2010 Fiscal Year Final Research Report
The mechanism of the increase of pulmonary endothelial cell permeability induced by the increased sympathetic tone and development of the new therapy for ARDS.
| Project/Area Number |
20591801
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Nagoya University |
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Principal Investigator |
NISHIWAKI Kimitoshi Nagoya University, 医学系研究科, 教授 (10189326)
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| Project Period (FY) |
2008 – 2010
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| Keywords | NPY / ARDS / 肺血管透過性 / 肺水腫 / 交感神経 |
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| Research Abstract |
We investigated the mechanism of the increase of pulmonary endothelial cell permeability induced by the NPY, using transendothelial FITC-labeled albumin permeability assay.
The incubation culture plates were comprised of 2 chambers, the upper chamber utilized a porous membrane and the lower used a 24-well micro plate. HPAEC (Human Pulmonary Artery Endothelial Cells) were seeded in the intercell for 2W. The permeability of the endothelial cell monolayer was assessed by measuring the concentration of FITC-labeled albumin from the upper to the lower chamber for 60minutes. The permeability at each NPY conditions were expressed as % compared to the control condition (no NPY) at 100%. The permeability at NPY 0, 10^<-7>, 10^<-6>, 10^<-5>, 10^<-4>, 10^<-3>, 10^<-2>, 10^<-1>, 1, 10μg/ml were 100, 101, 102, 169, 173, 163, 172, 220, 229, 226% respectively. These results showed that NPY increased the permeability of the HPAEC monolayer in dose-dependent manner.
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