Development of anti-tumor gene therapy for retinoblastoma using Sendai virus vector and IFNB gene.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592047
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kyushu University
• Principal Investigator: YOSHIKAWA Hiroshi Kyushu University, 大学病院, 助教 (00304808)
• Co-Investigator(Kenkyū-buntansha): YONEMITSU Yoshikazu 九州大学, 大学院・薬学研究院, 客員教授 (40315065)
• Co-Investigator(Renkei-kenkyūsha): SONODA Koh-hei 九州大学, 大学病院, 講師 (10294943)
• Project Period (FY): 2008 – 2010
• Keywords: 眼細胞生物学 / 眼腫瘍学

Research Abstract

We investigated weather anti-tumor gene therapy using M gene deficient Sendai virus vector encoding IFN-β gene (SeV/ΔM-IFN-β) has therapeutic potentials for the treatment of retinoblastoma. Whereas the gene transduction efficiency of SeV/ΔM vector is low in retinoblastoma cell lines, it mediates efficient gene transfer in a retinal pigment epithelium (RPE)-derived cell line. SeV/ΔM-mediated overexpression of IFN-β in RPE suppressed the growth of retinoblastoma cells, suggesting that intraocular IFN-β gene transfer using SeV/ΔM vector may be a useful strategy for preventing tumor growth in retinoblastoma patients.