Regenerative medical therapy using bone marrow derived cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591332
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hokkaido University
• Principal Investigator: ABE Riichiro Hokkaido University, 大学院・医学研究科, 准教授 (60344511)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Hiroshi 北海道大学, 大学院・医学研究科, 教授 (00146672)
• Project Period (FY): 2008 – 2010
• Keywords: 皮膚炎症 / 再生学

Research Abstract

Recent studies have suggested that bone marrow cells transdifferentiate to a variety of cellular lineages including skin. Investments for regenerative medicine have been used respective model mouse as a preclinical step. In our study, we conducted basic research to achieve radical cure for epidermolysis bullosa to which there is no effective therapy.
First as a basic research step, we demonstrated the existence of the factors secreted from keratinocytes that induces transdifferentiation from bone marrow derived cells into keratinocyte. Next as a preclinical step, bone marrow transplantation from normal mouse to type XVII collagen knock out mouse, one of the epidermolysis bullosa model mouse, induced production of the defect protein (XVII type collagen) and improvement of clinical manifestation of the mouse. So we suggested that the bone marrow transplantation from normal mouse to model mouse can be one of the effective therapies for epidermolysis bullosa..

Research Products

• [Journal Article] Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice. (2010)
  • Author(s): Fujita Y, Abe R, Inokuma D, Sasaki M, Hoshina D, Natsuga K, Nishie W, McMillan JR, Nakamura H, Shimizu T, Akiyama M, Sawamura D, Shimizu H.
  • Journal Title: Proc Natl Acad Sci USA 107 Pages: 14345-50
  • Peer Reviewed
• [Journal Article] Prevalent LIPH founder mutations lead to loss of P2Y5 activation ability of PA-PLA1alpha in autosomal recessive hypotrichosis. (2010)
  • Author(s): Shinkuma S, Akiyama M, Inoue A, Aoki J, Natsuga K, Nomura T, Arita K, Abe R, Ito K, Nakamura H, Ujiie H, Shibaki A, Suga H, Tsunemi Y, Nishie W, Shimizu H.
  • Journal Title: Hum Mutat 31 Pages: 602-10
  • Peer Reviewed
• [Journal Article] Collagen XVII participates in keratinocyte adhesion to collagen IV, and in p38MAPK-dependent migration and cell signaling. (2009)
  • Author(s): Qiao H, Shibaki A, Long HA, Wang G, Li Q, Nishie W, Abe R, Akiyama M, Shimizu H, McMillan JR.
  • Journal Title: J Invest Dermatol 129 Pages: 2288-95
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells are recruited into wounded skin and contribute to wound repair by transdifferentiation into multiple skin cell type. (2008)
  • Author(s): Sasaki M, Abe R, Fujita Y, Ando S, Inokuma D, Shimizu H.
  • Journal Title: J Immunol 180 Pages: 2581-7
  • Peer Reviewed
• [Presentation] 骨髄細胞を用いた表皮水疱症治療 (2010)
  • Author(s): 阿部理一郎
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-17
• [Remarks] ホームページ等
• [Remarks] 北海道大学医学部皮膚科ホームページ
  • URL: http://www.derm-hokudai.jp/jp/