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2010 Fiscal Year Final Research Report

Development of Regeneration Therapy of External Urethral Sphincter based on the Regulation of Cytokine Signaling Pathway

Research Project

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Project/Area Number 20591882
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionOita University

Principal Investigator

MIMATA Hiromitsu  Oita University, 医学部, 教授 (60219714)

Co-Investigator(Kenkyū-buntansha) SATO Fuminori  大分大学, 医学部, 准教授 (30305049)
HIRATA Yuji  大分大学, 医学部, 助教 (30295183)
SUMINO YASUHIRO  大分大学, 医学部, 助教 (30325716)
Project Period (FY) 2008 – 2010
Keywords尿失禁 / 外尿道括約筋 / 骨格筋幹細胞 / サイトカイン / シグナル伝達 / アポトーシス
Research Abstract

Fragility of urethral rhabdosphincter is one of the causes for urinary stress incontinence following prostatectomy and in the elderly. To develop new treatment modality of urinary incontinence by regeneration of urethral rhabdosphincter, human satellite cells of urethral rhabdosphincter were separated and cultured to explore the effects of variety of cytokines on them and the signal transduction.
TNF-αdose-dependently suppressed the growth of satellite cells of human urethral rhabdosphincter and induced apoptosis in part, and anti- TNF-αdrugs inhibited these effects. Myostatin, skeletal muscle-specific growth inhibiting factor, was expressed on mRNA and protein levels in satellite cells of human urethral rhabdosphincter. Myostatin suppressed their growth dose-dependently, and its inhibitor, Follistatin, reversed its inhibitory effect.
Regeneration of human urethral rhabdosphincter may be possible with anti-TNF-αdrugs and Myostatin inhibitors and prove a novel therapeutic modality for urinary incontinence.

  • Research Products

    (20 results)

All 2011 2010 2009 2008 Other

All Journal Article (7 results) Presentation (13 results)

  • [Journal Article] 外尿道括約筋の再生に関する筋衛星細胞の役割2011

    • Author(s)
      住野泰弘、花田麻里、三股浩光
    • Journal Title

      排尿障害プラクティス 18

      Pages: 317-324

  • [Journal Article] Growth inhibition and apoptosis induction by tumor necrosis factor-alpha in human urethral rhabdosphincter satellite cells.2010

    • Author(s)
      花田麻里, 住野泰弘, 平田裕二, 佐藤文憲, 三股浩光
    • Journal Title

      J Urol 183

      Pages: 2445-2450

  • [Journal Article] The effects of hepatocyte growth factor and insulin-like growth factor-1 on the myogenic differentiation of satellite cells in human urethral rhabdosphincter.2010

    • Author(s)
      住野泰弘, 花田麻里, 平田裕二, 佐藤文憲, 三股浩光
    • Journal Title

      Neurourol Urodyn 29

      Pages: 470-475

  • [Journal Article] 前立腺全摘出術における外科解剖-尿失禁対策2010

    • Author(s)
      住野泰弘、平井健一、佐藤文憲、三股浩光
    • Journal Title

      泌尿器外科 24

      Pages: 27-31

  • [Journal Article] ヒト外尿道括約筋衛星細胞の増殖制御機構2008

    • Author(s)
      住野泰弘, 平田裕二, 花田麻里, 佐藤文憲, 三股浩光
    • Journal Title

      西日本泌尿器科 70

      Pages: 569-575

  • [Journal Article] Long-term cryopreservation of pyramidalis muscle specimens as a source of striated muscle stem cells for treatment of post-prostatectomy stress urinary incontinence.

    • Author(s)
      Sumino Y, Hirata Y, Hanada M, Akita Y, Sato F, Mimata H
    • Journal Title

      Prostate in press.

  • [Journal Article] SOCS1による炎症性発癌の制御

    • Author(s)
      花田俊勝, 三股浩光
    • Journal Title

      臨床免疫アレルギー科

  • [Presentation] プロテオミクスによる外尿道括約筋特異蛋白の同定2011

    • Author(s)
      秋田泰之
    • Organizer
      第20回泌尿器科分子・細胞研究会
    • Place of Presentation
      津
    • Year and Date
      20110311-20110312
  • [Presentation] ヒト外尿道括約筋におけるMyostatinの発現と機能解析2010

    • Author(s)
      秋田泰之
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      盛岡市
    • Year and Date
      20100427-20100430
  • [Presentation] ヒト外尿道括約筋および肛門挙筋におけるトランスクリプトーム解析2010

    • Author(s)
      花田麻里
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      盛岡市
    • Year and Date
      20100427-20100430
  • [Presentation] ヒト外尿道括約筋におけるMyostatinの発現と機能解析2010

    • Author(s)
      秋田泰之
    • Organizer
      第7回泌尿器科再建再生研究会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-09
  • [Presentation] IGF-I局所投与はラット外尿道括約筋の肥大を誘導する2010

    • Author(s)
      秋田泰之
    • Organizer
      第7回泌尿器科再建再生研究会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-09
  • [Presentation] 外尿道括約筋の再生療法2009

    • Author(s)
      三股浩光
    • Organizer
      第22回日本老年泌尿器科学会
    • Place of Presentation
      京都
    • Year and Date
      2009-05-09
  • [Presentation] Growth Inhibition and Apoptosis induction by TNF-α in Satellite cells of Human Urethral Rhabdosphincte2008

    • Author(s)
      花田麻里
    • Organizer
      第60回日本泌尿器科学会西日本総会
    • Place of Presentation
      北九州市
    • Year and Date
      20081106-20081108
  • [Presentation] ヒト外尿道括約筋衛星細胞における遊走と浸潤いついての検討2008

    • Author(s)
      平田裕二
    • Organizer
      第15回日本排尿機能学会
    • Place of Presentation
      東京
    • Year and Date
      20080911-20080913
  • [Presentation] ヒト外尿道括約筋衛星細胞においてTNF-αはTNF受容体を介してアポトーシスを誘導する2008

    • Author(s)
      花田麻里
    • Organizer
      第15回日本排尿機能学会
    • Place of Presentation
      東京
    • Year and Date
      20080911-20080913
  • [Presentation] ヒト外尿道括約筋衛星細胞におけるTNF-αのアポトーシス誘導2008

    • Author(s)
      花田麻里
    • Organizer
      第17回日本アポトーシス研究会
    • Place of Presentation
      京都
    • Year and Date
      20080801-20080802
  • [Presentation] The effects of hepatocyte growth factor and insulin-like growth factor-1 on the myogenic differentiation of satellite cells in human urethral rhabdosphincter2008

    • Author(s)
      Yasuhiro Sumino
    • Organizer
      AUA
    • Place of Presentation
      Orland, Florida, USA
    • Year and Date
      20080517-20080522
  • [Presentation] LIF(白血病阻害因子)はヒト外尿道括約筋筋芽細胞の遊走活性を誘導する2008

    • Author(s)
      平田裕二
    • Organizer
      第96回日本泌尿器科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20080425-20080427
  • [Presentation] ヒト外尿道括約筋衛星細胞における遊走と浸潤いついての検討2008

    • Author(s)
      平田裕二
    • Organizer
      第5回泌尿器科再建再生研究会
    • Place of Presentation
      仙台
    • Year and Date
      2008-06-27

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Published: 2012-01-26   Modified: 2016-04-21  

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