2010 Fiscal Year Final Research Report
Gene expression of hematopoietic transcription factors in pediatric acute megakaryocytic leukemia
| Project/Area Number |
20591251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nihon University |
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Principal Investigator |
YAGASAKI Hiroshi Nihon University, 医学部, 助教 (90378141)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Seiji 名古屋大学, 大学院・医学系研究科, 教授 (20313992)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 急性巨核芽球性白血病 / 転写因子 / ヒエラルキー |
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| Research Abstract |
Acute megakaryoblastic leukemia (AMKL) has a diverse heterogeneity of the disease including morphology and cytogenetic studies. In children, 2 major subgroups have been described : AMKL developing in patients with Down syndrome (DS-AMKL) and AMKL in patients without Down syndrome (non-DS-AMKL). To compare the biological characteristics of blast cells with DS-AMKL and non-DS-AMKL, we compared the expression of hematopoietic transcription factors in blast cells derived from 19 children with AMKL (DS-AMKL : 11, non-DS-AMKL : 9). The morphology of blast cells was categorized into four groups : type 1, completely undifferentiated blasts ; type 2, intermediately differentiated blasts with cytoplasmic blebs ; type 3, blasts with dysmegakaryocytopoiesis ; type 4, blasts with deep blue cytoplasm. To define the differentiation stage by molecular level, we quantified the expression of transcription factors related to megakaryo-erythroid cell lineages ; GATA1, GATA2, FOG-1, NF-E2, SCL, PU.1, C/EBPα, c-mpl, Epo-R, CD41b, βglobin. In conclusion, blasts of DS-AMKL differentiated into not only megakaryocyte but also erythroid lineage because of overexpression of glycophorin A and βglobin under the GATA1 gene mutations. DS-AMKL should be classified into other categories apart from non-DS-AMKL considering the totally different patterns in gene alterations and levels of transcription factors.
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[Journal Article] Comparison of matched-sibling donor BMT and unrelated donor BMT in children and adolescent with acquired severe aplastic anemia.2010
Author(s)
Yagasaki H, Takahashi Y, Hama A, Kudo K, Nishio N, Muramatsu H, Tanaka M, Yoshida N, Matsumoto K, Watanabe N, Kato K, Horibe K, Kojima S
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Journal Title
Bone Marrow Transplant. 45(10)
Pages: 1508-1513
Peer Reviewed
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[Journal Article] Tacrolimus/Methotrexate versus cyclosporine/methotrexate as graft-versus-host disease prophylaxis in patients with severe aplastic anemia who received bone marrow transplantation from unrelated donors : results of matched pair analysis.2009
Author(s)
Yagasaki H, Kojima S, Yabe H, Kato K, Kigasawa H, Sakamaki H, Tsuchida M, Kato S, Kawase T, Muramatsu H, Morishima Y, Kodera Y
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Journal Title
Biol Blood Marrow Transplant. 15(12)
Pages: 1603-1608
Peer Reviewed
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