2010 Fiscal Year Final Research Report
Investigation of Molecular Mechanisms of Apoptosis using gene knock-out leukemic cell lines
| Project/Area Number |
20591261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kitasato University |
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Principal Investigator |
MIYASHITA Toshiyuki Kitasato University, 医学部, 教授 (60174182)
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| Project Period (FY) |
2008 – 2010
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| Keywords | アポトーシス / グルココルチコイド / RCAN1 / 白血病 |
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| Research Abstract |
In order to investigate the molecular mechanism of how glucocorticoid (GC) induces apoptosis in certain leukemic cells, we established RCAN1 gene knock-out Nalm6 cells. RCAN1 is one of the target genes of GC and Nalm6 is a human leukemic cell line that undergoes apoptosis upon the treatment with GC. RCAN1 -/- cells were markedly resistant to GC compared with wild-type cells. On the contrary, overexpression of RCAN1 resulted in enhanced apoptosis by GC. These resultsdemonstrate that RCAN1 is a mediator for GC-induced apoptosis in leukemic cells.
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[Journal Article] EYA4, Deleted in a case with middle interhemispheric variant of holoprosencephaly, interacts with SIX3 both physically and functionally.2009
Author(s)
Abe Y., Oka A., Mizuguchi M., Igarashi T., Ishikawa S., Aburatani H., Yokoyama S., Asahara H., Nagao K., Yamada M., Miyashita T.
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Journal Title
Hum.Mutat. 30
Pages: E946-E955
Peer Reviewed
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[Journal Article] Patched1 haploinsufficiency increases adult bone mass and modulates Gli3 repressor activity.2008
Author(s)
Ohba S., Kawaguchi H., Kugimiya F., Ogasawara T., Kawamura N., Saito T., Ikeda T., Fujii K., Miyajima T., Kuramochi A., Miyashita T., Oda H., Nakamura K., Takato T., Chung, U.I.
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Journal Title
Dev.Cell 14
Pages: 689-699
Peer Reviewed
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[Presentation]2010
Author(s)
宮下俊之,遠藤真美子,齋藤加代子,杉田克生,藤井克則
Organizer
第16回日本家族性腫瘍学会学術集会
Place of Presentation
新潟
Year and Date
20100709-20100710
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