2010 Fiscal Year Final Research Report
The mechanism elucidation of pressure ulcer by using visualized microcirculation model
Project/Area Number |
20592106
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plastic surgery
|
Research Institution | Kyorin University |
Principal Investigator |
OHURA Norihiko Kyorin University, 医学部, 講師 (40322424)
|
Co-Investigator(Kenkyū-buntansha) |
SHIBATA Masahiro 芝浦工業大学, システム学部, 教授 (60158954)
|
Project Period (FY) |
2008 – 2010
|
Keywords | 微小循環 / 褥瘡 / 創傷治癒 |
Research Abstract |
Pressure ulcer occurs due to soft tissue irreversible injury by external forces such as perpendicular force, shear stress. The aim of this study demonstrates the effectof three factors which are perpendicular force, shear stress, ischemic reperfusion injury in mice by intravital microscopic techniques. The cremaster muscle wistar rat model can observe the microcirculation in which blood flow is stopped by perpendicular force. Capillary vessel could not crush and occlude by 32mmHg perpendicular force. We newly designed a compact capillaroscopy which was composed with a CCD video-probe equipped acontact-type objective lens and illuminator. In the present study, we evaluated usefulness of the instrument for a bed-side human capillaroscopy to observe the capillary flow in various dermal regions. The influences of tissue compression on the dermal capillary blood flow were also investigated to confirm the utility for clinical applications. Our capillaroscopy visualized the nutritional capillary blood flow in almost all parts of skin surface. Our observations showed that a level of vertical stress similar to arterial pressure was required to stop the capillary flow. From these demonstrations the present CCD video-probe based capillaroscopy would be useful for clinical applications as a bed-side human capillaroscopy.
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