Clinical application and prognostication in human breast cancer using tissue quantitative fluorescence in situ hybridization.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591555
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Nihon University
• Principal Investigator: KAMMORI Makoto Nihon University, 医学部, 非常勤講師 (50292868)
• Co-Investigator(Kenkyū-buntansha): KAMOTO Takahiro 東京女子医科大学, 医学部, 教授 (70194397)
• Co-Investigator(Renkei-kenkyūsha): OBAYASHI Makio 東京女子医科大学, 医学部, 教授 (80060086)
• Project Period (FY): 2008 – 2010
• Keywords: 乳癌 / 遺伝子・染色体 / FISH / テロメア / 予後診断

Research Abstract

We analyzed the telomere length by tissue Q-FISH. Almost breast cancer cells had shortened telomere in comparison with the normal cells. In specific four cases that showed the extremely short telomere length, the tumor size was significantly higher than other cases.

Research Products

• [Journal Article] Chromosomal instability and telomere lengths of each chromosomal arm measured by Q-FISH in human fibroblast strains prior to replicative senescence. (2010)
  • Author(s): Takubo K, Aida J, Izumiyama N, Ishikawa N, Fujiwara M, Poon S.S.S., Kondo H, Kammori M, Matsuura M, Sawabe M, Arai T, Baird D.M., Nakamura K
  • Journal Title: Mechanisms of Ageing and Development 131 Pages: 614-624
  • Peer Reviewed
• [Journal Article] Luminal and cancer cells in the breast show more rapid telomere shortening than myoepithelial cells and fibroblasts. (2008)
  • Author(s): Kurabayashi R, Takubo K, Aida J, Honma N, Poon SSS, Kammori M, Izumiyama-Shimomura N, Nakamura K, Tsuji1 E, Matsuura M, Ogawa T, Kaminishi1 M.
  • Journal Title: Human Pathology 39 Pages: 1647-1655
  • Peer Reviewed
• [Journal Article] Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium. (2008)
  • Author(s): Aida J, Izumiyama-Shimomura N, Nakamura K, Ishikawa N, Poon SSS, Kammori M, Sawabe M, Arai T, Fujiwara F, Kishimoto K, Takubo K
  • Journal Title: Exp Gerontol. 43(9) Pages: 833-9
  • Peer Reviewed
• [Journal Article] Prognostic utility of fluorescence in situ hybridization (FISH) for determination of HER2 gene amplification in breast cancer. (2008)
  • Author(s): Kammori M, Kurabayashi R, Kashio M, Sakamoto A, Yoshimoto M, Amano S, Kaminishi M, Tetsu Yamada, Takubo K
  • Journal Title: Oncology Reports 19 Pages: 651-656
  • Peer Reviewed
• [Presentation] テロメレース活性とhTERT発言による副甲状腺癌補助診断への応用 (2010)
  • Author(s): 神森眞
  • Organizer: 第22回日本内分泌外科学会
  • Place of Presentation: 大阪
  • Year and Date: 20100611-20100612
• [Presentation] 組織Q-FISH法によるテロメア長解析とテロメレース活性を用いた甲状腺濾胞癌の補助診断 (2010)
  • Author(s): 神森眞
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20100408-20100410
• [Presentation] Demonstration of telomerase activity and human telomerase reverse transcriptase(hTERT) in human parathyroid tumors (2010)
  • Author(s): Kammori M
  • Organizer: The 21th APEC
  • Place of Presentation: Taiwan
  • Year and Date: 2010-01-09
• [Presentation] Demonstration of telomere measurement in human papillary carcinoma of the thyroid by tissue quantitative fluorescence in situ hybridization (Q-FISH). (2009)
  • Author(s): Kammori M, Takubo K, Yamada T
  • Organizer: The 20th APEC
  • Place of Presentation: Guam
  • Year and Date: 20090111-20090112
• [Presentation] h-TERT発現による甲状腺濾胞癌の診断-基礎から臨床応用への展望 (2008)
  • Author(s): 神森眞, 小川利久, 小野瀬裕之, 山田恵美子, 清水一雄, 二村浩史, 瀬戸泰之, 山田哲
  • Organizer: 第70回日本臨床外科学会総会. (パネルディスカッション7. 甲状腺濾胞癌に対する最新の診断法と治療)
  • Place of Presentation: 東京
  • Year and Date: 20081127-20081129
• [Presentation] 甲状腺乳頭癌と甲状腺正常組織の組織Q-FISH法を用いたテロメア長解析 (2008)
  • Author(s): 神森眞、柏尾光彦、倉林理恵、坂本明子, 天野定雄, 田久保海誉
  • Organizer: 第108回日本外科学会定期学術集会(SF)
  • Place of Presentation: 長崎
  • Year and Date: 20080515-20080517