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2012 Fiscal Year Final Research Report

Molecular and morphological study of SDF-1 in tumor proliferation of pituitary adenoma

Research Project

  • PDF
Project/Area Number 20591726
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionNippon Medical School

Principal Investigator

YOSHIDA Daizo  日本医科大学, 医学部, 准教授 (30210701)

Co-Investigator(Kenkyū-buntansha) TERAMOTO Akira  日本医科大学, 医学系大学院・, 教授 (50133070)
YAMAGUCHI Fumio  日本医科大学, 医学部, 講師 (70267219)
TAKUMI Ichiro  日本医科大学, 医学部, 講師 (60307923)
KIM Kyonsong  日本医科大学, 医学部, 助教 (30339387)
Project Period (FY) 2008 – 2012
Keywords低酸素 / SDF-1 / CXCR4 / 下垂体腺腫
Research Abstract

In the present study, we aimed to develop a novel transfection method for short interference RNA (siRNA) to employ nanotube by the surfactant activity.A6K consisting of six alanine residues and hydrophilic head, lysine, was compared with conventional cationic transfectant reagent, siFECTOR and Lipofectamine 2000. Cyto-toxity for human rat pituitary adenoma cell line, AtT-20 or GH3 was examined with MTS assay. Transfection efficiency was analyzed with FITC-labelled siRNA targeting SDF-1 receptor mRNA with fluorescent activity and microscopy. Ultra-structure of A6K was observed with electron microscopy.Non-cytotoxic level of A6K (IC50, 320.8 ・g/ml) was significantly higher than that of siFECTOR ((IC50, 35.6 ・g/ml) and Lipofectamine 2000 (IC50, 22.4 ・g/ml). Transfection efficiency for the siRNA was elevated in a dose- and time- dependent fashion. Relative expression of SDF-1 and its receptor, CXCR4 and CXCR7 mRNA to ・-actin was reduced in a dose-dependent manner and hypoxia in a real-time RT-PCR study. Ultra-structure of A6K was transformed to micelle formation when siRNA was mixed.Lipid-like self assembling peptide, A6K includes genes in the micelle due to hydrophilic tail. This transfection is a novel and stable method with lower cytotoxity. In conclusion, SDF-1 and its receptor-mediated cell signal pathway is enhanced in hypoxic condition.

  • Research Products

    (17 results)

All 2013 2012 2011 2010 2009 2008

All Journal Article (11 results) Presentation (5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A transfection method for short interference RNA with thelipid-like self-assembling nanotube A6K.2013

    • Author(s)
      Yoshida D, Kim K, Takumi I, Yamaguchi F, Teramoto A.
    • Journal Title

      Medical Molecular Morphology

      Volume: (in press)

  • [Journal Article] Clinical clerkship course for medical students on lumbar puncture usingsimulators.2012

    • Author(s)
      Adachi K, Yoshimura A, Aso R, Miyashita T, Yoshida D, Teramoto A, Shimura T.
    • Journal Title

      J Nippon Med Sch.

      Volume: 79(6) Pages: 430-437

  • [Journal Article] Early computed tomography signs as early predictors of hemorrhagic transformation under heparinization in patients with cardiogenic embolism.2012

    • Author(s)
      Terao T, Mishina M, Takumi I, Komaba Y, Mizunari T, Kobayashi S, Yoshida D, Teramoto A.
    • Journal Title

      Geriatr Gerontol Int.

      Volume: 12(3) Pages: 418-424

  • [Journal Article] Digital imaging for statistical analysis of tissuemicroarrays.2011

    • Author(s)
      Yoshida D, Teramoto A.
    • Journal Title

      J Nihon Med Sch.

      Volume: 78(6) Pages: 338-339

  • [Journal Article] Transcranial Doppler ultrasonography for diagnosis of cerebral vasospasm after aneurysmal subarachnoid hemorrhage: mean blood flow velocity ratio of the ipsilateral and contralateral middle cerebral arteries.2011

    • Author(s)
      Nakae R, Yokota H, Yoshida D, Teramoto A.
    • Journal Title

      Neurosurgery.

      Volume: 69(4) Pages: 876-883

  • [Journal Article] Selective posterior decompression of the cervical spine.2011

    • Author(s)
      Kim K, Isu T, Sugawara A, Matsumoto R, Isobe M, Morimoto D, Mishina M, Kobayashi S, Yoshida D, Teramoto A.
    • Journal Title

      Neurol Med Chir (Tokyo).

      Volume: 51(2) Pages: 108-112

  • [Journal Article] The CXCR4 antagonist AMD3100 suppresses hypoxia-mediated growth hormone production in GH3 rat pituitary adenoma cells.2010

    • Author(s)
      Yoshida D, Koketshu K, NomuraR, Teramoto A.
    • Journal Title

      J Neurooncol.

      Volume: 100(1) Pages: 51-64

  • [Journal Article] Concomitant cranial and lumbar subdural hematomas -case report-.2010

    • Author(s)
      Kim K, Katsuno M, Isu T, Mishina M, Yoshida D, Kobayashi S, Teramoto A.
    • Journal Title

      Neurol Med Chir (Tokyo).

      Volume: 50(5) Pages: 402-404

  • [Journal Article] Intracerebral hemorrhage caused by a neoplastic aneurysm from pleomorphic lung carcinoma.2009

    • Author(s)
      Nomura R, Yoshida D, Kim K, Kobayashi S, Teramoto A.
    • Journal Title

      Neurol Med Chir (Tokyo).

      Volume: 49(1) Pages: 33-36

  • [Journal Article] Signaling Pathway Mediated by CXCR7, an Alternative Chemokine Receptor for Stromal-Cell DerivedFactor-1alpha, in AtT20 mouse ACTH-secreting pituitary adenoma cells.2009

    • Author(s)
      Yoshida D, Nomura R, Teramoto A.
    • Journal Title

      J Neuroendocrinol.

      Volume: 21(5) Pages: 481-485

  • [Journal Article] Stromal cell-derived factor-1 expression in pituitary adenoma tissues and upregulation in hypoxia.2009

    • Author(s)
      Nomura R, Yoshida D, Teramoto A.
    • Journal Title

      J Neurooncol.

      Volume: 94(2) Pages: 173-181

  • [Presentation] Alternative pathway by miRNA relating growth hormone synthesis in GH3, rat pituitary adenoma cells.2011

    • Author(s)
      Yoshida D, Koketsu K, Teramoto A.
    • Organizer
      2011Annual Meeting of Congress of Neurosurgical Society
    • Place of Presentation
      Washington, DC, USA
    • Year and Date
      20110000
  • [Presentation] The CXCR4 antagonist AMD3100 suppresses hypoxia-mediated growth hormone production in GH3 rat pituitary adenoma cells.2010

    • Author(s)
      Yoshida D, Koketshu K, Nomura R, Teramoto A.
    • Organizer
      NeuroTalk-2010
    • Place of Presentation
      Singapore
    • Year and Date
      20100000
  • [Presentation] Suppression of Growth Hormone Production by Inhibin, antagonist for CXCR4, a Receptor for Stromal Cell-derived Factor -1.2009

    • Author(s)
      Koketshu K, Yoshida D, Teramoto A.
    • Organizer
      Annual Meeting of the Congress of Neurological Surgeons
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      20090000
  • [Presentation] novel transfection method for short interference RNA with lipid-like self-assembling nanotube, A6K.2009

    • Author(s)
      Yoshida D, Koketshu K, Teramoto A.
    • Organizer
      Annual Meeting of The Congress of Neurological Surgeons
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      20090000
  • [Presentation] Signaling pathway by SDF-1/CXCR4 in pituitary adenoma.2008

    • Author(s)
      Yoshida D, Nomura R, Teramoto A.
    • Organizer
      Annual Meeting of American Association of Neurological Surgeons
    • Place of Presentation
      Chicago, USA
    • Year and Date
      20080000
  • [Patent(Industrial Property Rights)] トランスフェクション剤2009

    • Inventor(s)
      吉田大蔵、武井次郎
    • Industrial Property Number
      PCT/JP2009/064814
    • Filing Date
      2009-08-26

URL: 

Published: 2014-08-29   Modified: 2014-12-03  

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