2010 Fiscal Year Final Research Report
Ophthalmological analysis of vitamin C knockout mice
| Project/Area Number |
20592057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
KUROSAKA Daijiro Iwate Medical University, 医学部, 教授 (20215099)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ビタミンC / ノックアウトマウス / 加齢性眼疾患 / 酸化ストレス / 白内障 |
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| Research Abstract |
(1) In vitamin C knockout mice (SMP30 knockout mice) given the 0.0375 g/L aqueous solution of vitamin C, the lens vitamin C level decreased to approximately 1/3 of that in wild type mice. In vitamin C knockout mice given the 1.5 g/L aqueous solution of vitamin C, the lens vitamin C level was similar to that in wild type mice. Thus, the 2 V vitamin C doses can be viewed as appropriate for achieving V vitamin C deficiency and sufficiency, respectively, in the vitamin C knockout mouse lens.
(2) In SMP30 knockout mice (vitamin C deficiency) group lens opacities induced by UVR-B were significantly more extensive than in SMP30 knockout mice (vitamin C sufficiency) group or in wild type mice group. It was suggested that vitamin C deficiency increases lens susceptibility to UVR-B induced oxidative stress in mice.
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