Control mechanism of melanogenesis by pigmentation-related genes and ultraviolet radiation

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Fujita Health University
• Principal Investigator: ITO Shosuke Fujita Health University, 藤田保健衛生大学, 名誉教授 (70121431)
• Co-Investigator(Kenkyū-buntansha): WAKAMATSU Kazumasa 藤田保健衛生大学, 医療科学部, 教授 (80131259)
• Co-Investigator(Renkei-kenkyūsha): HIROBE Tomohisa 放射線医学総合研究所, 生体影響機構研究, グループ・チームリーダー (10111238)
• Project Period (FY): 2008 – 2010
• Keywords: メラニン / 遺伝子 / 紫外線

Research Abstract

Our present results suggest that melanoblast/melanocyte proliferation and melanocyte differentiation are regulated in a time-and development-dependent manner by many coat color genes, especially Agouti (A), brown (Tyrp1^b/b), albino (Tyr^c/c), dilute (Myo5a^d/d), recessive yellow (Mc1r^e/e), pink-eyed dilution (Oca2^p/p), slaty (Dct^slt/slt) and ruby-eye 2^d(ru2^d/Hps5^ru2-d) otherwise known for their ability to regulate melanosome formation and maturation, pigment switching, melanosome distribution and transfer.

Research Products

• [Journal Article] Usefullness of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin in various tissue samples : Application to chemical analysis of human hair melanins (2011)
  • Author(s): Ito S, Nakanishi Y, Valenzuela RK, Brilliant MH, Ludger K, Wakamatsu K
  • Journal Title: Pigment Cell Melanoma Res 24(in press)
  • Peer Reviewed
• [Journal Article] A novel deletion mutation of mouse ruby-eye 2 named ru2^d/Hps^5ru^<2-d> inhibits melanocyte differentiation and its impaired differentiation is rescued by L-tyrosine (2011)
  • Author(s): Hirobe T, Yoshihara C, Takeuchi S, Wakamastu K, Ito S, Abe H, Kawa Y, Soma Y
  • Journal Title: Zool Sci 28(in press)
  • Peer Reviewed
• [Journal Article] The mouse pink-eyed dilution allele of the P-gene greatly inhibits eumelanin, but not pheomelanin synthesis as studied by chemical analysis (2011)
  • Author(s): Hirobe T, Ito S, Wakamatsu K
  • Journal Title: Pigment Cell Melanoma Res 24 Pages: 241-246
  • Peer Reviewed
• [Journal Article] What we have learned and have not learned fro mouse coat color pigmentation (2011)
  • Author(s): Ito S, Wakamatsu K. Human hair melanins
  • Journal Title: Pigment Cell Melanoma Res 24(総説) Pages: 63-74
  • Peer Reviewed
• [Journal Article] Abdel-Malek ZA Melanocortin 1 receptor genotype : an important determinant of the damage response of melanocytes to ultraviolet radiation (2010)
  • Author(s): Kadekaro AL, Leachman S, Kavanagh RJ, Swope V, Cassidy P, Supp D, Sartor M, Schwemberger S, Babcock G, Wakamatsu K, Ito S, Koshoffer A, Boissy RE, Manga P, Sturm R
  • Journal Title: FASEB J 24 Pages: 380-390
  • Peer Reviewed
• [Journal Article] Protection against ultraviolet radiation involves MCR1-mediated non-pigmentary and pigmentary mechanisms in vivo (2010)
  • Author(s): Robinson S, Dixon S, August S, Diffey B, Wakamatsu K, Ito S, Friedmann P, Healy E
  • Journal Title: J Invest Dermatol 130 Pages: 1904-1913
  • Peer Reviewed
• [Journal Article] Estrogen increases hair pigmentation in female recessive yellow mice (2010)
  • Author(s): Hirobe T, Kiuchi M, Wakamatsu K, Ito S
  • Journal Title: Zool Sci 27 Pages: 470-476
  • Peer Reviewed
• [Journal Article] Predicting phenotype from genotype : normal pigmentation (2010)
  • Author(s): Valenzuela RK, Henderson MS, Walch MH, Garrison NA, Kelch JT, Cohen-Barak O, Erickson DT, Meaney FJ, Walsh JB, Cheng KC, Ito S, Wakamatsu K, Frudakis T, Thomas M, Brilliant MH
  • Journal Title: J Forensic Sci 55 Pages: 315-322
  • Peer Reviewed
• [Journal Article] Spiny mice modulate eumelanin to pheomelanin ratio to achieve cryptic coloration in "Evolution Canyon", Israel (2010)
  • Author(s): Singaravelen N, Pavlicek T, Beharav A, Wakamatsu K, Ito S, Nevo E
  • Journal Title: PLoS One 5 Pages: e8708(7)
  • Peer Reviewed
• [Journal Article] Current challenges in understanding melanogenesis : bridging chemistry, biological control, morphology, and function (2009)
  • Author(s): Simon JD, Peles D, Wakamatsu K, Ito S
  • Journal Title: Pigment Cell Melanoma Res 22(総説) Pages: 563-579
  • Peer Reviewed
• [Journal Article] Short- and long-term effects of UV on the pigmentation of human skin (2009)
  • Author(s): Coelho SG, Choi W, Brenner M, Miyamura Y, Yamaguchi Y, Wolber R, Smuda C, Kolbe L, Ito S, Wakamatsu K, Zmudzka B, Beer JZ, Miller SA, Hearing VJ
  • Journal Title: J Invest Dermatol Symp Proc 14 Pages: 31-35
  • Peer Reviewed
• [Journal Article] Stem cell factor rescues tyrosinase expression and pigmentation in discreet anatomic locations in albino mice (2009)
  • Author(s): Vanover JC, Spry ML, Hamilton L, Wakamatsu K, Ito S, D'Orazio JA
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 827-838
  • Peer Reviewed
• [Journal Article] Independent regulation of hair and skin color by two G protein-coupled pathways (2009)
  • Author(s): Van Raamsdonk, CD, Barsh GS, Wakamatsu K, Ito S
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 819-826
  • Peer Reviewed
• [Journal Article] Agouti protein, mahogunin, and attractin in pheomelanogenesis and melanoblast-like alteration of melanocytes : a cAMP-independent pathway (2009)
  • Author(s): Hida T, Wakamatsu K, Sviderskaya EV, Donkin AJ, Montoliu L, Lamoreux ML, Yu B, Millhauser GL, Ito S, Barsh GS, Jimbow K, Bennett DC
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 623-634
  • Peer Reviewed
• [Journal Article] Chemical analysis of late stages of pheomelanogenesis : conversion of dihydrobenzothiazine to a benzothiazole structure (2009)
  • Author(s): Wakamatsu K, Ohtara K, Ito S
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 474-486
  • Peer Reviewed
• [Journal Article] Comparison of eumelanin and pheomelanin content between cultured uveal melanoma cells and normal uveal melanocytes (2009)
  • Author(s): Hu D-N, Wakamatsu K, Ito S, McCormick SA
  • Journal Title: Melanoma Res 19 Pages: 75-79
  • Peer Reviewed
• [Journal Article] Prolonged treatment of fair-skinned mice with topical forskolin causes persistent tanning and UV protection (2009)
  • Author(s): Spry ML, Vanover JC, Scott T, Abona-Ama O, Wakamatsu K, Ito S, D'Orazio JA
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 219-229
  • Peer Reviewed
• [Journal Article] Pigmentation effects of solar-simulated radiation as compared with UVA and UVB radiation (2008)
  • Author(s): Wolber R, Schlenz K, Wakamatsu K, Smuda C, Nakanishi Y, Hearing VJ, Ito S
  • Journal Title: Pigment Cell Melanoma Res 21 Pages: 487-491
  • Peer Reviewed
• [Journal Article] Regulation of eumelanin/pheomelanin synthesis and visible pigmentation in melanocytes by ligands of the melanocortin 1 receptor (2008)
  • Author(s): Le Pape EL, Wakamatsu K, Ito S, Wolber R, Hearing VJ
  • Journal Title: Pigment Cell Melanoma Res 21 Pages: 477-486
  • Peer Reviewed
• [Journal Article] Characterization of melanin in human iridal and choroidal melanocytes from eyes with various colored irides (2008)
  • Author(s): Wakamatsu K, Hu D-N, McCormick SA, Ito S
  • Journal Title: Pigment Cell Melanoma Res 21 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Chemistry of mixed melanogenesis - pivotal roles of dopaquinone (2008)
  • Author(s): Ito S, Wakamatsu K
  • Journal Title: Photochem Photobiol 84(総説) Pages: 582-592
  • Peer Reviewed
• [Presentation] ルビーアイ2の新規突然遺伝子(ru^2d/Hps^<5d>)はマウスのメラノサイトの分化を抑制し毛色を薄くするが、L-チロシンはその分化活性を回復させる (2010)
  • Author(s): 広部知久
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京,日本
  • Year and Date: 2010-11-27
• [Presentation] Human hair pigmentation : Lessens from mouse hair pigmentation (2010)
  • Author(s): Ito S
  • Organizer: 4th Asian Society for Pigment Cell Research
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2010-06-14
• [Presentation] Estrogen increases the hair pigmentation in female recessive yellow mice (2010)
  • Author(s): 広部知久
  • Organizer: 4th Asian Society for Pigment Cell Research
  • Place of Presentation: Guanzhou, China
  • Year and Date: 2010-06-14
• [Presentation] フェオメラニン生成の後期過程の化学的分析 (2009)
  • Author(s): 若松一雅
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] Chemical and biochemical control of mixed melanogenesis (2009)
  • Author(s): Ito S
  • Organizer: 3rd Asian Society for Pigment Cell Research
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-06-11
• [Presentation] Reexamination of microanalytical methods for melanin assay (2008)
  • Author(s): 若松一雅
  • Organizer: Conjoint Meeting of XXth International Pigment Cell Conference & Vth International Melanoma Research Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-05-07
• [Presentation] Comparison of melanogenesis in cultured epidermal melanocytes from wild-type and mutant mice at the slaty locus (2008)
  • Author(s): Niki Y
  • Organizer: Conjoint Meeting of XXth International Pigment Cell Conference & Vth International Melanoma Research Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-05-07
• [Presentation] Agouti protein modifies melanocyte shape and melanogenesis through cAMP-independent signaling pathway (2008)
  • Author(s): Hida T
  • Organizer: Conjoint Meeting of XXth International Pigment Cell Conference & Vth International Melanoma Research Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-05-07
• [Presentation] Regulation of melanocytes fate by an agonist inverse of MC1R and agouti protein (2008)
  • Author(s): LePape E
  • Organizer: Conjoint Meeting of XXth International Pigment Cell Conference & Vth International Melanoma Research Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-05-07
• [Book] Melanin and Melanosomes(Riley PA, Borovansky J, Eds Structure of Melanins) (2011)
  • Author(s): Ito S, Wakamatsu K, d'Ischia M, Napolitano A, Pezzella A
  • Total Pages: 167-185
  • Publisher: Blackwell Publisher