2010 Fiscal Year Final Research Report
Injectable Nano-Scaffolds, Hydroxyapatite-Polymer Nanocomposite Microsphere, Enhance the Therapeutic Angiogenesis by Cell Transplantation
| Project/Area Number |
20592102
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
FUKUMOTO Shinya Osaka City University, 大学院・医学研究科, 講師 (90381996)
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| Co-Investigator(Kenkyū-buntansha) |
KOYAMA Hidenori 兵庫医科大学, 医学部, 准教授 (80301852)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUZONO Tsutomu 近畿大学, 生物理工学部, 教授 (30332406)
OKADA Masahiro 大阪歯科大学, 歯科理工学講座, 助教 (70416220)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 創傷治癒学 / 虚血性難治性潰瘍 / 血管新生療法 |
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| Research Abstract |
Clinical trials demonstrate the effectiveness of cell-based therapeutic angiogenesis against patients with a variety of ischemic disease; however, the clinical success to date has been still limited. We have reported that nano-scaled sintered hydroxyapatite (HAp)-coating on artificial grafts reveals marked cell-adhesiveness, safety and high tissue-affinity. With this nanotechnology, the inorganic, biodegradable and injectable scaffold, HAp-coated poly L-lactic acid (PLLA) microsphere, has been generated : named nano-scaffold (NS). In this study, we examined its usefulness in cell-based therapeutic angiogenesis. Bone marrow-mononuclear cells (BMC) alone, with uncoated PLLAs (LA), or with HAp-coated microspheres (nano-scaffold : NS), were intramuscularly injected into mice ischemic hind-limbs generated by femoral artery occlusion. Kaplan-Meier analysis demonstrated that NS+BMC treatment markedly prevented limb necrosis after the operation (vs. BMC alone, and LA+BMC). Roles of NS to sustain BMC in ischemic tissues were demonstrated by the findings that immunohistochemistry revealed NS and BMC co-localized, and that NS+BMC group exhibited significantly elevated intramuscular levels of proangiogenic cytokines in ischemic tissues as compared with BMC alone. We demonstrated usefulness of injectable scaffold as an enhancer for cell-based therapeutic angiogenesis.
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[Journal Article] Regions of Arterial Stenosis and Clinical Factors Determining Transcutaneous Oxygen Tension in Patients with Peripheral Arterial Disease.2010
Author(s)
Hiroki Ueno, Shinya Fukumoto, Hidenori Koyama, Shinji Tanaka, Takaaki Maeno, Masahiro Murayama, Yoshiko Otsuka, Yohei Mima, Yuji Kawaguchi, Tetsuo Shoji, Masaaki Inaba, Yoshiki Nishizawa
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Journal Title
J Atheroscler Thromb. 17
Pages: 858-869
Peer Reviewed
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[Presentation] Injectable Cell Scaffolds, Hydroxyapatite. Polymer Nanocomposite Microsphere, Enhance the Therapeutic Angiogenesis by Cell Transplantation.2010
Author(s)
Yohei Mima, Shinya Fukumoto, Hidenori Koyama, Masahiro Okada, Shinji Tanaka, Masahiro Murayama, Yohiko Otsuka, Tsutomu Furuzono, Masaaki Inaba, Yoshiki Nishizawa
Organizer
American Diabetes Association's 70th Scientific Sessions
Place of Presentation
Orlando, FL, USA
Year and Date
20100625-20100629
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