Investigation of neural crest stem cell therapy for Hirschsprung's disease

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Keio University
• Principal Investigator: SHIMOJIMA Naoki Keio University, 医学部, 講師 (30317151)
• Co-Investigator(Kenkyū-buntansha): MORIKAWA Yasuhide 慶應義塾大学, 医学部, 教授 (90124958) ; OKANO Hideyuki 慶應義塾大学, 医学部, 教授 (60160694) ; SHIBATA Shinsuke 慶應義塾大学, 医学部, 助教 (70407089) ; NAGOSHI Narihito 慶應義塾大学, 医学部, (助教) (10383837)
• Project Period (FY): 2008 – 2010
• Keywords: 先天性消化器疾患学 / ヒルシュスプルング病

Research Abstract

Hirschsprung's disease and allied diseases are congenital loss or abnormal development of enteric nervous system. We investigated a feasibility of neural crest stem cell transplantation as a novel therapy alternative to surgery. Neural crest stem cells are co-cultured with gut from Hirschsprung's disease model. Survival, migration, and differentiation of transplanted cells were investigated.
To clarify etiologies of these disease, genomic DNA is extracted and mutation analyses were carried out.

Research Products

• [Journal Article] Sox10-Venus mice : a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes. (2010)
  • Author(s): Shibata S, Yasuda A, et al.
  • Journal Title: MolBrain. 3 Pages: 31
  • Peer Reviewed
• [Journal Article] Hirschsprung病 (2010)
  • Author(s): 下島直樹、森川康英
  • Journal Title: 小児科診療 73 Suppl. Pages: 644-646
• [Journal Article] Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad. (2008)
  • Author(s): Nagoshi N, Shibata S, et al.
  • Journal Title: Cell Stem Cell 10 ; 2(4) Pages: 392-403
  • Peer Reviewed
• [Presentation] Isolation, proliferation, and differentiation of neural crest stem/progenitor cells from human gut in normal and motility disorder patients. (2010)
  • Author(s): Naoki Shimojima, Yasuhide Morikawa, Ryuhei Nishikawa, Shinsuke Shibata, Ryo Hotta, Atsuhiro Arisue, Hirofumi Tomita, Fumika Takasato, Masahiro Mori, Yuki Yamamoto, Yasushi Fuchimoto, Hirotoshi Hasegawa, Ken Hoshino, Narihito Nagoshi, Masaya Nakamura, Yumi Matsuzaki, James H.Okano, Hideyuki Okano
  • Organizer: The 4th Scientific Meeting of the Japan-Hungary Surgical Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-11-19
• [Presentation] Isolation, proliferation, and differentiation of neural crest stem/progenitor cells from human gut in normal and motility disorder patients (2010)
  • Author(s): Naoki Shimojima, Yasuhide Morikawa, Ryuhei Nishikawa, Shinsuke Shibata, Ryo Hotta, Atsuhiro Arisue, Hirofumi Tomita, Fumika Takasato, Masahiro Mori, Yuki Yamamoto, Yasushi Fuchimoto, Hirotoshi Hasegawa, Ken Hoshino, Narihito Nagoshi, Masaya Nakamura, Yumi Matsuzaki, James H.Okano, Hideyuki Okano
  • Organizer: 23rd International Symposium on Paediatric Surgical Research.
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-13
• [Presentation] Isolation, proliferation, and differentiation of neural crest stem cells from human gut. (2010)
  • Author(s): Naoki Shimojima, Yasuhide Morikawa, Ryuhei Nishikawa, Shinsuke Shibata, Ryo Hotta, Hirofumi Tomita, Fumika Takasato, Masahiro Mori, Yuki Yamamoto, Yasushi Fuchimoto, Ken Hoshino, Narihito Nagoshi, Masaya Nakamura, Yumi Matsuzaki, James H.Okano, Hideyuki Okano
  • Organizer: Pacific Association of Pediatric Surgeons 43rd Annual meeting
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-05-26
• [Presentation] Investigation of novel cell replacement therapy for Hirschsprung's disease. (2009)
  • Author(s): Shimojima N, Morikawa Y, Nishikawa R, Shibata S, Hotta R, Nagoshi N, Fuchimoto Y, Hasegawa H, Hoshino K, Nakamura M, Matsuzaki Y, Okano HJ, Okano H.
  • Organizer: XI International Small Bowel Transplantation Symposium
  • Place of Presentation: Bologna, Italy
  • Year and Date: 2009-09-10
• [Presentation] Migration, localization, and differentiation of neural crest-derived enteric neural precursor cells in aganglionic gut (2009)
  • Author(s): Shimojima N, Morikawa Y, Nishikawa R, Shibata S, Hotta R, Nagoshi N, Nakamura M, Matsuzaki Y, Okano HJ, Okano H
  • Organizer: 7th Stem cell research symposium
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-05-15
• [Presentation] Investigation of neural crest stem cell therapy for Hirschsprung's disease (2009)
  • Author(s): Shimojima N, Shibata S, Hotta R, Nishikawa R, Nagoshi N, Okano HJ, Morikawa Y, Okano H
  • Organizer: 2nd International Symposium Development of the enteric nervous system : cells, signals, and genes
  • Place of Presentation: London, England
  • Year and Date: 2009-02-24
• [Remarks] ホームページ等
  • URL: http://web.sc.itc.keio.ac.jp/surgery/ps/staff/studies.html