2010 Fiscal Year Final Research Report
Elucidation of mechanisms underlying regulation of proliferation and differentiation of mesenchymal stem cells derived from periodontal ligament
| Project/Area Number |
20592169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
ISHISAKI Akira Iwate Medical University, 歯学部, 教授 (20356439)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 幹細胞 / 歯根膜 / 再生医療 |
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| Research Abstract |
To elucidate molecular mechanisms underlying the regulation of proliferation and differentiation of mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL), we established two types of single cell-derived cultures (SCDCs) of rat ligament cells, one of which named as SCDC2 retained the ability of self-renewal and multipotency and another of which named as SCDC1 did not. Then, we comprehensively compared the genes expression status and phosphorylation status of intracellular molecules between SCDC1 and SCDC2. We found that among 54,000 target genes, around 600 genes were identified as differentially expressed genes between the two types of cells. In particular, we found that some transcription factors, which were previously reported as proliferation and differentiation regulators of several kinds of stem cells, were highly expressed in the SCDC2 cells. Moreover, we found that the FGF-induced- and TGF--induced-intracellular signaling played important roles on the regulation of proliferation and differentiation of the MSCs. Thus, we successfully elucidated the molecular mechanisms underlying the regulation of proliferation and differentiation of MSCs derived from PDL.
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[Journal Article] Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function.2011
Author(s)
Kanno Y, Ishisaki A, Kawashita E, Chosa N, Nakajima K, Nishihara T, Toyoshima K, Okada K, Ueshima S, Matsushita K, Matsuo O, Matsuno H.
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Journal Title
J Biol Chem 286
Pages: 8952-8960
Peer Reviewed
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