2010 Fiscal Year Final Research Report
Development of novel cancer diagnosis and treatment targeting splicing variants of c-myc transcriptional suppressor FIR
| Project/Area Number |
20591543
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
MATSUSHITA Kazuyuki Chiba University, 大学院・医学研究院, 講師 (90344994)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 癌遺伝子治療 / c-myc遺伝子 / 癌診断 / 癌治療 / スプライシング阻害薬 / SF3b155 / 転写制御因子 / アポトーシス |
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| Research Abstract |
Far UpStream Element (FUSE)-binding protein-interacting repressor (FIR), a c-myc transcriptional suppressor, is alternatively spliced and futilely produced in colorectal cancer tissues that abundantly express c-Myc. Human malignant pleural mesothelioma (HMPM) is highly resistant to conventional therapy, and novel therapies are thus required. We previously reported that overexpression of FIR induces apoptosis via c-Myc suppression, and is thus a suitable cancer therapy. In the current preclinical trial, a fusion gene deleted non-transmissible Sendai virus vector encoding FIR (SeV/ΔF/FIR) was prepared and its cytotoxic activity against an orthotopic xenograft model of HMPM, in combination with cisplatin, was assessed. SeV/ΔF/FIR significantly reduced cell viability in 3 HMPM cell lines but was less effective in non-tumor immortalized mesothelial cells. SeV/ΔF/FIR cytotoxicity was partly due to apoptosis induction via c-Myc supression.
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Research Products
(26 results)
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[Journal Article] Synergistic effect of non-transmissible Sendai virus vector encoding the c-myc suppressor FUSE-binding protein-interacting repressor plus cisplatin in treatment of malignant pleural mesothelioma.2011
Author(s)
Kitamura A, Matsushita K, Takiguchi Y, Shimada Hideaki, Tomonaga T, Matsubara H. Inou, M, Mamoru H, Sato Y, Levens D, Tatsumi K, Nomura F.
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Journal Title
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[Journal Article] Decreases in the Serum VLDL-TG/Non-VLDL-TG Ratio from Early Stages of Chronic Hepatitis C : Alterations in TG-Rich Lipoprotein Levels.2011
Author(s)
Nishimura M, Yamamoto H, Yoshida T, Seimiya M, Sawabe Y, Matsushita K, Umemura H, Sogawa K, Takizawa H, Yokosuka O, Nomura F.
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Journal Title
PLoS ONE Research Article published 25
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[Journal Article] CHAP31 Induces Apoptosis Only via the Intrinsic Pathway in Human Esophageal Cancer Cells.2010
Author(s)
Murakami K, Matsubara H, Hoshino I, Akutsu Y, Miyazawa Y, Matsushita K, Sakata H, Nishimori T, Usui A, Kano M, Nishino N, Yoshida M.
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Journal Title
Oncology. 78(1)
Pages: 62-74
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[Journal Article] Reduction of novel circulating long-chain fatty acids in colorectal cancer patients is independent of tumor burden and correlates with age.2010
Author(s)
Ritchie SA, Heath D, Yamazaki Y, Grimmalt B, Kavianpour A, Krenitsky K, Elshoni H, Takemasa I, Miyake M, Sekimoto M, Monden M, Tomonaga T, Matsubara H, Sogawa K, Matsushita K, Nomura F, Goodenowe DB.
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Journal Title
BMC Gastroenterology 10
Pages: 140
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[Journal Article] YKL-40 identified by proteomic analysis as a biomarker of sepsis.2009
Author(s)
Hattori N, Oda S, Sadahiro T, Nakamura M, Abe R, Shinozaki K, Nomura F, Tomonaga T, Matsushita K, Kodera Y, Sogawa K, Satoh M, Hirasawa H.
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Journal Title
Shock. 32(4)
Pages: 393-400
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[Remarks] ホームページ等
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