2010 Fiscal Year Final Research Report
Analysis of the sperm maturation mechanism by androgen signaling
| Project/Area Number |
20591903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
SUZUKI Kichiya Tohoku University, 研究所, 主任研究員 (30422116)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 精巣上体 / 精子成熟 / アンドロゲンレセプター / コンディショナルノックアウトマウス |
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| Research Abstract |
In order to examine androgen-signaling pathway in the epididymis, we have attempted to establish a conditional knock out mice of the androgen receptor gene in the initial segment of the epididymis. We first generated a transgenic mouse carrying a tamoxifen-regulated Cre recombinase gene driven by the epididymis-specific lipocalin gene promoter. When the mice was crossbred with a ROSA26-EGFP mice to confirm the Cre expression in the epididymis, unexpectedly the EGFP expression was recognized in the cauda epididymis. Although the Cre mice was crossbred with a mice carrying a conditional androgen receptor allele to generate a conditional androgen receptor knock out mice in the epididymis, the mice demonstrated a normal sperm concentration and mobility.
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[Journal Article] The Role of Foxa Proteins in the Regulation of Androgen Receptor Activity.2009
Author(s)
De Graff DJ, Yu X, Sun Q, Mirosevich J, Jin RJ, Wang Y, Gupta A, Nandana S, Case T, Paul M, Huang HY, Shapiro E, Logan S, Suzuki K, Orgebin-Crist MC, Matusik RJ
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Journal Title
Androgen Action in Prostate Cancer(Tindall D.and Mohler J.eds)(Springer Science)
Pages: 587-615
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[Journal Article] Intramanchette transport during primate spermiogenesis : expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis.2008
Author(s)
Hayasaka S, Terada Y, Suzuki K, Murakawa H, Tachibana I, Sankai T, Murakami T, Yaegashi N, Okamura K
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Journal Title
Asian J Androl 10
Pages: 561-8
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