2010 Fiscal Year Final Research Report
A novel strategy to minimize mTOR-induced autophagy to pancreatic islet beta cells
| Project/Area Number |
20591524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Osaka University |
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Principal Investigator |
ITO Toshinori Osaka University, 医学系研究科, 寄附講座教授 (20231152)
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| Co-Investigator(Kenkyū-buntansha) |
TANEMURA Masahiro 大阪大学, 医学系研究科, 助教 (30379250)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 膵島移植 / 1型糖尿病 / オートファジー / 長期成績 / mTOR阻害剤 / 3-MA |
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| Research Abstract |
Mammalian target of rapamycin (mTOR) inhibitor has extensively used in clinical islet transplantation to prevent the graft rejection. Recently, mTOR inhibitor has also reported to induce autophagy to various kinds of cell lines. Autophagy is an essential, homeostatic process by which cells break down their own components. However, its role on pancreatic islets has not been thoroughly understood. In this study we demonstrated that both in vitro and in vivo, rapamycin treatment induced autophagy to islets. Furthermore, addition of autophagy inhibitor resulted in better graft outcome. These results suggest that rapamycin with autophagy inhibitor would be an ideal combination to prevent graft rejection without autophagy induction.
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Research Products
(24 results)
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[Journal Article] Combined Transplantation of Pancreatic Islets and Adipose Tissue-Derived Stem Cells Enhances the Survival and Insulin Function of Islet Grafts in Diabetic Mice.2010
Author(s)
Ohmura Y, Tanemura M, Kawaguchi N, Machida T, Tanida T, Deguchi T, Wada H, Kobayashi S, Marubashi S, Eguchi H, Takeda Y, Matsuura N, Ito T, Nagano H, Doki Y, Mori M.
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Journal Title
Transplantation. 90(12)
Pages: 1366-73
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[Journal Article] Increased immunogenicity of tumor-associated antigen, MUC1, engineered to express α-gal epitopes : A novel approach to immunotherapy in pancreatic cancer.2010
Author(s)
Deguchi T, Tanemura M, Miyoshi E, Nagano H, Machida T, Ohmura Y, Kobayashi S, Marubashi S, Eguchi H, Takeda Y, Ito T, Mori M, Doki Y, Sawa Y.
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Journal Title
Cancer Research. 70(13)
Pages: 5259-69
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[Presentation] MUC1, remodeled to express α-gal epitopes immune-based therapy can elicit both significant prolonged survival and effective antibody production against multiple tumor-associated antigens.2010
Author(s)
Tanemura M, Deguchi T, Miyoshi E, Nagano H, Ohmura Y, Machida T, Wada H, Kobayashi S, Marubash S, Eguchi H, Takeda Y, Ito T, Mori M, Doki Y
Organizer
AACR 101nd Annual Meeting
Place of Presentation
Washington, USA
Year and Date
20100417-20100420
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[Presentation] Hybrid islet transplantation with marginal islet mass and adipose tissue-derived stem cells can elicit long-term islet graft survival and support normoglycemia in diabetic mice.2010
Author(s)
Y.Ohmura, M.Tanemura, T.Machida, T.Deguchi, H.Wada, S.Kobayashi, S.Marubashi, H.Eguchi, Y.Takeda, N.Kawaguchi, N.Matsuura, T.Ito, H.Nagano, Y.Doki, M.Mori
Organizer
XXIII International Congress of The Transplantation Society
Place of Presentation
Vancouver, CANADA
Year and Date
2010-08-16
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[Presentation] Rapamycin Induces Autophagy in Islets and Impairs Islet Function.2009
Author(s)
Tanemura M., Ito T., Machida T., Deguchi T., Kobayashi S., Marubashi S., Takeda Y., Nagano H., Sawa Y., Mori M., Doki Y.
Organizer
American Transplantation Congress.
Place of Presentation
Boston
Year and Date
20090530-20090602
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