2010 Fiscal Year Final Research Report
ovarian cancer-specific vaccine therapy by carrier cell
| Project/Area Number |
20591952
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Ehime University |
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Principal Investigator |
HAMADA Katsuyuki Ehime University, 医学部附属病院, 講師 (90172973)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 婦人科腫瘍学 |
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| Research Abstract |
GFP-introduced non small cell lung cancer A549 cells infected with oncolytic adenovirus, AdE3-IAI.3B was injected into the mouse subcutaneous tumors and DNA contents of GFP and AdE3-IAI.3B in the tumors peaked one day later and disappeared two weeks later. EHK-2 cell was established from A549 cells by limiting dilution and showed the 10 time higher antitumor effect than the original A549 cells. OVHM subcutaneous tumor formation was rejected in 40% of mice after the subcutaneous injection of AdE3-IAI.3B-infected EHK-2 carrier cells. This indicated that xenogenic antitumor immunity was introduced after the injection of carrier cells. The infection condition of carrier cells at 200MOI (multiplicity of infection) and 33 hours showed the best antitumor effect. The morphology of non-infected carrier cells was not changed but AdE3-IAI.3B-infected carrier cells showed the nuclear membrane lobulation. After the freeze and thaw of carrier cells, the lobulated nuclear membrane was ruptured and the adenovirus particles were released from the nucleus into the cytoplasm. Under these infection conditions, the in vitro antitumor effect increased by 20% and 90% of mouse showed the complete tumor reduction after the prior immunization of adenovirus. In the mouse model of intraperitoneal dissemination of OVHM ovarian cancer cells, the intraperitoneal injection of AdE3-IAI.3B-infected carrier cells did not cure any mice but the carrier cells infected with Ad-mGM-CSF and AdE3-IAI.3B showed the complete tumor reduction in all treated mice after the prior adenoviral immunization. The tumor formation of OVHM ovarian cancer cells was rejected in all complete tumor reduced mice treated with carrier cells and self-antitumor immunity was introduced in these mice.
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[Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells.2011
Author(s)
Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K., Hamakawa, H.
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Journal Title
Oncology Reports 25
Pages: 795-802
Peer Reviewed
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[Journal Article] Improved gene transfer into renal carcinoma cells using adenovirus vector containing RGD motif.2009
Author(s)
Terao, S., Acharya, B., Suzuki, T., Aoi, T., Naoe, M., Hamada, K., Mizuguchi, H. Gotoh, A.
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Journal Title
Anticancer research 29
Pages: 2997-3002
Peer Reviewed
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[Presentation] Intravenous Injection of the carrier-cell based oncolytic adenovirus suppresses the growth of multiple lung tumors in mouse squamous cell carcinoma model.2009
Author(s)
Shirakawa T., Hamada K., Kitajima, S., Mitsuoka, C., Saito A, Matsuoka, T., Adhim, Z., Terao, S., Gotoh, A., Nibu, K., Fujisawa, M., Lee, K.
Organizer
第15回日本遺伝子治療学会
Place of Presentation
大阪大学、大阪
Year and Date
20090609-20090611
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[Presentation] Intravenous injection of allogeneic tumor cell vaccine carrying oncolytic adenovirus (AdE3-IAI.3B) suppresses the growth of mMultiple lung tumors in mouse squamous cell carcinoma model.2009
Author(s)
Shirakawa T., Hamada K., Kitajima K., Mitsuoka C., Morishita N., Yamaoka N., Saito A., Matsuoka T., Adhim Z., Terao S., Gotoh A., Nibu K., Fujisawa M., Lee K., Huang W.
Organizer
12th Annual Meeting of American Society of Gene Therapy.
Place of Presentation
San Diego, USA.
Year and Date
20090527-20090530
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[Presentation] Pre-clinical studies of GMJ2.1 : Carrie cell-based adenoviral oncolytic virotherapy for head and neck squamous cell carcinoma.2008
Author(s)
Shirakawa. T., Hamada K., Kitajima, K., Mitsuoka, C., Jin, H., Morishita, N., Yamaoka, N., Terao S., Gotoh A., Matsuoka, T., Adhim, Z., Nibu, K., Fujisawa, M., Kawabata, M., Wu, J., Zhou, L., Cao, H., Huang, W.
Organizer
International Society for Cell and Gene Therapy of Cancer China Conference.
Place of Presentation
Shijiazhuang, China.
Year and Date
20080919-20080922
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[Remarks] ホームページ等
