Analysis of mechanisms of protozoan invasion into the retinal endothelium of congenital toxoplasmic retinochoroiditis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Chiba University
• Principal Investigator: NOROSE Kazumi Chiba University, 大学院・医学研究院, 助教 (30156244)
• Co-Investigator(Kenkyū-buntansha): FURUYA Mitsuko 横浜市立大学, 医学部, 准教授 (10361445)
• Project Period (FY): 2008 – 2010
• Keywords: 眼微生物学 / 感染症学 / トキソプラズマ / ケモカイン / 接着分子

Research Abstract

We established the model of congenital toxoplasmic retinochoroiditis. The expression of IFN-γ, chemokines and adherence molecules in the retina were extremely high after infection. Thus, IFN-γ, chemokines and adherence molecules have important roles on the mechanisms of pathogenesis of congenital toxoplasmic retinochoroiditis. Toxoplasma gondii was detected in the retina and there were cell infiltration around the retinal vessels showing vasculitis and leakage of fluorescence dye in the retina.

Research Products

• [Journal Article] CXCL10 is required to maintain T cell populations and control parasite replication during chronic ocular toxoplasmosis. (2011)
  • Author(s): Norose K, Kikumura A, Luster AD, Hunter CA, Harris TH.
  • Journal Title: Invest Ophthalmol Vis Sci 52 Pages: 389-398
  • Peer Reviewed
• [Journal Article] Enhancement of ultraviolet B-induced skin tumor development in phospholipase Cε-knockout mice is associated with decreased cell death. (2010)
  • Author(s): Oka M, Edamatsu H, Kunisada M, Hu L, Takenaka N, Dien S, Sakaguchi M, Kitazawa R, Norose K, Kataoka T, Nishigori C.
  • Journal Title: Carcinogenesis. 31(10) Pages: 1897-1902
  • Peer Reviewed
• [Journal Article] Protective immunity against lethal anaphylactic reaction in Toxoplasma gondii-infected mice by DNA vaccination with T. gondii-derived heat shock protein 70 gene. (2010)
  • Author(s): Kikumura A, Fang H, Mun HS, Uemura N, Makino M, Sayama Y, Norose K, Aosai F.
  • Journal Title: Parasitol Int 59(2) Pages: 105-111
  • Peer Reviewed
• [Journal Article] A novel method for isolating podocytes using magnetic activated cell sorting. (2010)
  • Author(s): Murakami A, Oshiro H, Kanzaki S, Yamaguchi A, Yamanaka S, Furuya M, Miura S, Kanno H, Nagashima Y, Aoki I, Nagahama K.
  • Journal Title: Nephrol Dial Transplant. 25 Pages: 3884-3890
  • Peer Reviewed
• [Journal Article] Expression of an activated mammalian target of rapamycin (mTOR) in gastroenteropancreatic neuroendocrine tumors. (2010)
  • Author(s): Shida T, Kishimoto T, Furuya M, Nikaido T, Koda K, Takano S, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Tanizawa T, Nakatani Y, Miyazaki M.
  • Journal Title: Cancer Chemother Pharmacol. 65 Pages: 889-893
  • Peer Reviewed
• [Journal Article] 寄生虫の標的臓器別症状からすすめる実地診療-疑い、問診・診断から治療まで一眼科症状 外来を訪れる寄生虫症 (2010)
  • Author(s): 野呂瀬一美
  • Journal Title: Medical Practice 27(9) Pages: 1515-1518
• [Journal Article] 腹腔内再発を繰り返した子宮間葉系腫瘍: Perivascular Epithelioid Cell Tumor (PEComa)の一例 (2010)
  • Author(s): 新野史, 窪田興志, 稲山嘉明, 古屋充子
  • Journal Title: 日本婦人科病理学会誌 1 Pages: 31-33
  • Peer Reviewed
• [Journal Article] Birt-Hogg-Dube症候群 (2010)
  • Author(s): 古賀俊輔, 古屋充子, 中谷行雄
  • Journal Title: 日本臨床 68 Pages: 361-369
  • Peer Reviewed
• [Journal Article] Lung Cysts in Birt-Hogg-Dube Syndrome : Histopathological characteristics and Aberrant Sequence Repeats. (2009)
  • Author(s): Koga S, Furuya M, Takahashi Y, Tanaka R, Yamaguchi A, Yasufuku K, Hiroshima K, Kurihara M, Yoshino I, Aoki I, Nakatani Y.
  • Journal Title: Pathol Int 59 Pages: 720-728
  • Peer Reviewed
• [Journal Article] Metastasis of malignant struma ovarii to the lumbar spine. (2009)
  • Author(s): Yamashita M, Ishii T, Ohtori S, Oikawa Y, Watanabe T, Ito T, Furuya M, Takahashi K.
  • Journal Title: J Clin Neurosci. 17 Pages: 269-272
  • Peer Reviewed
• [Journal Article] Complexity of Tumor Vasculature and Microenvironment. (2009)
  • Author(s): Furuya M, Yonemitsu Y, Aoki I.
  • Journal Title: Curr Pharm Des 15 Pages: 1854-1867
  • Peer Reviewed
• [Journal Article] 胸壁デスモイドの1切除例 (2009)
  • Author(s): 荒井宏雅, 利野靖, 萬哲漢, 湯川寛夫, 和田修幸, 古屋充子, 李進, 中谷行雄, 益田宗孝
  • Journal Title: 日本外科系連合学会誌 34 Pages: 788-794
  • Peer Reviewed
• [Journal Article] Toxoplasma gondii-derived heat shock protein 70 induces lethal anaphylactic reaction through activation of cytosolic phospholipase A and platelet-activating factor via Toll-like receptor 4/myeloid differentiation factor 88. (2008)
  • Author(s): Fang H, Mun HS, Kikumura A, Sayama Y, Norose K, Yano A, Aosai F.
  • Journal Title: Microbiol Immunol 52(7) Pages: 366-374
  • Peer Reviewed
• [Journal Article] In vivo study of toxoplasmic parasitemia using interferon-gamma-deficient mice : Absolute cell number of leukocytes, parasite load and cell susceptibility. (2008)
  • Author(s): Norose K, Naoi K, Fang H, Yano A.
  • Journal Title: Parasitol Int 57(4) Pages: 447-453
  • Peer Reviewed
• [Journal Article] The clinical significance of anti-heat shock cognate protein 71 antibody in myasthenia gravis. (2008)
  • Author(s): Munakata S, Chen M, Aosai F, Kawaguchi N, Nemoto Y, Norose K, Hattori T, Yano A.
  • Journal Title: J Clin Neurosci 15(2) Pages: 158-165
  • Peer Reviewed
• [Journal Article] Pathophysiology of Placentation Abnormalities in Pregnancy-Induced Hypertension. (2008)
  • Author(s): Furuya M, Ishida J, Aoki I, Fukamizu A.
  • Journal Title: Vasc Health Risk Manag. 4 Pages: 1301-1313
  • Peer Reviewed
• [Journal Article] Cancer Neovascularization and Proinflammatory Microenvironments. (2008)
  • Author(s): Furuya M,, Yonemitsu Y.
  • Journal Title: Curr Cancer Drug Target. 8 Pages: 253-265
  • Peer Reviewed
• [Journal Article] Impaired Placental Neovascularization in Mice with Pregnancy-Associated Hypertension. (2008)
  • Author(s): Furuya M,, Ishida J (equal contribution), Inaba S, Kasuya Y, Kimura S, Nemori R, Fukamizu A.
  • Journal Title: Lab Invest 88 Pages: 416-429
  • Peer Reviewed
• [Journal Article] The expression of NeuroD and mASH1 in the gastroenteropancreatic neuroendocrine tumors. (2008)
  • Author(s): Shida T, Furuya M,, Kishimoto T, Nikaido T, Tanizawa T, Koda K, Oda K, Takano S, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Nakatani Y, Miyazaki M.
  • Journal Title: Mod Pathol. 21 Pages: 1363-1370
  • Peer Reviewed
• [Journal Article] Predominance of IgG4+ plasma cells and CD68 positivity in sclerosing angiomatoid nodular transformation (SANT). (2008)
  • Author(s): Nagai Y, Hayama N, Kishimoto T, Furuya M,, Takahashi Y, Otsuka M, Miyazaki M, Nakatani Y.
  • Journal Title: Histopathology. 53 Pages: 495-498
  • Peer Reviewed
• [Journal Article] Transplantation of skin grafts and organs infected with Toxoglasma gondii as a source of toxoplasmosis in immunocompromised mice.
  • Author(s): Belal US, Norose K, Mohamed RM, Naoi K, Yano A.
  • Journal Title: Jpn. J. Inf. Dis. In press
  • Peer Reviewed
• [Presentation] Behavior of T cells in the eye during chronic toxoplasmic retinochoroiditis using two-photonmicroscopy (2011)
  • Author(s): Kazumi Norose, Akitoshi Kikumura, Christopher A.Hunter, Tajie H.Harris.
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 東京東日本大震災のため延期
  • Year and Date: 20110329-20110330
• [Presentation] Distinct expression patterns of CXCR3 variants in tumor and tumor-associated inflammatory diseases of the ovary. (2010)
  • Author(s): 古屋充子, 宮城悦子, 平原史樹, 宮城洋平, 稲山嘉明, 太田一郎, 上大介, 長嶋洋治, 青木一郎
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-20100924
• [Presentation] The role of CXCL10 in ocular toxoplasmosis. (2010)
  • Author(s): Kazumi Norose, Tajie H. Harris, Akitoshi Kikumura, Christopher A. Hunter
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20100822-20100827
• [Presentation] 経過中にCD20陽性を示したangioimmunoblastic T cell lymphomaの一例 (2010)
  • Author(s): 立花崇孝, 富田直人, 古屋充子, 竹内賢吾, 山中正二, 中村直哉, 藤田浩之, 石ケ坪良明
  • Organizer: 第50回日本リンパ網内系学会
  • Place of Presentation: 新潟
  • Year and Date: 20100618-20100619
• [Presentation] The role of CXCL 10 in ocular toxoplasmosis. (2010)
  • Author(s): Kazumi Norose, Tajie H. Harris, Akitoshi Kikumura, Christopher A. Hunter
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川
  • Year and Date: 20100520-20100521
• [Presentation] 横浜市立大学におけるバイオバンク室の運営と課題 (2010)
  • Author(s): 稲山嘉明, 山中正二, 村上あゆみ, 中山崇, 大城久, 海野俊徳, 小野響子, 平沙代子, 矢澤卓也, 下山田博明, 奥寺康司, 長嶋洋治, 古屋充子, 長濱清隆, 北村均, 青木一郎
  • Organizer: 第99回日本病理学会春季総会
  • Place of Presentation: 東京
  • Year and Date: 20100427-20100429
• [Presentation] Magnet activated cell sorting (MACS)を用いた糸球体上皮細胞の単離 (2010)
  • Author(s): 村上あゆみ, 潘勤雅, 大城久, 山中正二, 古屋充子, 長嶋洋治, 青木一郎, 長濱清隆
  • Organizer: 第99回日本病理学会春季総会
  • Place of Presentation: 東京
  • Year and Date: 20100427-20100429
• [Presentation] 卵巣腫瘍におけるINF-γ誘導性ケモカイン受容体CXCR3バリアントの解析 (2010)
  • Author(s): 古屋充子, 米山朋那, 宮城悦子, 田中玲子, 上大介, 長濱清隆, 長嶋洋治, 宮城洋平, 稲山嘉明, 青木一郎
  • Organizer: 第99回日本病理学会春季総会
  • Place of Presentation: 東京
  • Year and Date: 20100427-20100429
• [Presentation] Birt-Hogg-Dube症候群と判明した多発性肺嚢胞の病理学的特徴 (2010)
  • Author(s): 古屋充子, 古賀俊輔, 高橋葉子, 田中玲子, 廣島健三, 岡本龍郎, 吉野一郎, 青木一郎, 中谷行雄
  • Organizer: 第99回日本病理学会春季総会
  • Place of Presentation: 東京
  • Year and Date: 20100427-20100429
• [Presentation] 超音波検査が虫体先進部の検出に有用であったCreeping diseaseの1例 (2010)
  • Author(s): 若林正一郎、中野倫代、外川八英、神戸直智、松江弘之、青才文江、野呂瀬一美
  • Organizer: 日本皮膚科学会第834回東京地方会
  • Place of Presentation: 千葉
  • Year and Date: 2010-12-18
• [Presentation] 妊娠高血圧症候群における胎児・胎盤機能評価マーカーの検討 (2010)
  • Author(s): 古屋充子
  • Organizer: 第391回神奈川県産婦人科医会地方部会
  • Place of Presentation: 平塚
  • Year and Date: 2010-11-13
• [Presentation] 腹腔内再発を繰り返した子宮間葉系腫瘍の一例 (2010)
  • Author(s): 新野史, 窪田興志, 稲山嘉明, 古屋充子
  • Organizer: 第32回日本婦人科病理学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-03
• [Presentation] 子宮頚部絨毛腺管状乳頭腺癌villoglandular papillary adenocarcinomaの一例 (2009)
  • Author(s): 井畑穣, 宮城悦子, 下山田博明, 小林友紀, 杉浦賢, 古屋充子, 北村和久, 山中正二、稲山嘉明, 平原史樹
  • Organizer: 第48回日本臨床細胞学会秋期大会
  • Place of Presentation: 福岡
  • Year and Date: 20091030-20091031
• [Presentation] 卵管癌と子宮頸部腺癌の共存により細胞診断に苦慮した1例 (2009)
  • Author(s): 杉浦賢, 宮城悦子, 助川明子, 古屋充子, 北村和久, 稲山嘉明, 平原史樹
  • Organizer: 第48回日本臨床細胞学会秋期大会
  • Place of Presentation: 福岡
  • Year and Date: 20091030-20091031
• [Presentation] 当院における絨毛性疾患10年の検討 (2009)
  • Author(s): 井畑穰, 鈴木理絵, 助川明子, 杉浦賢, 宮城悦子, 古屋充子, 山中正二, 平原史樹
  • Organizer: 第47回日本癌治療学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20091022-20091024
• [Presentation] 脳生検にて確定診断に至った、Immunocompetent host発症トキソプラズマ脳炎の1例 (2009)
  • Author(s): 大封昌子、三枝隆博、小林勝哉、渡邉究、中谷嘉文、野呂瀬一美、青才文江、宮崎之男
  • Organizer: 第14回日本神経感染症学会総会
  • Place of Presentation: 宇都宮
  • Year and Date: 20091016-20091017
• [Presentation] Expression of CXCR3 variant in ovarian neoplasms. (2009)
  • Author(s): Furuya M, Miyagi E, Hirahara F, Miyagi Y, Inayama Y, Ota I, Kami D, Nagashima Y, Aoki I
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20091001-20091003
• [Presentation] Pathophysiology of the lung cysts caused by BHD mutation in Birt-Hogg-Dube syndrome patients. (2009)
  • Author(s): 古賀俊輔, 古屋充子, 吉野一郎, 廣島健三, 青木一郎, 中谷嘉文
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20091001-20091003
• [Presentation] 妊娠高血圧症候群における胎盤血管新生の病態解析 (2009)
  • Author(s): 古屋充子, 石田純治, 武内利直, 碓井宏和, 生水真紀夫, 中谷行雄, 上大介, 梅澤明弘, 青木一郎, 深水昭吉
  • Organizer: 第30回日本妊娠高血圧学会総会
  • Place of Presentation: 岡山
  • Year and Date: 20090828-20090830
• [Presentation] 妊娠高血圧症モデルマウスにおける胎盤の血管構造変化に関する研究 (2009)
  • Author(s): 石田純治, 古屋充子, 稲葉早紀, 粕谷善俊, 木村定雄, 根守良一, 深水昭吉
  • Organizer: 第30回日本妊娠高血圧学会総会
  • Place of Presentation: 岡山
  • Year and Date: 20090828-20090830
• [Presentation] 子宮体部原発扁平上皮癌の一例 (2009)
  • Author(s): 長谷川哲哉, 宮城悦子, 佐治日青哉, 井畑穣, 鈴木理絵, 助川明子, 平原史樹, 古屋充子, 北村和久, 稲山嘉明
  • Organizer: 第50回日本臨床細胞学会
  • Place of Presentation: 東京
  • Year and Date: 20090626-20090628
• [Presentation] 卵巣腫瘍における血管作動性ケモカイン受容体CXCR3とそのリガンドの発現解析 (2009)
  • Author(s): 米山朋那, 古屋充子, 宮城悦子, 平原史樹, 宮城洋平, 太田一郎, 西川朱實, 長嶋洋治, 青木一郎
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 20090501-20090508
• [Presentation] WT1遺伝子のexon10のナンセンス変異を認め腎移植に至った1例 (2009)
  • Author(s): 村上あゆみ, 長濱清隆, 原田知典, 伊藤秀一, 寺西純一, 江中牧子, 田辺美樹子, 河内香江, 佐々木毅, 野澤昭典, 古屋充子, 長嶋洋治, 青木一郎
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 20090501-20090503
• [Presentation] BHD遺伝子異常に基づく多発性肺嚢胞・嚢胞発症機構に関する分子病理学的考察 (2009)
  • Author(s): 古賀俊輔, 古屋充子, 高橋葉子, 山口淳, 田中玲子, 安福和弘、吉野一郎, 廣島健三, 青木一郎, 中谷行雄
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 20090501-20090503
• [Presentation] 妊娠高血圧症候群における胎盤解析の展望 (2009)
  • Author(s): 古屋充子
  • Organizer: 第389回神奈川県産婦人科医会地方部会
  • Place of Presentation: 相模大野
  • Year and Date: 2009-11-15
• [Presentation] 妊娠高血圧症候群における胎盤血管新生の病態解析 (2009)
  • Author(s): 古屋充子, 石田純治, 武内利直, 碓井宏和, 深水昭吉
  • Organizer: 第388回神奈川県産婦人科医会地方部会
  • Place of Presentation: 横浜
  • Year and Date: 2009-07-04
• [Presentation] Protective immunity against Toxoplasma gondii heat shoek protein 70-indueed anaphylactic reaction by DNA vaccination. (2008)
  • Author(s): Akitoshi Kikumura, Hao Fang, Yusuke Sayama, Masayuki Makino, Noriko Uemura, Kazumi Norose, Akihiko Yano, Fumie Aosai
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 20081201-20081203
• [Presentation] Implication of CXC-chemokines and their receptors for proinflammatory microenvironments of ovarian neoplasms. (2008)
  • Author(s): Furuya M, Suyama T, Usui H, Tanaka N, Shozu M, Miyagi E, Hirahara F, Nagashima Y, Aoki I
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20081028-20081030
• [Presentation] The expression of Neuro D and mash in the gastroenteropancreatic neuroendocrine tumors. (2008)
  • Author(s): Shida T, Furuya M, Kishimoto M, Takano S, Miyazaki M
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20081028-20081030
• [Presentation] 妊娠高血圧症候群における血管新生の病態解析 (2008)
  • Author(s): 古屋充子, 石田純治, 武内利直, 碓井宏和, 中谷行雄, 深水昭吉
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 20080515-20080517
• [Presentation] Birt-Hogg-Dube syndromeが疑われる肺嚢胞性疾患の分子病理学的検討 (2008)
  • Author(s): 古賀俊輔, 古屋充子, 高橋葉子, 田中玲子, 安福和弘, 吉野一郎, 熊坂利夫, 岸本充, 谷澤徹, 廣島健三, 中谷行雄
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 20080515-20080517
• [Presentation] IL-10siRNAによるトキソプラズマ由来HSP70による骨髄由来樹状細胞の成熟とIL-12産生の増強効果 (2008)
  • Author(s): 佐山勇輔、菊村亮暁、房浩、牧野真幸、野呂瀬一美、矢野明彦、青才文江
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎
  • Year and Date: 20080403-20080404
• [Presentation] トキソプラズマ感染マウスのトキソプラズマHSP70誘導アナフィラキシー反応におけるTLR4/MyD88依存性cPLA2/PAF活性化 (2008)
  • Author(s): 房浩、文恵聖、菊村亮暁、佐山勇輔、牧野真幸、野呂瀬一美、矢野明彦、青才文江
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎
  • Year and Date: 20080403-20080404
• [Presentation] トキソプラズマ感染マウスのトキソプラズマHSP70誘導アナフィラキシー死に対するワクチン開発 (2008)
  • Author(s): 菊村亮暁、房浩、佐山勇輔、文恵聖、牧野真幸、野呂瀬一美、矢野明彦、青才文江
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎
  • Year and Date: 20080403-20080404
• [Presentation] A Case of Multiple Pulmonary Cysts Associated with Birt-Hogg-Dube Gene Mutation. (2008)
  • Author(s): Koga S, Furuya M, Takahashi Y, Tanaka R, Yasufuku K, Yamaguchi A, Yoshino I, Kumasaka T, Nakatan Y
  • Organizer: 1st INAUGURAL BHD SYMPOSIUM
  • Place of Presentation: Roskilde
  • Year and Date: 2008-09-03
• [Remarks] ホームページ等
  • URL: http://www.m.chiba-u.ac.jp/class/infection-hostdefense/