2010 Fiscal Year Final Research Report
Analysis of Polycomb group gene role in pancreatic cancer development from pancreatic stem cell
| Project/Area Number |
20591532
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
OSHIMA Yuji Yokohama City University, 医学研究科, 客員研究員 (10468209)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Yasuharu 横浜市立大学, 医学部, 助教 (60375235)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 膵臓 / 幹細胞 / フローサイトメーター / ヒストン修飾 / ポリコーム群タンパク質 |
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| Research Abstract |
Dysregulation in the pathway that control normal stem-cell self-renewal is considered a key early event in carcinogenesis. To reveal the involvement of Polycomb-group (PcG) genes in early event in pancreatic cancer development, we analyzed the function of the PcG gene Bmi1 in murine pancreatic stem/progenitor cells. As a result, overexpressed Bmi1 did not enhance the proliferation capability and differentiation capability of pancreatic stem/progenitor cells. We also examined activation of pancreatic stem/progenitor cells in pancreatic tumor formation in a pdx1-cre mediated Kras G12D expression model mouse. Histological analysis revealed that Pancreatic Intraepithelial Neoplasia (PanIN) was observed in intra/ interlobular pancreatic ducts of pancreas with high frequency. These results will enable us to better understand the process of pancreatic cancer development from pancreatic stem/progenitor cells.
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