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2010 Fiscal Year Final Research Report

The treatments of proliferative vitreoretinopathy by inhibition of TGF-b signaling

Research Project

  • PDF
Project/Area Number 20592050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOita University

Principal Investigator

KIMOTO Kenichi  Oita University, 医学部, 講師 (50315339)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Hidekatsu  大分大学, 医学部, 教授 (00222430)
MATSUO Noritaka  大分大学, 医学部, 准教授 (10284788)
Project Period (FY) 2008 – 2010
Keywords眼細胞生物学 / 網膜色素上皮 / 増殖性硝子体網膜症 / コラーゲン / シグナル伝達 / TGF-β
Research Abstract

Transforming growth factor (TGF)-β is a key mediator of proliferative vitreoretinopathy, but the cellular mechanisms by which TGF-β induces extracellular matrix protein (ECM) synthesis are not fully understood. This study examined whether the PI3K/Akt pathway is involved in TGF-β2-induced collagen expression in human retinal pigment epithelial cells. The biochemical blockade of PI3K/Akt activation inhibited TGF-β2-induced type I collagen mRNA expression and type I collagen synthesis. The blockade of PI3K/Akt pathway inhibited the increase in COL1A2 promoter activities when induced by TGF-β2 and reduced TGF-β2 induction of Smad-mut/Luc promoter activity and CAGA12-Luc activity. Moreover, wortmannin increased the TGF-β2-induced Smad7 mRNA expression levels. The PI3K/Akt pathway plays a role in relaying the TGF-β2 signal to induce type I collagen synthesis in the retinal pigment epithelium through Smad-dependent and Smad-independent pathway.

  • Research Products

    (5 results)

All 2010 2008 Other

All Journal Article (1 results) Presentation (2 results) Remarks (2 results)

  • [Journal Article] PI3K/Akt mediates the expression of type I collagen induced by TGF-b2 in human retinal pigment epithelium

    • Author(s)
      Yokoyama K, Kimoto K, Itoh Y, Nakatsuka K, Matsuo N, Yoshioka H, Kubota T.
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol in press.

  • [Presentation] PI3K/Akt mediates the expression of type I collagen induced by TGF-b2 in human retinal pigment epithelium2010

    • Author(s)
      Yokoyama K, Kimoto K, Kubota T
    • Organizer
      World Ophthalmology Congress 2010
    • Place of Presentation
      ベルリン
    • Year and Date
      20100605-20100609
  • [Presentation] 網膜色素上皮細胞のコラーゲン発現におけるPl3K/Akt経路の関与2008

    • Author(s)
      横山勝彦、木許賢一, ほか
    • Organizer
      第112回日本眼科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2008-04-18
  • [Remarks] ホームページ等

  • [Remarks] 大分大学眼科ホームページ

    • URL

      http://www.med.oita-u.ac.jp/ganka/

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Published: 2012-01-26   Modified: 2016-04-21  

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