2010 Fiscal Year Final Research Report
The role of vascular endothelial HSP70 on acute lung injury
| Project/Area Number |
20592115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Akita University |
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Principal Investigator |
SAITO Hajime Akita University, 医学部, 講師 (20323149)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMIYA Yosihiro 秋田大学, 大学院・医学系研究科, 准教授 (30239321)
OGAWA Junーichi 秋田大学, 大学院・医学系研究科, 准教授 (20112774)
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| Project Period (FY) |
2008 – 2010
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| Keywords | heat shock protein / 急性肺障害 / 血管内皮細胞 |
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| Research Abstract |
The transendothelial migration of neutrophils is a critical step in acute inflammation. In the present study, to estimate the role of heat shock protein (HSP), a transwell chamber migration assay system incorporating endothelial monolayer was used to examine the numbers of migrating neutrophils. The expression of endothelial vasodilator-stimulated phosphoprotein (VASP), a protein responsible for controlling the geometry of actin-filament, was also estimated at selected times during the neutrophil migration, in vitro. The results showed that pretreating endothelial cells with hydroxyethyl starch (HES) significantly diminished neutrophil migration, quercetin (HSP70 inhibitor) inhibited the effect of HES on neutrophil transendothelial migration, and VASP phosphorylation normally associated with the inhibition of neutrophil transendothelial migration. These data suggest that endothelial VASP regulate transendothelial neutrophil migration by modulating the cytoskeletal events that associated with upstream HSP70 signal transduction mechanisms.
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