2010 Fiscal Year Final Research Report

Clarification of mechanism of division, proliferation, differentiation, and apoptosis of supporting cells in regeneration of cochlear hair cells

Research Project

Project/Area Number	20591973
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Otorhinolaryngology
Research Institution	The University of Tokyo
Principal Investigator	SUZUKI Mitsuya The University of Tokyo, 医学部, 教授 (50302724)
Co-Investigator(Kenkyū-buntansha)	YAMASOBA Tatsuya 東京大学, 医学部, 教授 (60251302) SAKAMOTO Takashi 東京大学, 医学部, 講師 (50323548) KASHIO Akinori 東京大学, 医学部, 助教 (20451809)
Project Period (FY)	2008 – 2010
Keywords	未成熟ラット / 有毛細胞 / 支持細胞 / 増殖 / アポトーシス
Research Abstract	In developing rats, double-staining with antibodies to Caspase 3 and p27 have shown that large numbers of positive staining cells exist in the organ of Corti on 7 days after birth. These findings suggest that apoptosis is promoted and that proliferation was stopped 7 days after birth. The organ of Corti extirpated on 7th day after birth was cultured with 1mM neomycin solution or acidic acid for 5 days. Then, bromodeoxyuridine (BrdU) as a proliferation marker was administered from 3rd day after starting the culture. No BrdU positive staining cell was observed in the supporting cell, suggesting that supporting cells did not proliferate up to 3^<rd> day after missing hair cell.

(4 results)