2010 Fiscal Year Final Research Report
Development of PCR-based whole-genome analysis method and the application for the prenatal diagnosis from maternal blood
| Project/Area Number |
20591308
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Showa University |
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Principal Investigator |
MATSUOKA Ryu Showa University, 医学部, 講師 (20349111)
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| Co-Investigator(Kenkyū-buntansha) |
SEKIZAWA Akihiko 昭和大学, 医学部, 准教授 (10245839)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 母体血 / 有核赤血球 / 無侵襲 / 出生前診断 / ダウン症候群 |
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| Research Abstract |
Non-invasive prenatal diagnosis through examination of intact fetal cells circulating within maternal blood can be used to diagnose a full range of genetic disorders. Since only a limited number of fetal cells circulate within maternal blood, procedures to enrich the cells and enable single cell analysis with high sensitivity are required. Recently, we have developed the separation methods, including a lectin-based method and autoimage analyzing, leading to the improved sensitivity of genetic analysis. We recently proved the accuracy of the prenatal diagnosis of trisomy-21 and -18 by the nucleated erythrocytes analysis in maternal blood. Thus, this progress has supported the possibility of non-invasive prenatal diagnosis of genetic disorders.
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