2010 Fiscal Year Final Research Report
Development of hybrit mouse with humanized liver using superfreezing preserved human small hepatocytes.
| Project/Area Number |
20591615
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MIZUGUCHI Toru Sapporo Medical University, 医学部, 准教授 (30347174)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Koichi 札幌医科大学, 医学部, 教授 (50136959)
MITAKA Toshihiro 札幌医科大学, 医学部, 教授 (50231618)
NAGAYAMA Minoru 札幌医科大学, 医学部, 助教 (40398326)
MEGURO Makoto 札幌医科大学, 医学部, 助教 (50448601)
SHIBATA Toshihito 札幌医科大学, 医学部, 研究員 (80404622)
KAJI Shinsuke 札幌医科大学, 医学部, 研究員 (90404623)
KAWAMOTO Masaki 札幌医科大学, 医学部, 助教 (70404605)
NAKAMURA Yukio 札幌医科大学, 医学部, 研究員 (50516648)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 自己組織化 / 生体機能利用 / 移植・再生医療 / 応用動物 / 再生医学 |
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| Research Abstract |
It is very important to perform surgical operation to obtain cell donation for cell transplantation therapy. Conventional hepatectomy has been considered to be difficult to decrease high mobidity with mortlity. A saline-linked electiric cautery was very effective to achieve vessel sealing and decrease bleeding. In addition, recovery of postoperative Apo-B in this group was much faster than conventional hepatectomy (Hepatogastroenterology 2008 ; 55 : 2188-2192.). Gene transduction of Pdx-1 could transform small hepatocyte into insulin, glucagon, and somatostatine producing cells. Furthermore, small hepatocytes tend to be trans-differentiated into beta cells and mature hepatocytes tend to be trans-differentiated into alpha cells after transduction of Pdx-1 gene (J Hepatobiliary Pancreat Surg 2008 ; 15 : 403-409.). On the other hand, urinary trypsin inhibitor (UTI) is one of the proteins which the liver could produce by itself exclusively. The liver regeneration has been impaired in UTI deficient animals (Liver Int 2009 ; 29 : 979-987.). The gene expression could be depending on host conditions and human races. So, we compared gene expressions using micro DNA array analysis among Japanese patients and Finn patients (Genes Chromosomes Cancer 2010; 49: 28-39.). We proved all above research results and presented the data in both national and international congresses. We also discuss each other to promote further collaboration in future.
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Research Products
(24 results)
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[Journal Article] In vitro transformation of adult rat hepatic progenitor cells into pancreatic endocrine hormone.producing cells.2008
Author(s)
Kawasaki H, Mizuguchi T, Kikkawa Y, Oshima H, Sasaki Y, Tokino T, Kokai Y, Miyazaki J, Katsuramaki T, Mitaka M, Hirata K.
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Journal Title
J Hepatobiliary Pancreat Surg 15
Pages: 310-317
Peer Reviewed
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