由来の多能性幹細胞を分化させる方法2010

    • Inventor(s)
      辻浩一郎、海老原康博
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Number
      特許,特願2010-222583
    • Filing Date
      2010-09-30
  • [Patent(Industrial Property Rights)] Method for producing mast cells from pluripotent stem cells2010

    • Inventor(s)
      辻浩一郎、馬峰、中畑龍俊
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許,PCT/JP2010/065893
    • Filing Date
      2010-09-08
  • [Patent(Industrial Property Rights)] 多能性幹細胞からの肥満細胞の製造方法2009

    • Inventor(s)
      辻浩一郎、馬峰、中畑龍俊
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許,61/240,376
    • Filing Date
      2009-09-08

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Published: 2012-01-26   Modified: 2016-04-21  

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