2010 Fiscal Year Final Research Report
Functional analysis and clinical application for ApoC-1 in patients with pancreatic cancer
| Project/Area Number |
20591620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Chiba University |
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Principal Investigator |
SUDA Kosuke Chiba University, 医学部附属病院, 助教 (50400908)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHITOMI Hideyuki 千葉大学, 医学部附属病院, 助教 (60375631)
TAKANO Shigetsugu 千葉大学, 医学部附属病院, 助教 (20436380)
KIMURA Fumio 千葉大学, 大学院・医学研究院, 准教授 (70334208)
MIYAZAKI Masaru 千葉大学, 大学院・医学研究院, 教授 (70166156)
SUZUKI Daisuke 千葉大学, 医学部附属病院, 医員 (90422229)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 膵臓外科学 |
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| Research Abstract |
Pancreatic cancer still remains one of the most lethal diseases with increasing incidence. We recently identified Apolipoprotein C-1 (ApoC-1) as the serum biomarker for pancreatic cancer by serum protein profiling using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). The purpose of this study is to confirm whether serum ApoC-1 levels of patients with pancreatic cancer were a valuable marker using convenient ELISA method, and also whether ApoC-1 expression in resected pancreatic cancer tissue correlates with prognosis of patients after surgery.
We analyzed ApoC-1 serum level of 32 patients by two proteomic approaches. We also investigate whether ApoC-1 positive expression in resected tissues of patients with pancreatic cancer showed meaningful results in clinical outcome.
ApoC-1 serum levels were significantly decreased in compared preoperative and postoperative serum of patients both SELDI analysis and ELISA method. ApoC-1 levels of pancreatic cancer patients are significantly higher than that of patients with benign pancreatic disease by ELISA method. Furthermore, the immunohistochemical staining of ApoC-1 indicated that overexpression of ApoC-1 in resected pancreatic cancer tissues showed poor prognosis of patients (log rank-test ; P<0.038).
These results suggested that ApoC-1 positive expression was possible to predict poor prognosis of pancreatic cancer patients after surgery.
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[Journal Article] Increased circulating cell signalling phosphoproteins in sera are useful for the detection of pancreatic cancer.2010
Author(s)
Takano S, Sogawa K, Yoshitomi H, Shida T, Mogushi K, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Kato A, Ishihara T, Tanaka H, Yokosuka O, Nomura F, Miyazaki M.
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Journal Title
Br J Cancer 103(2)
Pages: 223-231
Peer Reviewed
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[Journal Article] One hundred seven consecutive surgical resections for hilar cholangiocarcinoma of Bismuth types II, III, IV between 2001 and 20082010
Author(s)
Miyazaki M, Kimura F, Shimizu H, Yoshidome H, Otuka M, Kato A, Yoshitomi H, Furukawa K, Takeuchi D, Takayashiki T, Suda K, Takano S.
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Journal Title
J Hepatobiliary Pancreat Sci 17(4)
Pages: 470-475
Peer Reviewed
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[Journal Article] Annexin II overexpression predicts rapid recurrence after surgery in pancreatic cancer patients undergoing gemcitabine-adjuvant chemotherapy.2008
Author(s)
Takano S, Togawa A, Yoshitomi H, Shida T, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Kato A, Tomonaga T, Nomura F, Miyazaki M.
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Journal Title
Ann Surg Oncol. 15(11)
Pages: 3157-3168
Peer Reviewed
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