Development of the specific antibody therapy targeting cancer antigen recognized by tumor infiltrating B lymphocytes.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591680
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: MIZUKAMI Makiko University of Occupational and Environmental Health, Japan, 医学部, 非常勤助教 (10441859)
• Co-Investigator(Kenkyū-buntansha): HANAGIRI Takeshi 産業医科大学, 医学部, 准教授 (30299614) ; SHIGEMATSU Yoshiki 産業医科大学, 医学部, 助教 (10546469) ; KAWAHARA Hiroharu 北九州工業高等専門学校, 細胞工学センター, 教授 (20321515) ; YASUMOTO Kosei 産業医科大学, 医学部, 名誉教授 (30150452)
• Project Period (FY): 2008 – 2010
• Keywords: 抗体療法 / 肺癌 / 腫瘍抗原 / 腫瘍浸潤B細胞 / モノクローナル抗体

Research Abstract

The purpose of this study is to establish the specific antibody therapy targeting cancer antigen "UOEH-LC-1". The recombinant protein of UOEH-LC-1 was manufactured and purified. The B lymphocytes was obtained from mice spleen cells by immunization of UOEH-LC-1 protein. The hybridoma, which produced IgG antibody against UOEH-LC-1, was established by fusion of the B lymphocyte and myeloma cell. As a experimental animal model, the lung cancer cell line A110L was transplanted to SCID mice. Then, the purified monoclonal antibody was repeatedly injected from tail vein. However, the anti-tumor effect was not observed as compared to control mice.

Research Products

• [Journal Article] Overexpression of MACC1 mRNA in lung adenocarcinoma is associated with postoperative recurrence. (2011)
  • Author(s): Shimokawa H, Uramoto H, Onitsuka T, Chundong G, Hanagiri T, Oyama T, Yasumoto K
  • Journal Title: J Thorac Cardiovasc Surg. (in press)
  • Peer Reviewed
• [Journal Article] A randomized phase II trial of adjuvant chemotherapy with bi-weekly carboplatin plus paclitaxel versus carboplatin plus gemcitabine in patients with completely resected non-small cell lung cancer. (2010)
  • Author(s): Uramoto H, Nakanishi R, Nagashima A, Uchiyama A, Inoue M, Osaki T, Yoshimatsu T, Sakata H, Nakanishi K, Yasumoto K
  • Journal Title: Anticancer Res. 30 Pages: 4695-4700
  • Peer Reviewed
• [Journal Article] Immunohistochemical Staining with DeltaNp63 Is Useful for Distinguishing the Squamous Cell Component of Adenosquamous Cell Carcinoma of the Lung. (2010)
  • Author(s): Uramoto H, Hanagiri T
  • Journal Title: Anticancer Res. 30 Pages: 4717-4720
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics of resected adenosquamous cell carcinoma of the lung : risk of coexistent double cancer. (2010)
  • Author(s): Uramoto H, Yamada S, Hanagiri T
  • Journal Title: J Cardiothorac Surg. 29 Pages: 92
  • Peer Reviewed
• [Journal Article] TS, DHFR and GARFT Expression in Non-squamous Cell Carcinoma of NSCLC and Malignant Pleural Mesothelioma Patients Treated with Pemetrexed. (2010)
  • Author(s): Uramoto H, Onitsuka T, Shimokawa H, Hanagiri T
  • Journal Title: Anticancer Res 30 Pages: 4309-4316
  • Peer Reviewed
• [Journal Article] A Positive Relationship Between Filamin and VEGF in Patients with Lung Cancer. (2010)
  • Author(s): Uramoto H, Akyurek LM, Hanagiri T
  • Journal Title: Anticancer Res 30 Pages: 3939-3944
  • Peer Reviewed
• [Journal Article] Identification of a lung cancer antigen evading CTL attack due to loss of human leukocyte antigen (HLA) class I expression. (2010)
  • Author(s): Baba T, Hanagiri T, Takenoyama M, Shiota H, Shigematsu Y, Ichiki Y, Uramoto H, So T, Yasumoto K
  • Journal Title: Cancer Sci 101 Pages: 2115-2120
  • Peer Reviewed
• [Journal Article] Expression of Dysadherin and Cytokeratin as Prognostic Indicators of Disease-free Survival in Patients with Stage I NSCLC.. (2010)
  • Author(s): Ono K, Uramoto H, Hanagiri T
  • Journal Title: Anticancer Res 30 Pages: 3273-3278
  • Peer Reviewed
• [Journal Article] Correlation between HLA alleles and EGFR mutation in Japanese patients with adenocarcinoma of the lung. (2010)
  • Author(s): Uramoto H, So T, Nagata Y, Kuroda K, Shigematsu Y, Baba T, So T, Takenoyama M, Hanagiri T, Yasumoto K
  • Journal Title: J Thorac Oncol 5 Pages: 1136-1142
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal transition in EGFR-TKI acquired resistant lung adenocarcinoma. (2010)
  • Author(s): Uramoto H, Iwata T, Onitsuka T, Shimokawa H, Hanagiri T, Oyama T
  • Journal Title: Anticancer Res 30 Pages: 2513-2517
  • Peer Reviewed
• [Journal Article] Phase II trial of adjuvant chemotherapy with bi-weekly carboplatin plus paclitaxel in patients with completely resected non-small cell lung cancer. (2010)
  • Author(s): Sugaya M, Uramoto H, Uchiyama A, Nagashima A, Nakanishi R, Sakata H, Nakanishi K, Hanagiri T, Yasumoto K
  • Journal Title: Anticancer Res 30 Pages: 2631-2639
  • Peer Reviewed
• [Journal Article] Time trends of surgical outcome in patients with non-small cell lung cancer. (2010)
  • Author(s): Hanagiri T,, Baba T, So T, Yasuda M, Ono K, So T, Uramoto H, Takenoyama M, Yasumoto K
  • Journal Title: J Thorac Oncol 5 Pages: 825-829
  • Peer Reviewed
• [Journal Article] Results of surgical resection for patients with large cell carcinoma of the lung. (2010)
  • Author(s): Hanagiri T, Oka S, Takenaka M, Yasuda M, Ono K, So T, Uramoto H, Takenoyama M, Yasumoto K
  • Journal Title: Int J Surg 8 Pages: 391-394
  • Peer Reviewed
• [Journal Article] Comprehensive Molecular analyses of lung adenocarcinoma with regard to the epidermal growth factor receptor, K-ras, MET, and hepatocyte growth factor status. (2010)
  • Author(s): Onitsuka T, Uramoto H, Ono K, Takenoyama M, Hanagiri T, Oyama T, Izumi H, Kohno K, Yasumoto K
  • Journal Title: J Thorac Oncol 5 Pages: 591-596
  • Peer Reviewed
• [Journal Article] Acquired resistance to gefitinib : the contribution of mechanisms other than the T790M, MET, and HGF status. (2010)
  • Author(s): Onitsuka T, Uramoto H, Nose N, Takenoyama M, Hanagiri T, Sugio K, Yasumoto K
  • Journal Title: Lung Cancer 68 Pages: 198-203
  • Peer Reviewed
• [Journal Article] Surgical resection for patients with mucinous bronchioloalveolar carcinoma. (2010)
  • Author(s): Oka S, Hanagiri T, Uramoto H, Baba T, Takenaka M, Yasuda M, Ono K, So T, Takenoyama M, Yasumoto K
  • Journal Title: Asian J Surg 33 Pages: 89-93
  • Peer Reviewed
• [Journal Article] Clinical significance of cancer/testis antigens expression in patients with non-small cell lung cancer. (2010)
  • Author(s): Shigematsu Y, Hanagiri T, Shiota H, Kuroda K, Baba T, Mizukami M, So T, Ichiki Y, Yasuda M, So T, Takenoyama M, Yasumoto K
  • Journal Title: Lung Cancer 68 Pages: 105-110
  • Peer Reviewed
• [Journal Article] Identification of ribosomal protein L19 as a novel tumor antigen recognized by autologous cytotoxic T lymphocytes in lung adenocarcinoma. (2010)
  • Author(s): Kuroda K, Takenoyama M, Baba T, Shigematsu Y, Shiota H, Ichiki Y, Yasuda M, Uramoto H, Hanagiri T, Yasumoto K
  • Journal Title: Cancer Sci 101 Pages: 46-53
  • Peer Reviewed
• [Journal Article] 癌免疫療法-最近の話題から- (2009)
  • Author(s): 花桐武志,竹之山光広,安元公正
  • Journal Title: Biotherapy 23 Pages: 401-407
• [Journal Article] Lung cancer-associated tumor antigens and the present status of immunotherapy against non-small-cell lung cancer. (2009)
  • Author(s): Yasumoto K, Hanagiri T, Takenoyama M
  • Journal Title: Gen Thorac Cardiovasc Surg 57 Pages: 449-457
  • Peer Reviewed
• [Journal Article] Malignant mesothelioma-associated antigens recognized by tumor-infiltrating B cells and the clinical significance of the antibody titers. (2009)
  • Author(s): Shigematsu Y, Hanagiri T, Kuroda K, Baba T, Mizukami M, Ichiki Y, Yasuda M, Takenoyama M, Sugio K, Yasumoto K
  • Journal Title: Cancer Sci 100 Pages: 1326-1334
  • Peer Reviewed
• [Journal Article] Clinical significance of HLA class I alleles on postoperative prognosis of lung cancer patients in Japan. (2009)
  • Author(s): Nagata Y, Hanagiri T, Mizukami M, Kuroda K, Shigematsu Y, Baba T, Ichiki Y, Yasuda M, So T, Takenoyama M, Sugio K, Nagashima A, Yasumoto K
  • Journal Title: Lung Cancer 65 Pages: 91-97
  • Peer Reviewed
• [Journal Article] 肺癌の免疫療法の現況と展望 (2009)
  • Author(s): 花桐武志,竹之山光広,安元公正
  • Journal Title: 呼吸器科 15 Pages: 132-138
• [Journal Article] 選択的ワクチン療法:非特異的免疫療法からの脱出?:現状と展望MAGE抗原を用いた免疫療法の可能性 (2009)
  • Author(s): 花桐武志,杉尾賢二,安元公正
  • Journal Title: Oncology 6 Pages: 72-80
• [Journal Article] Association between estrogen receptor-beta expression and epidermal growth factor receptor mutation in the postoperative prognosis of adenocarcinoma of the lung. (2009)
  • Author(s): Nose N, Sugio K, Oyama T, Nozoe T, Uramoto H, Iwata T, Onitsuka T, Yasumoto K
  • Journal Title: J Clin Oncol 27 Pages: 411-417
  • Peer Reviewed
• [Journal Article] Current status and perspectives on immunotherapy for lung cancer.. (2009)
  • Author(s): Hanagiri T, Takenoyama M, Yasumoto K
  • Journal Title: Curr.Res.in Cancer 3 Pages: 75-87
• [Journal Article] Significance of Smoking as a Postoperative Prognostic Factor in Patients with Non-small Cell Lung Cancer.. (2008)
  • Author(s): Hanagiri T, Sugio K, Mizukami M, Ichiki Y, Sugaya M, Yasuda M, Takenoyama M, Yasumoto K
  • Journal Title: J Thorac Oncol 3 Pages: 1127-1132
  • Peer Reviewed
• [Presentation] Diagnostic markers for malignatn pleural mesothelinoma : Serum antibody against antigens recognized by antibodies produced from tumor infiltraring B cells. (2010)
  • Author(s): Shigematsu Y, Kuroda K, Baba T, Ichiki Y, Ono K, Yasuda M, Uramoto H, So T, Takenoyama M, Hanagiri T, Yasumoto K
  • Organizer: The 10th International Conferenceoftne International Mesothelinoma Interest Group
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100831-20100903
• [Presentation] T細胞受容体移入γδT細胞による養子免疫療法への応用 (2010)
  • Author(s): 重松義紀,黒田耕志,馬場哲郎,永田好香,下川秀彦,小野憲司,浦本秀隆,竹之山光広,花桐武志,安元公正
  • Organizer: 第14回日本がん免疫学会総会
  • Place of Presentation: 熊本
  • Year and Date: 20100722-20100723
• [Presentation] T細胞受容体移入γδT細胞による治療効果の検討 (2010)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第31回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 20100520-20100521
• [Presentation] 局所進行期乳癌に対する術後補助療法としてのHER2蛋白ワクチンの検討 (2010)
  • Author(s): 黒田耕志,竹之山光広,馬場哲郎,重松義紀,宗哲哉,市来嘉伸,小野憲司,安田学,菅谷将一,浦本秀隆,宗知子,花桐武志,安元公正
  • Organizer: 第31回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 20100520-20100521
• [Presentation] 悪性胸膜中皮腫関連抗原の同定と機能解析 (2010)
  • Author(s): 重松義紀,黒田耕志,馬場哲郎,宗哲哉,安田学,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20100513-20100514
• [Presentation] 肺癌に高発現する新規腫瘍抗原ribosomal protein L19の機能解析 (2010)
  • Author(s): 黒田耕志,竹之山光広,塩田広宣,馬場哲郎,重松義紀,宗哲哉,水上真紀子,市来嘉伸,小野憲司,安田学,菅谷将一,浦本秀隆,宗知子,花桐武志,安元公正
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20100513-20100514
• [Presentation] 癌精巣抗原KK-LC-1を認識するT細胞受容体移入γδT細胞を用いた養子免疫療法への応用 (2010)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20100408-20100410
• [Presentation] 悪性胸膜中皮腫に対する腫瘍特異的免疫応答の解析 (2010)
  • Author(s): 竹之山光広
  • Organizer: 第28回産業医科大学学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2010-10-12
• [Presentation] Immunotherapy for Lung Cancer. (2009)
  • Author(s): 安元公正
  • Organizer: 11th Meeting of Chinese Lung Cancer
  • Place of Presentation: Tianjin, China
  • Year and Date: 20090827-20090829
• [Presentation] The identification of tumor antigens associated with asbestos-related lung cancer. (2009)
  • Author(s): Hanagiri T, Yasuda M, Kuroda K, Shigematsu Y, Baba T, So T, Ono K, Uramoto H, So T, Takenoyama M, Yasumoto K
  • Organizer: 13th World Conferenceon Lung Cancer
  • Place of Presentation: San Francisco, USA.
  • Year and Date: 20090731-20090804
• [Presentation] 石綿関連肺癌に対する液性免疫応答の解析 (2009)
  • Author(s): 安田学,花桐武志,黒田耕志,鬼塚貴光,重松義紀,馬場哲郎,永田好香,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,安元公正
  • Organizer: 第13回日本がん免疫学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20090624-20090625
• [Presentation] 肺癌における免疫標的の探索と治療への応用 (2009)
  • Author(s): 安元公正
  • Organizer: 第13回日本がん免疫学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20090624-20090625
• [Presentation] 肺癌に対する新規抗体療法の開発 (2009)
  • Author(s): 安田学,花桐武志,水上真紀子,塩田広宣,黒田耕志,重松義紀,馬場哲郎,永田好香,宗哲哉,市来嘉伸,菅谷将一,浦本秀隆,宗知子,大賀丈史,竹之山光広,杉尾賢二,安元公正
  • Organizer: 第26回日本呼吸器外科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20090514-20090515
• [Presentation] 石綿関連肺癌に対する液性免疫応答の解析 (2008)
  • Author(s): 安田学,重松義紀,塩田広宣,黒田耕志,馬場哲郎,永田好香,水上真紀子,市来嘉伸,大賀丈史,大神明,森本泰夫,濱田哲夫,竹之山光広,花桐武志,杉尾賢二,安元公正
  • Organizer: 第49回日本肺癌学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20081114-20081115
• [Presentation] 悪性胸膜中皮腫におけるSEREX法による腫瘍抗原の同定と新規腫瘍マーカーとしての可能性 (2008)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,永田好香,水上真紀子,市来嘉伸,安田学,竹之山光広,花桐武志,杉尾賢二,安元公正
  • Organizer: 第61回日本胸部外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20081012-20081015
• [Presentation] 石綿関連肺癌に対する新規バイオマーカーの探求 (2008)
  • Author(s): 安田学,重松義紀,塩田広宣,黒田耕志,馬場哲郎,永田好香,水上真紀子,市来嘉伸,野添忠浩,竹之山光広,花桐武志,杉尾賢二,安元公正
  • Organizer: 第25回日本呼吸器外科学会総会
  • Place of Presentation: 宇都宮
  • Year and Date: 20080529-20080530
• [Presentation] 石綿関連肺癌に対する液性免疫応答の解析 (2008)
  • Author(s): 安田学,重松義紀,花桐武志,塩田広宣,黒田耕志,馬場哲郎,永田好香,水上真紀子,市来嘉伸,野添忠浩,竹之山光広,大神明,森本泰夫,杉尾賢二,安元公正
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
  • Year and Date: 20080515-20080517
• [Presentation] 非小細胞肺癌における癌/精巣抗原発現の解析および臨床因子との相関 (2008)
  • Author(s): 花桐武志,重松義紀,塩田広宣,黒田耕志,馬場哲郎,水上真紀子,市来嘉伸,安田学,野添忠浩,竹之山光広,杉尾賢二,安元公正
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
  • Year and Date: 20080515-20080517
• [Presentation] 悪性胸膜中皮腫に関連する新規腫瘍抗原の同定と血清診断への応用 (2008)
  • Author(s): 花桐武志,重松義紀,塩田広宣,黒田耕志,馬場哲郎,永田好香,水上真紀子,市来嘉伸,安田学,大賀丈史,竹之山光広,杉尾賢二,安元公正
  • Organizer: 第26回産業医科大学学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2008-10-30
• [Presentation] 石綿関連肺癌に対する腫瘍抗原の探索と診断及び治療への応用(第2報) (2008)
  • Author(s): 安田学,花桐武志,重松義紀,塩田広宣,黒田耕志,馬場哲郎,大賀丈史,竹之山光広,濱田哲夫,大神明,森本泰夫,杉尾賢二,安元公正
  • Organizer: 第26回産業医科大学学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2008-10-30