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2010 Fiscal Year Final Research Report

Imaging analysis of the electrolyte transport and protein secretions in salivary ducts to reveal their regulatory mechanisms

Research Project

  • PDF
Project/Area Number 20592180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

TANIMURA Akihiko  北海道医療大学, 歯学部, 准教授 (70217149)

Co-Investigator(Kenkyū-buntansha) MORITA Takao  北海道医療大学, 歯学部, 講師 (20326549)
NEZU Akihiro  北海道医療大学, 歯学部, 講師 (00305913)
TOJYO Yosuke  北海道医療大学, 歯学部, 教授 (90111731)
Project Period (FY) 2008 – 2010
Keywords歯科薬理学
Research Abstract

Using multiphoton microscopy, the present study established that rat salivary ductal cells exhibit spontaneous oscillations in intracellular Ca^<2+> concentration([Ca^<2+>] i). Pharmacological examinations indicate that purinergic receptors play major roles in these spontaneous Ca2+oscillations. It was also found that spontaneous elevations of[Ca^<2+>] i were well correlated with spontaneous cell swelling of ductal cells. These results indicate the possibility that the Ca^<2+> oscillations are induced by the spontaneous ATP release in salivary ductal cells in the resting state and regulate electrolyte reabsorption.

  • Research Products

    (17 results)

All 2011 2010 2009 2008

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (8 results)

  • [Journal Article] Diversity and spactio-temporal proterties of calcium responses in salivary ducts2011

    • Author(s)
      Tanimura A, Shitara A, and Tojyo Y.
    • Journal Title

      J Oral Biosci

      Volume: 53 Pages: 48-56

    • Peer Reviewed
  • [Journal Article] The development of FRET based IP_3 biosensors and their use for monitoring IP3 dynamics during Ca^<2+> oscillations and Ca2+waves in nonexcitable cells2011

    • Author(s)
      Tanimura A.
    • Journal Title

      J Oral Biosci

      Volume: 53 Pages: 109-121

    • Peer Reviewed
  • [Journal Article] Expression of functional Stiml-mKO1 in rat submandibular acinar cells by retrograde ductal injection of adenoviral vector2011

    • Author(s)
      Morita T, Tanimura A, Shitara A, Suzuki Y, Nezu A, Takuma T, and Tojyo Y.
    • Journal Title

      Arch Oral Biol

      Volume: 56 Pages: 1356-1365

    • Peer Reviewed
  • [Journal Article] Visualization of Ins(1, 4, 5) P_3 dynamics in living cells : two distinct pathways for Ins(1, 4, 5) P_3 generation following mechanical stimulation of HSY-EA1 cells2010

    • Author(s)
      Nezu A, Tanimura A, Morita T, and Tojyo Y.
    • Journal Title

      J Cell Sci

      Volume: 123 Pages: 2292-2298

    • Peer Reviewed
  • [Journal Article] A Stiml-dependent, noncapacitative Ca^<2+>-entry pathway is activated by B-cellreceptor stimulation and depletion of Ca^<2+>2009

    • Author(s)
      Morita T, Tanimura A, Baba B, Kurosaki T, and Tojyo Y.
    • Journal Title

      J Cell Sci

      Volume: 122 Pages: 1220-1228

    • Peer Reviewed
  • [Journal Article] Role of VAMP8/endobrevin in Constitutive Exocytotic Pathway in HeLa Cells2009

    • Author(s)
      Okayama M, Arakawa T, Tanimura A, Mizoguchi I, Tajima Y, and Takuma T.
    • Journal Title

      Cell Struct Funct

      Volume: 34 Pages: 115-125

    • Peer Reviewed
  • [Journal Article] Mechanism of calcium waves and oscillations in non-excitable cells2009

    • Author(s)
      Tanimura A.
    • Journal Title

      International Journal of Oral-Medical Science

      Volume: 8 Pages: 1-11

    • Peer Reviewed
  • [Journal Article] Use of fluorescence resonance energy transfer-based biosensors for the quantitative analysis of inositol 1, 4, 5-trisphosphate dynamics in calcium oscillations2009

    • Author(s)
      Tanimura A, Morita T, Nezu A, Shitara A, Hashimoto N, and Tojyo Y.
    • Journal Title

      J Biol Chem

      Volume: 284 Pages: 8910-8917

    • Peer Reviewed
  • [Journal Article] Spontaneous oscillations in intracellular Ca^<2+> concentration via purinergic receptors elicit transient cell swelling in rat parotid ducts2009

    • Author(s)
      Shitara A, Tanimura A, Sato A, and Tojyo Y.
    • Journal Title

      Am J Physiol

      Volume: 297 Pages: G1198-G1205

    • Peer Reviewed
  • [Presentation] Roles of purinergic receptors on the spontaneous and agonist-induced Ca^<2+> responses in rat salivary ducts2011

    • Author(s)
      Tanimura A.
    • Organizer
      The 8th Gordon Research Conference on Salivary Glands and Exocrine Biology
    • Place of Presentation
      ガルベストン(TX, USA)
    • Year and Date
      20110200
  • [Presentation] 蛍光イメージングによる細胞内カルシウム情報伝達系の研究2010

    • Author(s)
      谷村明彦
    • Organizer
      第52回歯科基礎医学会総会
    • Place of Presentation
      東京都(船堀)
    • Year and Date
      20100900
  • [Presentation] 唾液腺への非侵襲的アデノウイルス注入によるStimlのin vivo発現と機能解析2010

    • Author(s)
      森田貴雄、谷村明彦、設楽彰子、鈴木裕子、根津顕弘、田隈泰信、東城庸介
    • Organizer
      第61回日本薬理学会北部会
    • Place of Presentation
      札幌市
    • Year and Date
      20100900
  • [Presentation] 容量性および非容量性カルシウム流入機構におけるSTIMlの機能2010

    • Author(s)
      谷村明彦、森田貴雄
    • Organizer
      第87回日本生理学会、シンポジウム:ストア作動性カルシウム流入機構とその生理的役割
    • Place of Presentation
      盛岡市
    • Year and Date
      20100500
  • [Presentation] 唾液腺導管細胞のカルシウム応答とその多様性2009

    • Author(s)
      谷村明彦
    • Organizer
      51回歯科基礎医学会学術大会サテライトシンポジウム:-唾液腺研究最前線-口腔乾燥の分子機構と臨床応用
    • Place of Presentation
      新潟
    • Year and Date
      20090900
  • [Presentation] 細胞質発現型IP_3バイオセンサーの精製とそれを用いた耳下腺腺房細胞のIP_3濃度測定への応用2009

    • Author(s)
      根津顕弘、谷村明彦、森田貴雄、東城庸介
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟市
    • Year and Date
      20090900
  • [Presentation] Monitoring of IP_3 dynamics during Ca^<2+> oscillations in HSY human parotid cell line with FRET-based IP_3 biosensors2009

    • Author(s)
      Tanimura A, Morita T, Nezu A, Tojyo Y.
    • Organizer
      The 11^<th> international Symposium on Exocrine Secretion
    • Place of Presentation
      徳島市
    • Year and Date
      20090700
  • [Presentation] ラット耳下腺導管細胞における自発的Ca^<2+>反応の発生機構2008

    • Author(s)
      設楽彰子、谷村明彦、森田貴雄、根津顕弘、東城庸介
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      東京都(品川)
    • Year and Date
      20080900

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Published: 2013-07-31  

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