Mechanism of salivary secretion and activation of paracellular pathway.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592152
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: HASHIMOTO Sadamitsu Tokyo Dental College, 歯学部, 准教授 (10201708)
• Co-Investigator(Renkei-kenkyūsha): MURAKAMI Masataka 生理学研究所, 細胞器官研究系, 准教授 (10104275) ; SHIMONO Masaki 東京歯科大学, 歯学部, 教授 (00085771)
• Research Collaborator: SUGIYA Hiroshi 日本大学, 生物資源学部, 教授 (20050114)
• Project Period (FY): 2008 – 2010
• Keywords: 唾液腺 / 唾液分泌 / タイト結合 / 細胞骨格 / 傍細胞経路 / isoproterenol / carbachol / 凍結割断法

Research Abstract

To elucidate the morphological change of the paracellular route for fluid secretion, the cytoskeleton of TJs were examined by TEM, using deep etching freeze-fracture (FF) replicas. Isolated submandibular glands (SMGs) were stimulated with 1 μM of carbachol (CCh) and isoproterenol (IPR). After CCh/IPR stimulation, cytoskeletal filaments beneath the plasma membrane were arranged in a thicker layer. Contraction of the submembranous actin cytoskeleton during exocytosis elicited by CCh/IPR may cause rearrangement of TJ strands, and modulate increased paracellular permeability.

Research Products

• [Journal Article] Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium. (2010)
  • Author(s): Sugisawa M., Masaoka T., Enokiya Y., Muramatsu T., Hashimoto S., Yamada S., Shimono M.
  • Journal Title: J Periodont Res. 45(2) Pages: 284-291
  • Peer Reviewed
• [Journal Article] Down-regulated genes in mouse dental papillae and pulp. (2010)
  • Author(s): Sasaki H., Muramatsu T., Kwon H. J., Yamamoto S., Hashimoto S., Jung H. S., Shimono M.
  • Journal Title: J Dent Res. 89(7) Pages: 679-683
  • Peer Reviewed
• [Journal Article] Sodium-calcium exchangers in rat ameloblasts. (2010)
  • Author(s): Okumura R., Shibukawa Y., Muramatsu T., Hashimoto S., Nakagawa K., Tazaki M., Shimono M.
  • Journal Title: J Pharmacol Sci. 112(2) Pages: 223-230
  • Peer Reviewed
• [Journal Article] Proliferation, migration and apoptosis of periodontal ligament cells after tooth replantation. (2010)
  • Author(s): Sato K., Muramatsu T., Tsuchiya Y., Masaoka T., Enokiya Y., Hashimoto S., Shimono M.
  • Journal Title: Oral Dis. 16(3) Pages: 263-268
  • Peer Reviewed
• [Journal Article] A case of congenital midline fistula of the upper lip. (2010)
  • Author(s): Nakano Y., Somiya H., Shibui T., Uchiyama T., Takano N., Shibahara T., Hashimoto S.
  • Journal Title: Bull Tokyo Dent Coll. 51(1) Pages: 31-34
  • Peer Reviewed
• [Journal Article] Morphological evidence of paracellular transport in perfused rat submandibular glands. (2009)
  • Author(s): 橋本貞充, 村上政隆
  • Journal Title: J.Medical Investigation 56 Pages: 395-397
  • Peer Reviewed
• [Journal Article] Morpho-Physiological approach to the paracellular route for salivary secretion by isolated perfused submandibular gland. (2009)
  • Author(s): Murakami M., Hashimoto S., Wei M., Hill A.E.
  • Journal Title: J.Medical Investigation Proceeding 56 Pages: 322-324
  • Peer Reviewed
• [Journal Article] Electrophysiological properties of AQP6 in mouse parotid acinar cells. (2009)
  • Author(s): Ichikawa H., Shibukawa Y., Sahara Y., Tsumura M., Qi B., Satoh K., Narita T., Hashimoto S. Momose Y., Tazaki M., Shimono M., Sugiya H.
  • Journal Title: J.Medical Investigation Proceeding 56 Pages: 347-349
  • Peer Reviewed
• [Journal Article] Role of aquaporins and regulation of secretory vesicle volume in cell secretion. (2008)
  • Author(s): Sugiya H., Matsuki-Fukushima M., Hashimoto S.
  • Journal Title: J Cell Mol Med 12(5A) Pages: 1486-1494
  • Peer Reviewed
• [Journal Article] Presence and localization of aquaporin-6 in rat parotid acinar cells. (2008)
  • Author(s): Matsuki-Fukushima M., Hashimoto S., Shimono M., Satoh K., Fujita-Yoshigaki J, Sugiya H.
  • Journal Title: Cell Tissue Res. 332 Pages: 73-80
  • Peer Reviewed
• [Journal Article] Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland. (2008)
  • Author(s): Kim Y J., Kwon H J., Shinozaki N., Hashimoto S., Shimono M., Cho S W., Jung H.S.
  • Journal Title: Cell Tissue Res. 334(1) Pages: 47-53
  • Peer Reviewed
• [Presentation] Cytoskeletal change of paracellular pathway in perfused rat submandibular glands. (2010)
  • Author(s): Hashimoto S, Murakami M, Matsuki-Fukushima M, Narita T, Shibukawa Y
  • Organizer: 21th Int Sympo on Morphol Scis
  • Place of Presentation: Taormina, Italy
  • Year and Date: 2010-09-21
• [Presentation] 耳下腺分離腺房細胞におけるjasplakinolideのアポトーシス誘導 (2009)
  • Author(s): 福島美和子、橋本貞充、村上政隆、下野正基、杉谷博士
  • Organizer: 日本唾液腺学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 20091200
• [Presentation] マウス耳下腺腺房細胞のAQP6の電気生理学による特性 (2009)
  • Author(s): 市川秀樹、渋川義幸、佐原資謹、祁兵、佐藤啓太郎、成田貴則、橋本貞充、田崎雅和、下野正基、杉谷博士
  • Organizer: 歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 20090900
• [Presentation] Morphological evidence of paracellular transport in perfused rat submandibular glands. (2009)
  • Author(s): 橋本貞充, 村上政隆
  • Organizer: ISES, The 11th International Symposium on Exocrine Secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 20090723-20090725
• [Presentation] Morpho-Physiological approach to the paracellular route for salivary secretion by isolated perfused submandibular gland. (2009)
  • Author(s): Murakami M., Hashimoto S., Wei M., Hill A.E.
  • Organizer: ISES, The 11th International Symposium on Exocrine Secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 20090723-20090725
• [Presentation] Electrophysiological properties of AQP6 in mouse parotid acinar cells. (2009)
  • Author(s): Ichikawa H., Shibukawa Y., Sahara Y., Tsumura M., Qi B., Satoh K., Narita T., Hashimoto S., Momose Y., Tazaki M., Shimono M., Sugiya H.
  • Organizer: ISES, The 11th International Symposium on Exocrine Secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 20090723-20090725
• [Presentation] マウス耳下腺腺房細胞でのaquaporin6 の発現 (2009)
  • Author(s): 市川秀樹、澁川義幸、橋本貞充、田崎雅和、下野正基
  • Organizer: 東京歯科大学学会
  • Place of Presentation: 千葉
  • Year and Date: 20090400
• [Presentation] Hill AE The control of paracellular transport during salivary fluid secretion by the isolated perfused submandibular gland of rat. (2009)
  • Author(s): Murakami M, Hashimoto S, Sugiya H, Narita T, Qi B, Wei M
  • Organizer: 36th Int U Physiol Scis
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-31
• [Presentation] Salivary secretion : assessment of trans- and paracellular transport by physiol-morphological techniques. (2008)
  • Author(s): Murakami M, Hashimoto S, Riva A, Segawa A, Hill AE
  • Organizer: 39th NIPS Int sympo Front Biol Imag
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2008-11-10