2010 Fiscal Year Final Research Report
Xenotransplantation from GalT-KO miniture swine to baboon in tolerance induction protocol
| Project/Area Number |
20591900
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SHIMIZU Akira Nippon Medical School, 医学部, 准教授 (00256942)
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| Co-Investigator(Kenkyū-buntansha) |
MASUDA Yukinari 日本医科大学, 医学部, 講師 (70173755)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 腎臓移植 / 異種移植 / 拒絶反応 |
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| Research Abstract |
Xenotransplantation could provide a solution to the critical shortage of organs for transplantation in humans. In attempt to prevent hyperacute rejection (HAR) and acute humoral xenograft rejection (AHXR), α1,3-galactosyltransferase gene knockout (GalT-KO) pigs have been produced in Massachusetts General Hospital, Boston, USA. In heart xenotransplantation from GalT-KO pig into baboon, heterotopic heart grafts could beat in abdominal cavity by 179 days (median 78 days). In kidney xenotransplantation, GalT-KO kidney grafts rejected by day 34 in chronic immunosuppression protocol, but grafts could survive up to 83 days in tolerance inducing protocol. GalT-KO heart and kidney xenografts did not develop hyperacute rejection. However, all GalT-KO heart and kidney grafts in the chronic immunosuppression group were rejected by AHXR with thrombotic microangiopathy. Acute cellular xenograft rejection (ACXR) and chronic rejection also developed. However, in the kidney grafts in tolerance inducing protocol, AHRX, ACXR, and chronic rejection could not be detected in the grafts. Therefore, we conclude that the tolerance inducing protocol is necessary for success the heart and kidney xenotransplantation from GalT-KO pig into baboons.
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Research Products
(20 results)
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[Journal Article] Inhibition of capillary repair in proliferative glomerulonephritis results in persistent glomerular inflammation with glomerular sclerosis.2010
Author(s)
Masuda Y, Shimizu A, Kataoka M, Arai T, Ishikawa A, Du X, Saito SK, Aki K, Nagasaka S, Mii A, Fujita E, Fukuda Y
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Journal Title
Lab Invest 90
Pages: 1468-1481
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[Journal Article] Beneficial Effects of Perioperative Low-Dose Inhaled Carbon Monoxide on Pulmonary Allograft Survival in MHC-Inbred CLAWN Miniature Swine.2010
Author(s)
Sahara H, Shimizu A, Setoyama K, Oku M, Okumi M, Nishimura H, Oriyanhan W, Tasaki M, Scalea J, Wada H, Bando T, Date H, Yamada K
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Journal Title
Transplantation 90
Pages: 1336-1343
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[Journal Article] Reversal of age-related thymic involution by an LHRH agonist in miniature swine.2010
Author(s)
Hirakata A, Okumi M, Griesemer AD, Shimizu A, Nobori S, Tena A, Moran S, Arn S, Boyd RL, Sachs DH, Yamada K
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Journal Title
Transpl Immunol 24
Pages: 76-81
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[Journal Article] Results of gal-knockout porcine thymokidney xenografts.2009
Author(s)
Griesemer AD, Hirakata A, Shimizu A, Moran S, Tena A, Iwaki H, Ishikawa Y, Schule P, Arn JS, Robson SC, Fishman JA, Sykes M, Sachs DH, Yamada K
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Journal Title
Am J Transplant 9
Pages: 2669-2678
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[Journal Article] Rejection of cardiac xenografts transplanted from a1,3-galactosyltransferase gene-knockout (GalT-KO) pigs to baboons.2008
Author(s)
Hisashi Y, Yamada K, Kuwaki K, Tseng Y-L, Dor FJMF, Houser SL, Robson SC, Schuurman H-J, Cooper DKC, Sachs DH, Colvin RB, Shimizu A
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Journal Title
Am J Tranplant 8
Pages: 2516-2526
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[Journal Article] Thrombotic microangiopathy associated with humoral rejection of cardiac xenografts from alpha1,3-galactosyltransferase gene-knockout pigs in baboons.2008
Author(s)
Shimizu A, Hisashi Y, Kuwaki K, Tseng YL, Dor FJ, Houser SL, Robson SC, Schuurman HJ, Cooper DK, Sachs DH, Yamada K, Colvin RB
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Journal Title
Am J Pathol 172
Pages: 1471-1481
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