Bladder reconstruction by autologous bone-marrow-derived mesenchymal stem cell

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591877
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Shinshu University
• Principal Investigator: ISHIZUKA Osamu Shinshu University, 医学部, 准教授 (20184541)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Tetsuya 信州大学, 医学部, 研究員 (00467143)
• Project Period (FY): 2008 – 2010
• Keywords: 骨髄幹細胞 / 再生医療 / 膀胱

Research Abstract

From the clinical point of view, urethral sphincter reconstruction is important to prevent urinary incontinence in the field of bladder reconstruction. At first, we could culture and proliferate the bone-marrow-derived mesenchymal stem cells derived from the femur of rabbit. The cultured stem cells were implanted into the injured urethral sphincter, and we could found the reconstruction of the urethral sphincter by histological methods (immunohistochemistry, RT-PCR method) and functional study.

Research Products

• [Journal Article] Implantation of autologous bone marrow-derived cells reconstructs functional urethral sphincters in rabbits (2010)
  • Author(s): Imamura, T., Ishizuka, O., Kinebuchi, Y., Kurizaki, Y., Nakayama, T., Ishikawa, M., Nishizawa, O.
  • Journal Title: Tissue Eng.(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Rat bladders augmented with a novel bovine pericardium-derived biomaterial reconstruct functional tissue structures. (2010)
  • Author(s): Mimura, Y., Imamura, T., Kinebuchi, Y., Aizawa, N., Ishizuka, O., Nishizawa, O.
  • Journal Title: LUTS 2 Pages: 76-82
  • Peer Reviewed
• [Journal Article] Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. (2010)
  • Author(s): Kinebuchi, Y., Aizawa, N., Imamura, L Ishizuka, O., Igawa, Y., Nishizawa, O.
  • Journal Title: Int.J.Urol. 17 Pages: 359-368
  • Peer Reviewed
• [Journal Article] The microenvironment of freeze-injured mouse urinary bladders enables successful tissue engineering. (2009)
  • Author(s): Imamura, T., Yamamoto, T., Ishizuka, O., Gotoh, M., Nishizawa, O.
  • Journal Title: Tissue Eng. 15(11) Pages: 3367-3375
  • Peer Reviewed
• [Journal Article] Implantation of mouse bone marrow cells reconstructs layered smooth muscle structures in injured urinary bladder. (2008)
  • Author(s): Imamura, T., Kinebuchi, Y., Ishizuka, O., Seki, S., Igawa, Y., Nishizawa, O.
  • Journal Title: Cell transplantation 17 Pages: 267-278
  • Peer Reviewed
• [Presentation] Implantation of human amniotic mesenchymal cells promotes morphological and functional recovery of the freeze-injured mouse urinary bladder. (2010)
  • Author(s): O Minagawa, T., Imamura, T., Aizawa, N., Ishizuka, O., Igawa, Y., Nishizawa, O.
  • Organizer: 25^<th> EAU 2010.
  • Place of Presentation: Barcelona.
  • Year and Date: 20100416-20100420
• [Presentation] Mice freeze-injured urinary bladders provide a microenvironment for bone ?marrow-derived cells to regenerate smooth muscle layers. (2009)
  • Author(s): Imamura, T., Yamamoto, T., Ishizuka,O., Gotoh, M., Nishizawa,O.
  • Organizer: The 4^<th> Pan-pacific continence society meeting.
  • Place of Presentation: Fukuoka
  • Year and Date: 2009-09-10
• [Presentation] Possibility of regeneration of urinary bladder and urethral sphincter by own bone marrow cell. (2009)
  • Author(s): Ishizuka, O.
  • Organizer: 2009 Asia-European International Academic Forum for Urethral Reconstruction.
  • Place of Presentation: Shanghai.
  • Year and Date: 2009-02-06