Targeting of anticancer drugs using radiosensitive liquid-coremicrocapsules, via radiotherapy.

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20591503
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Iwate Medical University
• Principal Investigator: HARADA Satoshi Iwate Medical University (20244931)
• Project Period (FY): 2008 – 2012
• Keywords: 放射線生物学 / Drug Delivery System

Research Abstract

放射線により抗癌剤を放出するマイクロカプセルを、ヒアルロン酸とアルギン酸のFe^<2+>重合を用いて作成する。放射線による抗がん剤の放出機所は1.ヒアルロン酸による放射線の分解、2.アルギン酸のFe^<2+>重合の破綻。の2点である。
このマイクロカプセルを用いて、以下の2つの薬剤標的療法を研究する。
(1)皮下注射型マイクロカプセルによる薬剤標的療法:マイクロカプセルを腫瘍周囲の皮下組織に注射後、放射線を照射し、マイクロカプセルから放出された抗癌剤と放射線の相乗効果による抗腫瘍効果増強、薬剤限局化による抗癌剤副作用の軽減を計る。
(2)静脈注射型マイクロカプセルによる薬剤標的療法:第一段階として、マイクロカプセルを集積させたい腫瘍に、第1回目の放射線を照射、血管内皮細胞にP-selectin抗原を発現させる。その後、P-selectin抗体で標識したマイクロカプセルを静脈注射し、P-selectinの抗原抗体反応により、マイクロカプセルを第1回目照射部位に集積させる。最後に、集積させたマイクロカプセルに対し、第2回目の放射線を照射し、マイクロカプセルから抗癌剤を放出させる。マイクロカプセルから放出された抗癌剤と放射線の相乗効果による抗腫瘍効果増強、薬剤限局化による抗癌剤副作用の軽減を計る。

Research Products

• [Journal Article] Improved Radiosensitive Microcapsules Using H2O2 (2010)
  • Author(s): S.Harada, S.Ehara, K.Ishii, A.Tanaka, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, T.Sakai, K.Arakawa, M.Saitoh, S.Oikawa, K.Sera.
  • Journal Title: Int.J.PIXE 20 Pages: 30-36
• [Journal Article] Targeted Delivery of Chemotherapeutic Agents by using Improved Radiosensitive Liquid-Core Microcapsules and Assessment of Their Antitumor Effect. (2009)
  • Author(s): Harada, Satoshi, Ehara, Shigeru, Ishii, Keizoh, Yamazaki, Hiromichi, Matsuyama, Shigeo, Sakai, Takuro, Obara, Yoshihiro, Sato, Takahiro, Oikawa, Masakazu, Sera, Koichiro.
  • Journal Title: Int.J.Radiat.Oncol.Biol.Phys. 75(2) Pages: 455-462
• [Journal Article] Improvement of radiosensitive Liquid-core microcapsules by Yttrium polymerization (2007)
  • Author(s): S.Harada, S.Ehara, K.Ishii, A.Tanaka, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, T.Sakai, K.Arakawa, M.Saitoh, S.Oikawa, K.Sera.
  • Journal Title: Int.J.PIXE 17 Pages: 33-40
• [Journal Article] Optimizing the radiosensitive liquid core microcapsules for the targeting of chemotherapeutic agents. (2007)
  • Author(s): S.Harada, S.Ehara, K.Ishii, A.Tanaka, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, T.Sakai, K.Arakawa, M.Saitoh, S.Oikawa, K.Sera.
  • Journal Title: Nucl.Inst.Meth.Phys.Res.B. 260(1) Pages: 164-16
• [Journal Article] The target-chemotherapy directed by the radiation. (2005)
  • Author(s): Harada, Satoshi, Ehara, Shigeru, Ishii, Keizoh, Yamazaki, Hiromichi, Matsuyama, Shigeo, Sakai, Takuro, Obara, Yoshihiro, Sato, Takahiro, Oikawa, Masakazu, Sera, Koichiro.
  • Journal Title: Int.J.PIXE. 15(4) Pages: 221-224
• [Journal Article] The target chemotherapy directed by radiation. (2005)
  • Author(s): S.Harada, S.Ehara, K.Ishii, A.Tanaka, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, T.Sakai, K.Arakawa, M.Saitoh, S.Oikawa, K.Sera.
  • Journal Title: Int.J.PIXE 15 Pages: 221-224
• [Presentation] Nanoparticle imaging of the metastatic potential of tumor cells and irradiation-induced release of liquid-core nanocapsules for inhibition of metastasis and O2 radiosensitization (2010)
  • Author(s): S.Harada, S.Ehara., K.Ishii, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, K.Sera.
  • Organizer: 96th Annual Meeting of Radiological Society of North America
  • Place of Presentation: Chicago, USA
  • Year and Date: 20100000
• [Presentation] Inhibition of metastasis and enhancement of antitumor effect usin liquid core nanocapsules via radiotherapy. (2010)
  • Author(s): S.Harada, S.Ehara., K.Ishii, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, K.Sera.
  • Organizer: 52nd Annual meeting of American Society of Therapeutic Radiation Oncology
  • Place of Presentation: San Diego, USA.
  • Year and Date: 20100000
• [Presentation] Radiosensitization by oxygen effect under normal breathing, inhibition of invasion and metastasis, using liquid core microcapsules accumulated through radiation. (2009)
  • Author(s): S.Harada, S.Ehara., K.Ishii, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, K.Sera.
  • Organizer: 95th Annual Meeting of Radiological Society of North America
  • Place of Presentation: Chicago, USA.
  • Year and Date: 20090000
• [Presentation] Inhibition of metastasis and enhancement of antitumor effect usin liquid core nanocapsules via radiotherapy. (2009)
  • Author(s): S.Harada, S.Ehara., K.Ishii, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, K.Sera.
  • Organizer: 51st Annual meeting of American Society of Therapeutic Radiation Oncology
  • Place of Presentation: Chicago USA.
  • Year and Date: 20090000
• [Presentation] Radiosensitization via oxygen effect by using microcapsules with a highly oxygenated liquid core that is released on irradiation (2008)
  • Author(s): S.Harada, S.Ehara., K.Ishii, T.Satoh, S.Matsuyama, H.Yamazaki, T.Kamiya, K.Sera
  • Organizer: 94th Annual Meeting of Radiological Society of North America
  • Place of Presentation: Chicago, USA
  • Year and Date: 20080000