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2010 Fiscal Year Final Research Report

Translational Research for Clinical Application of Autologous Bone Marrow Stromal Cell Transplantation for CNS disorders

Research Project

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Project/Area Number 20591701
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionHokkaido University

Principal Investigator

HIDA Kazutoshi  Hokkaido University, 大学院・医学研究科, 准教授 (10238305)

Co-Investigator(Kenkyū-buntansha) KURODA Satoshi  北海道大学, 病院, 講師 (10301904)
SHICHINOHE Hideo  北海道大学, 病院, 助教 (80374479)
IWASAKI Yoshinobu  北海道大学, 大学院・医学研究科, 名誉教授 (00113522)
Project Period (FY) 2008 – 2010
Keywords神経再生 / 骨髄間質細胞 / 脊髄損傷 / 脳損傷 / 脳梗塞 / 再生医療
Research Abstract

For these 3 years, we have run many experiments and explored many new knowledge on autologous bone marrow stromal cell transplantation for central nervous system disorders. We have reported the novel aspects of their biological features and the utility of bioimaging technology such as MRI and optical imaging. This report shows the brief summary of these knowledge.

  • Research Products

    (52 results)

All 2011 2010 2009 2008

All Journal Article (25 results) (of which Peer Reviewed: 23 results) Presentation (22 results) Book (5 results)

  • [Journal Article] Therapeutic impact of human bone marrow stromal cells (hBMSC) expanded by animal serum-free medium for cerebral infarct in rats.2011

    • Author(s)
      Sugiyama T, Kuroda S, Takeda Y, Nishio M, Ito M, Shichinohe H, Koike T
    • Journal Title

      Neurosurgery Epub ahead of print

    • Peer Reviewed
  • [Journal Article] 脳梗塞再生医療の展望2011

    • Author(s)
      黒田敏、宝金清博
    • Journal Title

      Medical Practice 28

      Pages: 681-684

    • Peer Reviewed
  • [Journal Article] Near infrared fluorescence labeling allows noninvasive tracking of bone marrow stromal cells transplanted into rat infarct brain.2011

    • Author(s)
      Sugiyama T, Kuroda S, Osanai T, Shichinohe H, Kuge Y, Ito M, Kawabori M, Iwasaki Y
    • Journal Title

      Neurosurgery Epub ahead of print

    • Peer Reviewed
  • [Journal Article] Pretreatment with the cyclosporine derivative, NIM811, reduces delayed neuronal death in the hippocampus after transient forebrain ischemia.2010

    • Author(s)
      Hokari M, Kuroda S, Iwasaki Y
    • Journal Title

      J Pharm Pharmacol 62

      Pages: 485-490

    • Peer Reviewed
  • [Journal Article] Non-invasive transplantation of bone marrow stromal cell for ischemic stroke: Preliminary study using a thermoreversible gelation polymer (TGP) hydrogel.2010

    • Author(s)
      Osanai T, Kuroda S, Yasuda H, Chiba Y, Maruichi K, Hokari M, Sugiyama T, Shichinohe H, Iwasaki Y
    • Journal Title

      Neurosurgery 66

      Pages: 1140-1147

    • Peer Reviewed
  • [Journal Article] Overexpression of mitochondrial transcription factor A (TFAM) ameliorates delayed neuronal death due to transient forebrain ischemia in mice.2010

    • Author(s)
      Hokari M, Kuroda S, Kinugawa S, Ide T, Tsutsui H, Iwasaki Y
    • Journal Title

      Neuropathology 30

      Pages: 401-407

    • Peer Reviewed
  • [Journal Article] Synergistic effects of bone marrow stromal cells and a Rho kinase (ROCK) inhibitor, Fasudil on axon regeneration in rat spinal cord injury.2010

    • Author(s)
      Chiba Y, Kuroda S, Shichinohe H, Hokari M, Osanai T, Maruichi K, Yano S, Hida K, Iwasaki Y
    • Journal Title

      Neuropathology 30

      Pages: 241-250

    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cell transplantation attenuates cognitive dysfunction due to chronic cerebral ischemia in rats.2010

    • Author(s)
      Shichinohe H, Kuroda S, Sugiyama T, Ito M, Kawabori M
    • Journal Title

      Dement Geriatr Cogn Disord 30

      Pages: 293-301

    • Peer Reviewed
  • [Journal Article] 脳梗塞をターゲットとした再生医療における最適な細胞移植法2010

    • Author(s)
      川堀真人、黒田敏、七戸秀夫、宝金清博
    • Journal Title

      再生医療 9

      Pages: 477-486

    • Peer Reviewed
  • [Journal Article] 脳卒中に対する骨髄間質細胞移植研究の現状と課題2010

    • Author(s)
      黒田敏、七戸秀夫、杉山拓、伊東雅基、川堀真人、千葉泰弘、長内俊也、丸一勝彦、宝金清博、岩崎喜信
    • Journal Title

      脳卒中 32

      Pages: 532-537

    • Peer Reviewed
  • [Journal Article] 中枢神経再生におけるバイオイメージング2010

    • Author(s)
      杉山拓、伊東雅基、七戸秀夫、黒田敏
    • Journal Title

      再生医療 9

      Pages: 65-71

    • Peer Reviewed
  • [Journal Article] Biodegradable fibrin scaffold enhances survival, migration and differentiation of transplanted bone marrow stromal cells in cortical freezing injury model of rats.2010

    • Author(s)
      Yasuda H, Kuroda S, Shichinohe H, Kamei S, Kawamura R, Iwasaki Y
    • Journal Title

      J Neurosurg 112

      Pages: 336-344

    • Peer Reviewed
  • [Journal Article] Transplanted bone marrow stromal cells improves cognitive dysfunction due to diffuse axonal injury in rats.2009

    • Author(s)
      Maruichi K, Kuroda S, Chiba Y, Hokari M, Shichinohe H, Hida K, Iwasaki Y
    • Journal Title

      Neuropathology 29

      Pages: 422-432

    • Peer Reviewed
  • [Journal Article] Graded model of diffuse axonal injury for studying head injury-induced cognitive dysfunction in rats.2009

    • Author(s)
      Maruichi K, Kuroda S, Chiba Y, Hokari M, Shichinohe H, Hida K, Iwasaki Y
    • Journal Title

      Neuropathology 29

      Pages: 132-139

    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cells and bone marrow-derived mononuclear cells -Which are suitable as cell source of transplantation for mice infarct brain?2009

    • Author(s)
      Shichinohe H, Kuroda S, Maruichi K, Osanai T, Sugiyama T, Chiba Y, Yamaguchi A, Iwasaki Y
    • Journal Title

      Neuropathology Epub ahead of print

    • Peer Reviewed
  • [Journal Article] Transplanted bone marrow stromal cells promote axonal regeneration and improve motor function in rat spinal cord injury model.2009

    • Author(s)
      Chiba Y, Kuroda S, Maruichi K, Osanai T, Hokari M, Yano S, Shichinohe H, Hida K, Iwasaki Y
    • Journal Title

      Neurosurgery 64

      Pages: 991-1000

    • Peer Reviewed
  • [Journal Article] Synergistic effects of granulocyte-colony stimulating factor on bone marrow stromal cell transplantation for mice cerebral infarct.2009

    • Author(s)
      穂刈正昭, 黒田敏, 千葉泰弘, 丸一勝彦, 岩崎喜信
    • Journal Title

      Cytokine 46

      Pages: 260-266

    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cell transplantation for cerebral infarction - recent advances and perspective.2009

    • Author(s)
      七戸秀夫, 黒田敏
    • Journal Title

      Res Adv in Stroke 1

      Pages: 23-35

    • Peer Reviewed
  • [Journal Article] Fibrin matrix provides a suitable scaffold for bone marrow stromal cells transplanted into injured spinal cord - A novel material for CNS tissue engineering.2009

    • Author(s)
      Itosaka H, Kuroda S, Shichinohe H, Yasuda H, Yano S, Kamei S, Kawamura R, Hida K, Iwasaki Y
    • Journal Title

      Neuropathology 29

      Pages: 248-257

    • Peer Reviewed
  • [Journal Article] Edaravone (MCI-186) scavenges reactive oxygen species and ameliorates tissue damage in mice spinal cord injury model.2008

    • Author(s)
      Aoyama T, Hida K, Kuroda S, Seki T, Yano S, Shichinohe H, Iwasaki Y
    • Journal Title

      Neurol Med Chir (Tokyo) 48

      Pages: 539-545

    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cells differentiate into neurons and promote axon elongation in spinal cord slice cultures: Their role in cell transplantation therapy for spinal cord injury.2008

    • Author(s)
      Shichinohe H, Kuroda S, Tsuji S, Yamaguchi S, Yano S, Lee JB, Kobayashi H, Hokari M, Hida K, Kikuchi S, Iwasaki Y
    • Journal Title

      Neurorehabil Neural Repair 22

      Pages: 447-457

    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cells protect and repair damaged neurons through multiple mechanisms.2008

    • Author(s)
      Hokari M, Kuroda S, Shichinohe H, Yano S, Hida K, Iwasaki Y
    • Journal Title

      J Neurosci Res 86

      Pages: 1024-1035

    • Peer Reviewed
  • [Journal Article] 骨髄間質細胞を用いた損傷脊髄の再生2008

    • Author(s)
      千葉泰弘、黒田敏、穂刈正昭、矢野俊介、飛騨一利、岩崎喜信
    • Journal Title

      リハビリテーション医学 45

      Pages: 356-360

  • [Journal Article] 中枢神経再生におけるバイオマテリアルの役割2008

    • Author(s)
      長内俊也、黒田敏、岩崎喜信
    • Journal Title

      再生医療 7

      Pages: 77-82

    • Peer Reviewed
  • [Journal Article] ラボから急性期脳梗塞の治療へーラジカルスカベンジャーと骨髄細胞移植2008

    • Author(s)
      黒田敏
    • Journal Title

      脳卒中 30

      Pages: 875-879

  • [Presentation] 慢性脳循環不全+骨髄間質細胞移植モデルにおける記憶学習障害の改善2010

    • Author(s)
      七戸秀夫, ら
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島
    • Year and Date
      20100318-20100319
  • [Presentation] ヒト骨髄間質細胞のラット脳梗塞モデルにおける治療効果の検討2010

    • Author(s)
      杉山拓, ら
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島
    • Year and Date
      20100318-20100319
  • [Presentation] ラット脳梗塞モデルへの骨髄間質細胞の最適な移植経路2010

    • Author(s)
      川堀真人, ら
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島
    • Year and Date
      20100318-20100319
  • [Presentation] Bone marrow stromal cell transplantation for chronic cerebral ischemia model2010

    • Author(s)
      七戸秀夫, et al.
    • Organizer
      Internatonal Stroke Conference 2010
    • Place of Presentation
      San Antonio, USA
    • Year and Date
      20100224-20100226
  • [Presentation] Serial Magnetic Resonance Tracking of Human Bone Marrow Stromal Cells Transplanted into Infarct Brain of Rats - Validity of Cell Expansion in Animal Serum-Free Medium2010

    • Author(s)
      Ito M, et al.
    • Organizer
      第60回米国神経外科コングレス
    • Place of Presentation
      San Franciso
    • Year and Date
      2010-10-16
  • [Presentation] Direct or Intravenous Delivery of Bone Marrow Stromal Cells - Which is Better Route for Ischemic Stroke?2010

    • Author(s)
      Kawabori M, et al.
    • Organizer
      第60回米国神経外科コングレス
    • Place of Presentation
      San Franciso
    • Year and Date
      2010-10-16
  • [Presentation] 脳卒中に対する骨髄間質細胞移植研究の現状と課題2010

    • Author(s)
      黒田敏, ら
    • Organizer
      第35回日本脳卒中学会
    • Place of Presentation
      盛岡
    • Year and Date
      2010-04-15
  • [Presentation] Animal serumfree mediumで培養したヒト骨髄間質細胞の脳梗塞治療における有用性 - in vivo study2010

    • Author(s)
      杉山拓、黒田敏, ら
    • Organizer
      第35回日本脳卒中学会
    • Place of Presentation
      盛岡
    • Year and Date
      2010-04-15
  • [Presentation] 骨髄間質細胞におる中枢神経再生の治療戦略2010

    • Author(s)
      黒田敏, ら
    • Organizer
      第10回脳血管疾患治療研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-27
  • [Presentation] 脳梗塞の病態と外科治療2010

    • Author(s)
      黒田敏, ら
    • Organizer
      第3回脳卒中撲滅講演会
    • Place of Presentation
      奈良
    • Year and Date
      2010-01-31
  • [Presentation] Mitochondrial transcription factor Aは虚血による神経細胞障害を軽減する2009

    • Author(s)
      穂刈正昭, ら
    • Organizer
      日本脳循環代謝学会
    • Place of Presentation
      東京
    • Year and Date
      20091106-20091107
  • [Presentation] 自己骨髄細胞による損傷脊髄の再生2009

    • Author(s)
      黒田敏, ら
    • Organizer
      日本脊髄障害医学会総会
    • Place of Presentation
      札幌
    • Year and Date
      20091106-20091107
  • [Presentation] Transplanted bone marrow stromal cells promote axonal regeneration and improve motor function in rat spinal cord injury2009

    • Author(s)
      千葉泰弘, et al.
    • Organizer
      American Association of Neurological Surgeons
    • Place of Presentation
      Chicago
    • Year and Date
      20090426-20090501
  • [Presentation] Transplanted bone marrow stromal cells improve cognitive dysfunction due to diffuse axonal injury in rats2009

    • Author(s)
      丸一勝彦, et al.
    • Organizer
      American Association of Neurological Surgeons
    • Place of Presentation
      Chicago
    • Year and Date
      20090426-20090501
  • [Presentation] 脳卒中に対する骨髄間質細胞移植研究の現状と課題2009

    • Author(s)
      黒田敏, ら
    • Organizer
      日本脳卒中学会
    • Place of Presentation
      松江
    • Year and Date
      20090320-20090322
  • [Presentation] Bone marrow stromal cell transplantation for central nervous system disorders2009

    • Author(s)
      黒田敏, et al.
    • Organizer
      International Symposium for Future Drug Discovery and Medical Care
    • Place of Presentation
      Sapporo
    • Year and Date
      20090313-20090314
  • [Presentation] 長波長蛍光物質で標識した骨髄間質細胞の頭蓋内光イメージング2009

    • Author(s)
      杉山拓, ら
    • Organizer
      日本再生医療学会
    • Year and Date
      20090305-20090306
  • [Presentation] 骨髄間質細胞の移植はびまん性軸策損傷による高次脳機能障害を改善する2009

    • Author(s)
      丸一勝彦, ら
    • Organizer
      日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      20090305-20090306
  • [Presentation] 骨髄間質細胞移植のバイオマテリアルとしてのMebiol gelの有用性について2009

    • Author(s)
      長内俊也, ら
    • Organizer
      日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      20090305-20090306
  • [Presentation] 塩酸ファスジルは脊髄損傷に対する骨髄間質細胞の移植効果を増強するか?2009

    • Author(s)
      千葉泰弘, ら
    • Organizer
      日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      20090305-20090306
  • [Presentation] 骨髄間質細胞におる中枢神経再生の治療戦略2009

    • Author(s)
      黒田敏, ら
    • Organizer
      第9回ブレインアタックカンファレンス研究会
    • Place of Presentation
      福岡
    • Year and Date
      2009-10-30
  • [Presentation] 骨髄間質細胞におる中枢神経再生の治療戦略2009

    • Author(s)
      黒田敏, ら
    • Organizer
      第12回浜松脳神経障害とフリーラジカル研究会
    • Place of Presentation
      浜松
    • Year and Date
      2009-07-08
  • [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development(Tamaki N and Kuge Y (ed))(Transplanted bone marrow stromal cells improves cognitive dysfunction due to diffuse axonal injury in rats.)2010

    • Author(s)
      Maruichi K, Kuroda S, Chiba Y, Osanai T, Sugiyama T, Hokari M, Hida K, Iwasaki Y
    • Total Pages
      158-164
    • Publisher
      Springer
  • [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development(Tamaki N and Kuge Y (ed))(Beneficial effects of bone marrow stromal cell transplantation on axonal regeneration in injured spinal cord.)2010

    • Author(s)
      Chiba Y, Kuroda S, Maruichi K, Osanai T, Hokari M, Yano S, Shichinohe H, Hida K, Iwasaki Y
    • Total Pages
      152-157
    • Publisher
      Springer
  • [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development(Tamaki N and Kuge Y (ed))(Thermoreversible gelation polymer (TGP) hydrogel as a degradable scaffold for bone marrow stromal cell transplantation.)2010

    • Author(s)
      Osanai T, Kuroda S, Yasuda H, Chiba Y, Maruichi K, Hokari M, Sugiyama T, Shichinohe H, Iwasaki Y
    • Total Pages
      145-151
    • Publisher
      Springer
  • [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development(Tamaki N and Kuge Y (ed))(Non-invasive optical tracking of bone marrow stromal cells transplanted into rat cerebral infarct.)2010

    • Author(s)
      Sugiyama T, Kuroda S, Osanai T, Maruichi K, Chiba Y, Shichinohe H, Kuge Y, Tamaki N, Iwasaki Y
    • Total Pages
      139-144
    • Publisher
      Springer
  • [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development(Tamaki N and Kuge Y (ed))(Bone marrow stromal cell transplantation for central nervous system disorders - Perspective for translational research and clinical application.)2010

    • Author(s)
      Kuroda S, Kuge Y, Tamaki N, Iwasaki Y
    • Total Pages
      126-138
    • Publisher
      Springer

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Published: 2012-01-26   Modified: 2016-04-21  

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