Developing new treatment modalities of bladder cancer on the basis of microRNA expression profile

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591861
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kagoshima University
• Principal Investigator: KAWAMOTO Ken Kagoshima University, 医歯学総合研究科, 研究員 (80363620)
• Co-Investigator(Kenkyū-buntansha): ENOKIDA Hideki 鹿児島大学, 医歯学総合研究科, 講師 (80347103)
• Research Collaborator: 千代丸 剛 鹿児島大学, 医歯学総合研究科, 大学院生 ; 鑪野 秀一 鹿児島大学, 医歯学総合研究科, 大学院生 ; 吉野 裕史 鹿児島大学, 医歯学総合研究科, 大学院生
• Project Period (FY): 2008 – 2010
• Keywords: マイクロRNA / 膀胱癌 / 腫瘍マーカー

Research Abstract

We screened 157 microRNAs and identified the microRNA profile in clinical bladder cancer. We focused on the down-regulated microRNAs, such as miR-133a, and miR-145, which may have tumor suppressive function. Transfection of the target genes, such as KRT-7, FSCN1, and GST-P1, revealed that these genes might have oncogenic function. On the other hand, the expression levels of the up-regulated microRNAs, such as miR-96, miR-183, were indeed over-expressed in bladder cancer, suggesting that they might be potential biomarkers for bladder cancer.

Research Products

• [Journal Article] MiR-133a induces apoptosis through direct regulation of GSTP1 in bladder cancer cell lines. (2011)
  • Author(s): Uchida Y, Chiyomaru T, Enokida H, Kawakami K, Tatarano S, Kawahara K, Nishiyama K, Seki N, Nakagawa M.
  • Journal Title: Urol Oncol. (有)
• [Journal Article] miR-218 on the genomic loss region of chromosome 4p15.31 functions as a tumor suppressor in bladder cancer. (2011)
  • Author(s): Tatarano S, Chiyomaru T, Kawakami K, Enokida H, Yoshino H, Hidaka H, Yamasaki T, Kawahara K, Nishiyama K, Seki N, Nakagawa M.
  • Journal Title: Int J Oncol. 39 Pages: 13-21
  • Peer Reviewed
• [Journal Article] Restoration of miR-517a expression induces cell apoptosis in bladder cancer cell lines. (2011)
  • Author(s): Yoshitomi T, Kawakami K, Enokida H, Chiyomaru T, Kagara I, Tatarano S, Yoshino H, Arimura H, Nishiyama K, Seki N, Nakagawa M.
  • Journal Title: Oncol Rep. 25 Pages: 1661-1668
  • Peer Reviewed
• [Journal Article] The tumour-suppressive function of miR-1 and miR-133a targeting TAGLN2 in bladder cancer. (2011)
  • Author(s): Yoshino H, Chiyomaru T, Enokida H, Kawakami K, Tatarano S, Nishiyama K, Nohata N, Seki N, Nakagawa M.
  • Journal Title: Br J Cancer. 104 Pages: 808-818
  • Peer Reviewed
• [Journal Article] MiR-96 and miR-183 detection in urine serve as potential tumor markers of urothelial carcinoma : correlation with stage and grade, and comparison with urinary cytology. (2011)
  • Author(s): Yamada Y, Enokida H, Kojima S, Kawakami K, Chiyomaru T, Tatarano S, Yoshino H, Kawahara K, Nishiyama K, Seki N, Nakagawa M.
  • Journal Title: Cancer Sci. 102 Pages: 522-529
  • Peer Reviewed
• [Journal Article] LY6K is a novel molecular target in bladder cancer on basis of integrate genome-wide profiling. (2011)
  • Author(s): Matsuda R, Enokida H, Chiyomaru T, Kikkawa N, Sugimoto T, Kawakami K, Tatarano S, Yoshino H, Toki K, Uchida Y, Kawahara K, Nishiyama K, Seki N, Nakagawa M.
  • Journal Title: Br J Cancer. 104 Pages: 376-386
  • Peer Reviewed
• [Journal Article] miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer. (2010)
  • Author(s): Chiyomaru T, Enokida H, Tatarano S, Kawahara K, Uchida Y, Nishiyama K, Fujimura L, Kikkawa N, Seki N, Nakagawa M.
  • Journal Title: Br J Cancer. 102 Pages: 883-891
  • Peer Reviewed
• [Journal Article] Identification of novel microRNA targets based on microRNA signatures in bladder cancer. (2009)
  • Author(s): Ichimi T, Enokida H, Okuno Y, Kunimoto R, Chiyomaru T, Kawamoto K, Kawahara K, Toki K, Kawakami K, Nishiyama K, Tsujimoto G, Nakagawa M, Seki N.
  • Journal Title: Int J Cancer. 125 Pages: 345-52
  • Peer Reviewed
• [Presentation] MicroRNAs (miR-96 and miR-183) detection serves as urine markers in patients with urothelial carcinoma. (2011)
  • Author(s): Enokida H, Seki N, Nakagawa M.
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] Functional role of LASP1 in bladder cnacer cell viability and its regulation by microRNAs. (2011)
  • Author(s): Chiyomaru T, Enokida H, Seki N, Nakagawa M.
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] Restoration of miR-517a expression induces cell apoptosis in bladder cancer cell lines. (2011)
  • Author(s): Yoshitomi T, Enokida H, Seki N, Nakagawa M.
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] The tumor suppressive microRNA cluster of miR-1 and miR-133a targets oncogenic TAGLN2 gene in bladder cancer. (2011)
  • Author(s): Yoshino H, Enokida H, Seki N, Nakagawa M.
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] miR-218 on the genomic loss region of chromosome 4P15.31 function as a tumor suppressor in bladder cancer. (2011)
  • Author(s): Tatarano S, Enokida H, Seki N, Nakagawa M.
  • Organizer: American Urological Association (AUA) annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2011-05-15
• [Presentation] MiR-133a functions as a tumor suppressor and directly regulates GSTP1 expression in bladder cancer. (2010)
  • Author(s): Uchida Y, Enokida H, Seki N, Nakagawa M.
  • Organizer: AUA annual meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-05-29
• [Presentation] miR-145 and miR-133a function as tumor suppressors and directly regulate FSCN1 expression in bladder cancer. (2010)
  • Author(s): Chiyomaru T, Enokida H, Seki N, Nakagawa M.
  • Organizer: AUA annual meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-05-29
• [Presentation] Identification of differentially expressed mature microRNAs in bladder cancer. (2008)
  • Author(s): Enokida H, Seki N, Nakagawa M.
  • Organizer: European Association of Urology (EAU) annual meeting
  • Place of Presentation: Milan, Iltaly
  • Year and Date: 2008-03-26
• [Patent(Industrial Property Rights)] microRNA発現プロファイリングに基づく膀胱癌の検出方法 (2010)
  • Inventor(s): 関直彦、榎田英樹、中川昌之
  • Industrial Property Rights Holder: 国立大学法人千葉大学、国立大学法人鹿児島大学
  • Industrial Property Number: 特許,特願2010-159384
  • Filing Date: 2010-07-14
• [Patent(Industrial Property Rights)] microRNA発現プロファイリングに基づく膀胱癌の検出方法 (2007)
  • Inventor(s): 関直彦、榎田英樹、中川昌之
  • Industrial Property Rights Holder: 国立大学法人千葉大学、国立大学法人鹿児島大学
  • Industrial Property Number: 特許,特願2007-276191
  • Filing Date: 2007-10-24