2010 Fiscal Year Final Research Report

Investigation of differentiation of extrapancreatic stem cells to pancreatic β-cells

Research Project

Project/Area Number	20591639
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	Nippon Medical School
Principal Investigator	KUDO Mitsuhiro Nippon Medical School, 医学部, 講師 (20256978)
Co-Investigator(Kenkyū-buntansha)	ISHIWATA Toshiyuki 日本医科大学, 医学部, 准教授 (90203041) NAITO Zenya 日本医科大学, 大学院・医学研究科, 教授 (20237184)
Project Period (FY)	2008 – 2010
Keywords	膵β細胞 / 3次元培養 / インスリン分泌 / 細胞接着
Research Abstract	The differentiation of stem cells to pancreatic ss-cells, which produce and secrete insulin, is confirmed by several experimental studies, and the transplantation of such differentiated cells into patients with diabetes is actively studied. Such stem cell therapy will therefore require a large number of differentiated cells to obtain sufficient curative effects. We demonstrated that spheroid formation and suspension culture of ss-cells enhanced the insulin secretory capacity and sensitivity of ss-cells to sulfonylurea drugs such as tolbutamide via functional changes in insulin-secretion-related molecules.

(2 results)