2010 Fiscal Year Final Research Report
Role of neural cell adhesion molecules in structural and functional remodeling of fetal adrenal glands
| Project/Area Number |
20591305
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIMOTO Hitoshi 東海大学, 医学部, 教授 (10212937)
ASAI Satoshi 慶應義塾大学, 医学部, 助教 (70383867)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 胎児副腎 / NCAM / Syndecan-3 |
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| Research Abstract |
We examined expression and localization of NCAM and SDC3 in the HFA, and investigated whether NCAM and SDC3 are implicated in HFA zonal development. Immunoreactive NCAM and SDC3 were limited to the DZ. LCM/qPCR showed a similar zonal differential SDC3 expression at the mRNA level. SDC3 knockdown attenuated FGF2-induced cell proliferation while increased cAMP-induced DHEAS production and mRNA levels of FZ-cell markers. Addition of ACTH or FGF2 did not change SDC3 mRNA in isolated DZ and NCI-H295A cells. These results suggest that NCAM and SDC3 may play a role in development of functional zonation of the HFA, at least partly through maintaining some aspects of the DZ phenotype.
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