Effect of hyperthermia on inhibition of cancer stem cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591593
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nara Medical University
• Principal Investigator: OOMORI Hitoshi Nara Medical University, 医学部, 博士研究員 (80213875)
• Co-Investigator(Kenkyū-buntansha): KUNIYASU Hiroki 奈良県立医科大学, 医学部, 教授 (00253055)
• Co-Investigator(Renkei-kenkyūsha): SASAHIRA Tomonori 奈良県立医科大学, 医学部, 助教 (90405374) ; BHAWAL Ujjal 奈良県立医科大学, 医学部, 博士研究員 (50433339)
• Project Period (FY): 2008 – 2010
• Keywords: 小腸大腸肛門外科学

Research Abstract

For examining the factors which possess anti-cancer stem or anti-cancer stem niche effects, we focused on heat therapy, fatty acids, glycoprotein, and hypoxia. Heat therapy reduces stemness of cancer cells. In contrast, linoleic acid induces quiescent cancer stem cells to produce cancer dormancy ; and elaidic acid (trans form of oleic acid) increases proliferating cancer stem cells to enhance overt metastasis. These properties of heat therapy and fatty acids might provide a new therapeutic target.

Research Products

• [Journal Article] Non-histone nuclear factor HMGB1 as a target in colorectal cancer. (2011)
  • Author(s): Ohmori H, Luo Y, Kuniyasu H
  • Journal Title: Expert Opin Ther Tar 15(2) Pages: 183-193
• [Journal Article] Downregulation of runt-related transcription factor 3 (RUNX3) associated with poor prognosis of adenoid cystic and mucoepidermoid carcinomas of the salivary gland. (2011)
  • Author(s): Sasahira T, Kurihara M, Yamamoto K, Bhawal UK, Kirita T, Kuniyasu H
  • Journal Title: Cancer Sci 102(2) Pages: 492-497
• [Journal Article] In vitro enhanced differentiation of neural networks in ES gut-like organ from mouse ES cells by a 5-HT4-receptor activation. (2011)
  • Author(s): Takaki M, Misawa H, Matsuyoshi, H, Kawahara I, Goto K, Zhang GX, Obata K, Kuniyasu H
  • Journal Title: Biochem Biophys Res Commun 406(4) Pages: 529-533
• [Journal Article] HMGB1 attenuates anti-metastatic defense of the lymph nodes in colorectal cancer. (2010)
  • Author(s): Moriwaka Y, Luo Y, Ohmori H, Fujii K, Tatsumoto N Sasahira T, Kuniyasu H
  • Journal Title: Pathobiology 77(1) Pages: 17-23
• [Journal Article] HMGB1 attenuates anti-metastatic defense of the liver in colorectal cancer. (2010)
  • Author(s): Luo Y, Ohmori H, Fujii K, Moriwaka Y, Sasahira T, Kurihara M, Tatsumoto N, Sasaki T, Yamashita Y, Kuniyasu H
  • Journal Title: Eur J Cancer 46(4) Pages: 791-799
• [Journal Article] A 5-HT4-receptor activation-induced neural plasticity enhances in vivo reconstructs of enteric nerve circuit insult. (2010)
  • Author(s): Matsuyoshi H, Kuniyasu H, Okumura M, Misawa H, Katsui R, Zhang GX, Obata K, Takaki M
  • Journal Title: Neurogastroenterol Motil 22(7) Pages: 806-814
• [Journal Article] Dietary linoleic acid and glucose enhances azoxymethane-induced colon cancer and the metastasis through the expression of high mobility group box 1. (2010)
  • Author(s): Ohmori H, Luo Y, Fujii K, Sasahira T, Shimomoto T, Denda A, Kuniyasu H
  • Journal Title: Pathobiology 77(4) Pages: 210-217
• [Journal Article] MIA dependent angiogenesis and lymphangiogenesis are closely associated with progression, nodal metastasis and poor prognosis in tongue squamous cell carcinoma. (2010)
  • Author(s): Sasahira T, Kirita T, Kurihara M, Yamamoto K, Bhawal UK, Bosserhoff AK, Kuniyasu H
  • Journal Title: Eur J Cancer 46 Pages: 2285-2294
• [Journal Article] Determinants of the epithelial-muscle axis on embryonic stem cell-derived gut-like structures. (2010)
  • Author(s): Luo Y, Takaki M, Misawa H, Matsuyoshi H, Sasahira T, Chihara Y, Fujii K, Ohmori H, Kuniyasu H
  • Journal Title: Pathobiology 77(5) Pages: 253-259
• [Journal Article] A case of gastric cancer with non-islet cell tumor hypoglycemia detected by insulin-like growth factor II. (2010)
  • Author(s): Maruyama H, Tatsumi M, Kitayama H, Enomoto Y, Kuniyasu H, Uematsu K, Fukuda I, Kameya T, Konishi Y.
  • Journal Title: Pathol Int 60(8) Pages: 595-597
• [Journal Article] CD10 enhances metastasis of colorectal cancer by abrogating the antitumoral effect of methionine-enkephalin in the liver. (2009)
  • Author(s): Kuniyasu H, Luo Y, Fujii K, Sasahira T, Moriwaka Y, Tatsumoto N, Sasaki T, Yamashita Y, Ohmori H.
  • Journal Title: Gut 59(3)(On line publication) Pages: 348-56
• [Journal Article] The plasticity of the defecation reflex pathway in the enteric nervous system of guinea pigs. (2009)
  • Author(s): Katsui R, Kuniyasu H, Matsuyoshi H, Fujii H, Nakajima Y, Takaki M.
  • Journal Title: J Smooth Muscle Res 45(1) Pages: 1-13
• [Journal Article] Reg IV enhances peritoneal metastasis in gastric carcinomas. (2009)
  • Author(s): Kuniyasu H: Oue N, Sasahira T, Moriwaka Y, Shimomoto T, Fujii K, Ohmori H, Yasui W
  • Journal Title: Cell Prolif 42(1) Pages: 110-121
• [Journal Article] Co-treatment with deoxycholic acid and azoxymethane accelerates the secretion of HMGB1 in IEC6 intestinal epithelial cells (2009)
  • Author(s): Fujii K, Luo Y, Sasahira T, Denda A, Ohmori H, Kuniyasu H
  • Journal Title: Cell Prolif 42(5) Pages: 701-709
• [Journal Article] Methionine- enkephalin secreted by human colorectal cancer cells suppresses T lymphocytes. (2009)
  • Author(s): Ohmori H, Fujii K, Sasahira T, Luo Y, Isobe M, Tatsumoto N, Kuniyasu H
  • Journal Title: Cancer Sci 100(3) Pages: 497-502
• [Journal Article] Suppression of dendritic cells by HMGB1 is associated with lymph node metastasis of human colon cancer. (2009)
  • Author(s): Kusume A, Sasahira T, Luo Y, Isobe M, Nakagawa N, Tatsumoto N, Fujii K, Ohmori H, Kuniyasu H
  • Journal Title: Pathobiology 76(4) Pages: 155-162
• [Journal Article] Antisense phosphorothioate oligodeoxynucleic acid for CD10 suppresses liver metastasis of colorectal cancer. (2009)
  • Author(s): Luo Y, Fujii K, Ohmori H, Sasahira T, Moriwaka Y, Isobe M, Kuniyasu H
  • Journal Title: Pathobiology 76(5) Pages: 267-273
• [Journal Article] The roles of dietary PPARY ligands for metastasis in colorectal cancer. (2008)
  • Author(s): Kuniyasu H
  • Journal Title: In PPARs : A Double-Edged Sword in Cancer Therapy? (ed) Panigrahy D. PPAR Res Pages: 529720
• [Journal Article] Loss of 14-3-3 sigma expression and human papillomavirus 16 E6 presence in oral squamous cell carcinoma. (2008)
  • Author(s): Bhawal UK, Sugiyama M, Nomura Y, Sawajiri M, Otsubo A, Tsukinoki K, Ikeda M, Kuniyasu H
  • Journal Title: Arch Otolaryngol Head Neck Surg 134(10) Pages: 1055-1059
• [Journal Article] Recurrence of keratocystic odontogenic tumor : clinico- pathological features and immunohistochemical study of the hedgehog signaling pathway. (2008)
  • Author(s): Yagyuu T, Kirita T, Sasahira T, Moriwaka Y, Yamamoto K, Kuniyasu H
  • Journal Title: Pathobiology 75(3) Pages: 171-176
• [Journal Article] Linoleic acid-induced growth suppression induces quiescent cancer cell nests in nude mice. (2008)
  • Author(s): Ohmori H, Sasahira T, Fujii K, Luo Y, Shimomoto T, Kuniyasu H
  • Journal Title: Pathobiology 75(4) Pages: 226-232
• [Journal Article] Protection of telomeres protein levels are associated with telomere length in gastric cancer. (2008)
  • Author(s): Fujii K, Sasahira T, Moriwaka Y, Oue N, Yasui W, Kuniyasu H
  • Journal Title: Int J Mol Med 21(5) Pages: 599-604
• [Journal Article] Plastticity of enteric nerve pathways for the recto-rectal and recto-internal sphincter reflexs in guinea pigs. (2008)
  • Author(s): Katsui R, Kojima Y, Kuniyasu H, Shimizu J, Koyama F, Fujii H, Nakajima Y, Takaki M
  • Journal Title: Am J Physiol Gastrointest Liver Physiol 294(4) Pages: G1084-G1093
• [Journal Article] Serous borderline tumor of the paratestis. (2008)
  • Author(s): Maruyama H, Ohyama N, Hosokawa Y, Momose H, Yamada K, Tsutsumi M, Kuniyasu H, Enomoto Y, Uematsu K, Konishi Y
  • Journal Title: Pathol Int 58(5) Pages: 311-316
• [Journal Article] Reg IV is an independent prognostic factor for relapse in patients with clinically localized prostate cancer. (2008)
  • Author(s): Ohara S, Oue N, Matsubara A, Mita K. Hasegawa Y, Hayashi T, Usui T, Amatya V, Takeshima Y, Kuniyasu H, Yasui W
  • Journal Title: Cancer Sci 99(8) Pages: 1570-1577
• [Journal Article] High mobility group box-1-inducible melanoma inhibitory activity is associated with nodal metastasis and lymphangiogenesis in oral squamous cell carcinoma. (2008)
  • Author(s): Sasahira T, Kirita T, Oue N, Bhawal UK, Yamamoto K, Fujii K, Ohmori H, Luo Y, Yasui W, Bosserhoff AK, Kuniyasu H
  • Journal Title: Cancer Sci 99(9) Pages: 1806-1812
• [Journal Article] Reg IV expression is associated with cell growth and prognosis of adenoid cystic carcinoma in the salivary gland. (2008)
  • Author(s): Sasahira T, Oue N, Kirita T, Luo Y, Bhawal UK, Yasui W, Kuniyasu H
  • Journal Title: Histopathol 53(6) Pages: 667-675
• [Journal Article] Evaluation of metastatic potential of prostate cancer.
  • Author(s): Chihara Y, Fujimoto K, Hirao Y, Kuniyasu H
  • Journal Title: Oncol Rev in press
• [Journal Article] The roles of HMGB1 related angiogenesis and lymphangiogenesis in oral cancer.
  • Author(s): Sasahira T, Yamamoto K, Kurihara M, Bhawal UK, Chihara Y, Kirita T, Kuniyasu H
  • Journal Title: Oncol Rev in press
• [Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates CyclinD1.
  • Author(s): Bhawal UK, Sato F, Arakawa Y, Fujimoto K, Kawamoto T, Tanimoto K, Ito Y, Sasahira T, Sakurai T, Kobayashi M, Kashima I, Kajima H, Kuniyasu H, Abiko Y, Kato Y, Sato S
  • Journal Title: J Pathol in press
• [Journal Article] Involvement of HMGB1 and RAGE in IL-1β-induced gingival inflammation.
  • Author(s): ItoY, Bhawal UK, Sasahira T, Toyama T, Sato T, Matsuda D, Kobayashi M, Sugiyama M, Hamada N, Arakawa H, Kuniyasu H
  • Journal Title: Arch Oral Biol in press
• [Journal Article] High matrix metalloproteinase-to-E-cadherin ratio measured by bicolor fluorescent in situ hybridization is associated with lymphangiogenesis and lymph node metastasis in prostate cancer.
  • Author(s): Luo Y, Ohmori H, Fujii K, Chihara Y, Maruyama S, Kuniyasu H
  • Journal Title: Urol Oncol-Semin Ori in press
• [Journal Article] Anti-angiotensin and hypoglycemic treatments suppress liver metastasis of colon cancer cells.
  • Author(s): Luo Y, Ohmori H, Shimomoto T, Fujii K, Kuniyasu H
  • Journal Title: Pathobiology in press
• [Presentation] HMGB1 affects plasma amino acid profiles in colon carcinogenesis. (2011)
  • Author(s): Luo Y, Yoneda J, Ohmori H, Shimbo K, Eto S, Fujii K, Sasahira T, Chihara Y, Kuniyasu H
  • Organizer: 102nd Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Florida
  • Year and Date: 20110402-20110406
• [Presentation] Expression of MAS1 in breast cancer. (2010)
  • Author(s): Luo Y, Chihara Y, Fujii K, Moriwaka Y, Ohmori H, Kuniyasu H
  • Organizer: 20th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2010-10-31
• [Presentation] MIA induces angiogenesis and lymphangiogenesis. (2010)
  • Author(s): 笹平智則, 國安弘基
  • Organizer: Japan-Korea Urology Research Meeting 2010
  • Place of Presentation: Kashihara
  • Year and Date: 2010-05-22
• [Presentation] Diagnostic indicator of urological cancer based on quantitative DNA methylation analysis. (2010)
  • Author(s): Chihara Y, Kuniyasu H
  • Organizer: Japan-Korea Urology Research Meeting 2010
  • Place of Presentation: Kashihara
  • Year and Date: 2010-05-22
• [Presentation] 脂肪酸の大腸癌転移に及ぼす影響,ミニシンポジウム(5)-3消化器癌の分子基盤:浸潤・転移-3 (2009)
  • Author(s): 羅奕、藤井澄、梶原義典、佐藤真吾、國安弘基
  • Organizer: 第20回日本消化器癌発生学会
  • Place of Presentation: 広島
  • Year and Date: 20091126-20091127
• [Presentation] 脂肪酸の大腸癌転移に及ぼす影響 (2009)
  • Author(s): 國安弘基、羅奕、佐藤真吾、梶原義典、笹平智則、傳田阿由美、大森斉
  • Organizer: 第24回発癌病理研究会
  • Place of Presentation: 和倉
  • Year and Date: 20090825-20090827
• [Presentation] Activation of angiotensin system enhances liver metastasis of human colorectal cancer. (2009)
  • Author(s): Kuwai T, Kitadai Y, Luo Y, Sasahira T, Masaki K, Kohno H, Isobe M, Ohmori H, Kuniyasu H
  • Organizer: 100th Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Denver
  • Year and Date: 20090418-20090422
• [Presentation] Cancer cell necrosis induces tumor growth and metastasis by releasing HMGB1. (2009)
  • Author(s): Kitadai Y, Luo Y, Ohmori H, Fujii K, Sasahira T, Chihara Y, Kuniyasu H
  • Organizer: 101th Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Washington DC
  • Year and Date: 20090417-20090421
• [Presentation] Methionine-enkephalin inhibits liver metastasis of colorectal cancers. (2009)
  • Author(s): Luo Y, Moriwaka Y, Fujii K, Ohmori H, Kuniyasu H
  • Organizer: 19th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2009-10-01
• [Presentation] Angiotensin II expression is associated with liver metastasis of colorectal cancer. (2009)
  • Author(s): Luo Y, Isobe M, Fujii K, Ohmori H, Kuniyasu H
  • Organizer: 19th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2009-10-01
• [Presentation] Receptor for advanced glycation end products (RAGE) is associated with prognosis and angiogenesis in human oral squamous cell carcinoma (OSCC). (2008)
  • Author(s): Sasahiar T, Kuniyasu H, Luo Y, Bhawal UK, Yamamoto K, Kirita T
  • Organizer: 12th International Congress on Oral Cancer
  • Place of Presentation: Shanghai
  • Year and Date: 20080522-20080525
• [Presentation] CD10 reduces inhibitory effect of Met-enkephalin in colon cancer. (2008)
  • Author(s): Moriwaka Y, Kuwai T, Sasahira T, Ohmori H, Kitadai Y, Kuniyasu H
  • Organizer: 99th Annual Meeting of American Association for Cancer Research
  • Place of Presentation: San Diego
  • Year and Date: 20080412-20080416
• [Presentation] Activation of angiotensin system enhances liver metastasis of human colorectal cancer. (2008)
  • Author(s): Ohya M, Iwatsubo S, Moriwaka Y, Luo Y, Denda A, Ohmori H, Kuniyasu H
  • Organizer: 18th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2008-11-09
• [Presentation] CD10 abrogates immune disturbance by beta-casomorphin in intestinal mucosa. (2008)
  • Author(s): Kaya D, Fukuoka H, Luo Y, Shimomoto T, Sasahira T, Ikeda M, Fujii K, Ohmori H, Kuniyasu H
  • Organizer: 8th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2008-11-09
• [Presentation] A trans fatty acid, eridic acid enhanses metastasis of colorectal cancer. (2008)
  • Author(s): Toyama Y, Nishii R, Sasahira T, Luo Y, Fujii K, Ohmori H, Kuniyasu H
  • Organizer: 18th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2008-11-09
• [Presentation] CD10 enkephalinase enhances liver metastasis by suppression of Kupffer cell-derived Methionine-enkephalin in human colorectal cancer. (2008)
  • Author(s): Luo Y, Shimomoto T, Tatsumoto N, Fujii K, Kuniyasu H
  • Organizer: 18th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2008-11-09
• [Presentation] Suppression of dendritic cells by HMGB1 is associated with lymph node metastasis of human colon cancer. (2008)
  • Author(s): Luo Y, Sasahira T, Isobe M, Nakagawa N, Tatsumoto N, Ohmori H, Kuniyasu H
  • Organizer: 18th International Symposium of Hiroshima Cancer Seminar
  • Place of Presentation: Hiroshima
  • Year and Date: 2008-11-09
• [Book] HMGB1 induces drug resistance in cancer cells. In : Current research in cancer 5, 2011, eds : Sunitha M. pp, B5, Kerala, India (2011)
  • Author(s): Kuniyasu H
  • Publisher: Research Media
• [Book] Recent advances in metastasis of gastrointestinal cancers. B5 Kerala, India (2010)
  • Author(s): Kuniyasu H, Kitadai Y
  • Total Pages: 214
  • Publisher: Transworld Research Network
• [Book] Hyperplastic change of the mucosa adjacent to colorectal carcinomas: a newly recognized cancer-host relationship. In : Recent advances in metastasis of gastrointestinal cancers, eds : Kuniyasu H, Kitadai Y. A5, India (2010)
  • Author(s): Kuniyasu H
  • Total Pages: 65-85
  • Publisher: Transworld Research Network
• [Book] Angiogenesis and lymphangiogenesis in oral cancer. In : Recent advances in metastasis of gastrointestinal cancers, eds : Kuniyasu H, Kitadai Y., A5, India (2010)
  • Author(s): Sasahira T, Kuniyasu H
  • Total Pages: 22-40
  • Publisher: Transworld Research Network
• [Book] Diverse roles of linoleic acid on development and progression of colorectal cancer. In : Recent advances in metastasis of gastrointestinal cancers, eds : Kuniyasu H, Kitadai Y., A5, India (2010)
  • Author(s): Luo Y, Kuniyasu H
  • Total Pages: 87-102
  • Publisher: Transworld Research Network
• [Book] Histological characteristics of the colorectal cancer metastasized to the liver. In : Recent advances in metastasis of gastrointestinal cancers, eds : Kuniyasu H, Kitadai Y., A5, India (2010)
  • Author(s): Ohmori H, Tatsumoto N, Kuniyasu H
  • Total Pages: 161-173
  • Publisher: Transworld Research Network
• [Book] Peritoneal dissemination of gastric cancer. In : Recent advances in metastasis of gastrointestinal cancers, eds : Kuniyasu H, Kitadai Y., A5, Kerala, India (2010)
  • Author(s): Fujii K, Kuniyasu H
  • Total Pages: 115-130
  • Publisher: Transworld Research Network
• [Book] RAGE, AGE, and HMGB1. dramatypes of Western diet in colon cancer development. In : Current research in cancer 4, 2010, eds : Sunitha M., Kerala, India (2010)
  • Author(s): Kuniyasu H
  • Publisher: Research Media(In press)
• [Book] Application of in situ hybridization technique to pathology researches. In : Research advances in histopathology 1, eds : Sunitha M., B5, Kerala, India (2010)
  • Author(s): Kuniyasu H
  • Total Pages: 1-9
  • Publisher: Research Media
• [Book] Linoleic acid. In : Encyclopedia of cancer, 2nd edition, (ed) Schwab M. Berlin (2009)
  • Author(s): Kuniyasu H
  • Total Pages: 3235
  • Publisher: Springer-Verlag
• [Book] CD10-a significant determinant of liver metastasis in colon cancer. In : Current research in cancer 3, 2009, eds : Sunitha M., B5, Kerala, India (2009)
  • Author(s): Kuniyasu H
  • Total Pages: 65-73
  • Publisher: Research Media
• [Book] Application of in situ hybridization technique to pathology researches. In : Current Research in Pathology, eds : Sunitha M. B5, Kerala, India (2009)
  • Author(s): Kuniyasu H
  • Total Pages: 1-12
  • Publisher: Research Media
• [Book] New angiogenic factors in oral cancer. In : Current Research in Cancer 2008, (ed) Sunitha M. Kerala, India (2008)
  • Author(s): Kuniyasu H
  • Total Pages: 1-11
  • Publisher: Research Media
• [Remarks] ホームページ等
  • URL: http://www.naramed-u.ac.jp/~molepath/