2010 Fiscal Year Final Research Report
Neuroprotective effect of fructose 1,6-diphosphate : a 31P-NMR study
| Project/Area Number |
20591807
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Oita University |
|---|
Principal Investigator |
KITANO Takaaki Oita University, 医学部, 教授 (20211196)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOKUMARU Osamu 大分大学, 医学部, 准教授 (40360151)
YOKOI Isao 大分大学, 医学部, 教授 (80150366)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 病態生理学 / 核磁気共鳴法 / 脳虚血 / 脳保護 / 電子スピン共鳴法 / フルクトース1,6-ニリン酸 / クレアチンリン酸 / ATP |
|---|
| Research Abstract |
Fructose 1,6-diphophate is an intermediate substrate of glycolysis. It is a known neuroprotectant and acts as energy metabolite and a radical scavenger. The neuroprotective capacity of FDP was studied using 31P-nuclear magnetic resonance spectroscopy (^<31>P-NMR). The state of energy metabolism during ischemia-reperfusion insult was evaluated by 31P-NMR. The recovery of phosphocreatine, energetic buffer of ATP in living cells, was significantly higher when brain slices were superfused with 5 mM FDP. The series of our experiments, including 31P-NMR and electron spin resonance spectroscopy, indicated that FDP functions as a neuroprotectant not only as an energy metabolite of glycolysis but also as a radical scavenger.
|
