2010 Fiscal Year Final Research Report
Individualization of Immunosuppressive Therapy Using Pharmacodynamics, Immunology and Molecular Biology
| Project/Area Number |
20591517
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
KUZUYA Takafumi Nagoya University, 医学部附属病院, 副部長 (00444406)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Takaaki 名古屋大学, 大学院・医学系研究科, 教授 (70314010)
HANEDA Masataka 名古屋大学, 大学院・医学系研究科, 講師 (50436995)
KODERA Yasuhiro 名古屋大学, 大学院・医学系研究科, 准教授 (10345879)
IWASAKI Kenta 名古屋大学, 大学院・医学系研究科, 助教 (10508881)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 免疫抑制薬 / 薬力学 / IL-2 / 細胞増殖抑制効果 |
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| Research Abstract |
To optimize immunosuppressive treatment in individual patients, many biomarkers have been proposed, but there has been very little information about association to clinical outcome data. The most effective immunosuppressive strategies in organ transplantation are based on the inhibition of IL-2 signal by calcineurin inhibitiors and we first evaluated the inhibition of IL-2 mRNA production for each patient by using RT-PCR method. Then we examined individual T-cell sensitivity to each immunosuppressive agent (cyclosporine, tacrolimus, mycophenolic acid, predonisolone) using flow cytometry with CFSE labeling. Patients with high sensitivity to cyclosporine tended to experience viral reactivation. Pretransplant pharmacodynamics could provide useful information on optimal and safe immunosuppressive therapy.
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[Journal Article] Stimulation Index for PCNA mRNA in Peripheral Blood as Immune Function Monitoring after Renal Transplantation2009
Author(s)
Misao Niwa, Yuko Miwa, Takafumi Kuzuya, Kenta Iwasaki, Masataka Haneda, Tsuneo Ueki, Akio Katayama, Takahisa Hiramitsu, Norihiko Goto, Takaharu Nagasaka, Yoshihiko Watarai, Kazuharu Uchida, Akimasa Nakao, Takaaki Kobayashi
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Journal Title
Transplantation 87
Pages: 1411-1414
Peer Reviewed
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[Journal Article] Pretransplant Pharmacodynamic Analysis of Immunosuppressive Agents using CFSE-based T Cell Proliferation Assay2009
Author(s)
Yoko Kurata, Marina Kato, Takafumi Kuzuya, Yuko Miwa, Kenta Iwasaki, Masataka Haneda, Katsuo Amioka, Yoshihiko Watarai, Kazuharu Uchida, Akimasa Nakao, Takaaki Kobayashi
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Journal Title
Clinical Pharmacology & Therapeutics 86
Pages: 285-289
Peer Reviewed
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[Journal Article] 免疫抑制薬の分子論的解析と臨床応用可能なPDモニタリングの開発2008
Author(s)
倉田洋子, 網岡克雄, 加藤真梨奈, 野田幸裕, 葛谷孝文, 羽根田正隆, 長坂隆治, 打田和治, 中尾昭公, 岩崎研太, 小林孝彰
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