2010 Fiscal Year Final Research Report
Establishment of the laboratory procedure to judge the quality of the sperm which assumed seminal vesicle protein a marker
| Project/Area Number |
20591899
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | International University of Health and Welfare |
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Principal Investigator |
IWAMOTO Teruaki International University of Health and Welfare, 大学病院, 教授 (60046117)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kaoru 桐蔭横浜大学, 先端医用工学センター, 講師 (70398973)
YOSHIIKE Miki 聖マリアンナ医科大学, 医学部, 研究技術員 (60398964)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 精子 / 精子運動抑制因子 / 精子無力症 / 検査法 / 生体分子 |
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| Research Abstract |
For a diagnosis of the asthenozoospermia, the only method is conventional measurement of sperm motility rate under a microscope. We investigate the association between Semenogelin(Sg) and spermatozoa in infertile men with asthenozoospermia using a anti-Sg antibody. Spermatozoa, washed by Percoll density gradients, were immunostained with anti- Sg antibody and subjected to flow cytometric analysis. The proportion of spermatozoa labeled with the antibody and the average intensity of fluorescence labeling per spermatozoa were analyzed in relation to the parameters used for semen analysis. A significant negative correlation was found between sperm motility and the proportion and intensity of labeling. The method presented in this study allows reproducible and objective assessment of the asthenozoospermia.
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Research Products
(30 results)
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[Journal Article] Functional implications of membrane modification with semenogelins for inhibition of sperm motility in humans.2009
Author(s)
Yoshida K, Krasznai ZT, Krasznai Z, Yoshiike M, Kawano N, Yoshida M, Morisawa M, Toth Z, Bazsane ZK, Marian T, Iwamoto T
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Journal Title
Cell Motil Cytoskeleton 66(2)
Pages: 99-108
Peer Reviewed
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