2010 Fiscal Year Final Research Report
Chronological morphological changes in central nervous system induced by chronic electrical sensorimotor cortex stimulation in rats
| Project/Area Number |
20591725
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nihon University |
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Principal Investigator |
OSHIMA Hideki Nihon University, 医学部, 講師 (20328735)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Akiko 日本大学, 医学部, 講師 (30246889)
FUKAYA Chikashi 日本大学, 医学部, 准教授 (50287637)
KATAYAMA Yoichi 日本大学, 医学部, 教授 (00125048)
KOSHINAGA Morimichi 日本大学, 医学部, 兼任講師 (30267067)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 細胞・組織 / 脳・神経 / 神経刺激 |
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| Research Abstract |
Motor cortex stimulation (MCS) has been utilized as a treatment option for various disorders such as medically refractory pain, poststroke hemiplegia, and movement disorders. However, the precise mechanisms underlying its effects remain unknown. In this study, the effects of long-term chronic MCS were investigated by observing the changes in astrocytes. A quadripolar stimulation electrode was implanted on the dura over the sensorimotor cortex of adult rats, and the cortex was continuously stimulated for three hours, one week, four weeks, and eight weeks. Immunohistochemical staining of microglia (ionized calcium-binding adaptor molecule 1 (Iba1) immunoreactive staining) and astrocytes (glial fibrillary acidic protein (GFAP) -immunoreactive staining), and neuronal degeneration histochemistry (Fluoro-Jade B (FJB) staining) were carried out to investigate the morphological changes following long-term chronic MCS. Iba1 staining and FJB staining showed no evidence of Iba1-positive microglial changes or neurodegeneration. Following continuous MCS, GFAP-positive astrocytes were enlarged and their number increased in the cortex and the thalamus of the stimulated hemisphere. These findings indicate that chronic electrical stimulation can continuously activate astrocytes and result in morphological and quantitative changes. These changes may contribute to the mechanisms underlying the neuroplasticity effect induced by MCS.
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