Effects of bFGF-incorporated gelatin hydrogel and bone marrow-derived Schwann cell transplantation after spinal cord contusion injury in rats

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591736
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: OKAWA Akihiko Chiba University, 大学院・医学研究院, 講師 (30312945)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Masashi 千葉大学, 大学院・医学研究院, 准教授 (50281712) ; MURATA Atsushi 千葉大学, 医学部附属病院, 准教授 (20344997)
• Project Period (FY): 2008 – 2010
• Keywords: spinal cord injury / gelatin hydrogel / bFGF / allodynia / BBB score / BDA

Research Abstract

We investigated the effects of a sustained-release system of bFGF from gelatin hydrogel in a rat spinal cord contusion model.
One week after spinal cord contusion, gelatin hydrogel (GH) containing bFGF (20μg) was injected into the lesion epicenter of rats. Locomotor recovery was assessed over nine weeks by BBB testing. Sensory abnormalities in the hind paws of all the rats were evaluated at five, seven, and nine weeks.
Although BBB scores were better in the bFGF-GH and GH groups than in the saline group, the differences did not reach statistical significance. The bFGF-GH group showed significantly less sensory abnormalities than the saline group at seven weeks.
Our study findings suggest that bFGF-incorporated gelatin hydrogel could have therapeutic potential for alleviating mechanical allodynia following spinal cord injury.

Research Products

• [Journal Article] Transplantation of human bone marrow stromal cell-derived Schwann cells reduces cystic cavity and promotes functional recovery after contusion injury of adult rat spinal cord. (2011)
  • Author(s): Kamada T, Okawa A, Yamazaki M.
  • Journal Title: Neuropathology 31 Pages: 48-58
  • Peer Reviewed
• [Journal Article] Transplantation of murine induced pluripotent stem (iPS) cell-derived astrocytes increases sensitivity to mechanical stimulus in a rat spinal cord injury model
  • Author(s): Hayashi K., Hashimoto M., Koda M., Naito A., Murata A., Okawa A., Takahashi K., Yamazaki Y.
  • Journal Title: J Neurosurgery Spine in press
  • Peer Reviewed
• [Presentation] EFFECTS OF BFGF INCORPORATED GELATIN HYDROGEL AND BONE MARROW STROMAL CELLDERIVED NEURAL PROGENITOR CELL TRANSPLANTATION IN A RAT SPINAL CORD CONTUSION MODEL (2009)
  • Author(s): Hashimoto M., Furuya T., Koda M., Murata A., Okawa A., Yamazaki M., Dezawa M., Matsuse D., Tabata Y., Takahashi K.
  • Organizer: Neurotrauma
  • Place of Presentation: Santa Barbara, California
  • Year and Date: 20090907-20090911
• [Presentation] Effects of bFGF incorporated gelatin hydrogel transplantation in a rat spinal cord contusion (2008)
  • Author(s): Furuya T., Hashimoto M., Koda M., Murata A., Okawa A., Yamazaki M., Dezawa M., Matsuse D., Tabata Y., Takahashi K.
  • Place of Presentation: Orlando, FL
  • Year and Date: 20080727-20080730
• [Presentation] ラット脊髄圧挫損傷モデルにおけるbFGF徐放ゼラチンハイドロゲル移植の検討 (2008)
  • Author(s): 古矢丈雄, 橋本将行, 国府田正雄, 村田淳, 大河昭彦, 山崎正志, 出澤真理, 松瀬大, 田畑泰彦, 高橋和久
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23