2010 Fiscal Year Final Research Report
Interaction of heavy-ion microbeam and chemotherapeutic agents in vitro
| Project/Area Number |
20591504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
TAKAHASHI Takeo Gunma University, 医学部, 教授 (70241883)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 重粒子線 / バイスタンダー効果 / p53 / 抗癌剤 / DNA修復 |
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| Research Abstract |
It is known that relative biological effectiveness (RBE) of high-LET heavy-ion irradiation is higher than that of low-LET radiation such as X-ray. Monotherapy of charged particles has been applied for several tumors. We investigate the differences between two tumor cell lines with different radiosensitivities in sensitivity to high-LET heavy ion beam and in sensitizing effect and the mechanism of cisplatin and etoposide in combination with heavy-ion beam. Etoposide showed a synergistic effect in combination with carbon beam irradiation in radioresistant NMT-1R cells. However, there was no enhancement effect in radiosensitive NMT-1 cells. On the other hand, cisplatin had no enhancement in both cell lines. There was no differences in the pattern of G2 arrest among Carbon-beam + etoposide, Carbon-beam + cisplatin, and Carbon-beam alone 24 hours after irradiation. The increase in amount of apoptosis and the inhibition of Ku70 protein expression were found in the combination of carbon beam irradiation and etoposide in NMT-1 cells. There were bystander effects after heavy-ion microbeam irradiation.
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[Journal Article] Signal Transduction and Heavy Ion Radiation Therapy : Biological Mechanisms, Biological Quality Assurance, and New Multimodality Approach.2010
Author(s)
Takahashi T, Yoshida Y, Ando K, Kawamura H, Okamoto M, Saitoh J, Ishikawa H, Ebara T, Suzuki Y, Ohno T, Nakano T
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Journal Title
Current Signal Transduction therapy 5
Pages: 237-243
Peer Reviewed
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[Journal Article] Effectiveness of carbon-ion Beams for Apoptosis Induction in Rat Primary Immature Hippocampal Neurons.2010
Author(s)
Kaminuma T, Suzuki Y, Shirai K, Mizui T, Noda SE, Yoshida Y, Funayama T, Takahashi T, Kobayashi Y, Shirao T, Nakano T
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Journal Title
J Radiat Res. 51
Pages: 627-631
Peer Reviewed
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[Journal Article] Usefulness of intraluminal brachytherapy combined with external beam radiation therapy for submucosal esophageal cancer : long-term follow-up results.2010
Author(s)
Ishikawa H, Nonaka T, Sakurai H, Tamaki Y, Kitamoto Y, Ebara T, Shioya M, Noda SE, Shirai K, Suzuki Y, Takahashi T, Nakano T
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Journal Title
Int J Radiat Oncol Biol Phys. 76
Pages: 452-9
Peer Reviewed
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[Journal Article] High-dose-rate intracavity brachytherapy combined with external beam radiotherapy for stage III B adenocarcinoma of the uterine cervix in japan : A multi-institutional study of Japanene society of therapeutic radiorogy and oncology 2006-2007.2010
Author(s)
Niibe Y, Kenjo M, Onishi H, Ogawa Y, Kazumoto T, Ogino I, Tsujino K, Harima Y, Takahashi T, Anbai A, Tsuchiba E, Toita T, Takemoto M, Yamashita H, Hayakawa K
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Journal Title
Jpn J Clini Oncol 8
Pages: 795-799
Peer Reviewed
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[Journal Article] The Benefit of Small Bowel and Pelvic Bone Sparing in Excluding Common Ilic Lymph Node Region from Conventional Radiation Fields in Patients with Uterine Cervical Cancer : A Dosimetric Study.2010
Author(s)
Oike T, Ohno T, Wakatsuki M, Node SE, Saitoh JI, MIzukami T, Yoshimoto Y, Okonogi N, Katoh H, Shibuya K, Suzuki Y, Ishikawa H, Ebara T, Takahashi T, Nakano T
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Journal Title
Journal of Radiation Research 51
Pages: 715-721
Peer Reviewed
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